OCA at 20:34, 12 April 2023

OCA — Wed, 12 Apr 2023 20:34:27 GMT

←Older revision		Revision as of 20:34, 12 April 2023
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-	'''Unreleased structure'''

-	The ~~entry~~ 8gvc is ~~ON HOLD until Paper~~ Publication	+	==The cryo-EM structure of hAE2 with bicarbonate==
		+	<StructureSection load='8gvc' size='340' side='right'caption='[[8gvc]], [[Resolution\|resolution]] 2.89Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8gvc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GVC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gvc OCA], [https://pdbe.org/8gvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gvc RCSB], [https://www.ebi.ac.uk/pdbsum/8gvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gvc ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B3A2_HUMAN B3A2_HUMAN] Sodium-independent anion exchanger which mediates the electroneutral exchange of chloride for bicarbonate ions across the cell membrane (PubMed:15184086, PubMed:34668226). Plays an important role in osteoclast differentiation and function (PubMed:34668226). Regulates bone resorption and calpain-dependent actin cytoskeleton organization in osteoclasts via anion exchange-dependent control of pH (By similarity). Essential for intracellular pH regulation in CD8(+) T-cells upon CD3 stimulation, modulating CD8(+) T-cell responses (By similarity).[UniProtKB:P13808]<ref>PMID:15184086</ref> <ref>PMID:34668226</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cell maintains its intracellular pH in a narrow physiological range and disrupting the pH-homeostasis could cause dysfunctional metabolic states. Anion exchanger 2 (AE2) works at high cellular pH to catalyze the exchange between the intracellular HCO(3)(-) and extracellular Cl(-), thereby maintaining the pH-homeostasis. Here, we determine the cryo-EM structures of human AE2 in five major operating states and one transitional hybrid state. Among those states, the AE2 shows the inward-facing, outward-facing, and intermediate conformations, as well as the substrate-binding pockets at two sides of the cell membrane. Furthermore, critical structural features were identified showing an interlock mechanism for interactions among the cytoplasmic N-terminal domain and the transmembrane domain and the self-inhibitory effect of the C-terminal loop. The structural and cell-based functional assay collectively demonstrate the dynamic process of the anion exchange across membranes and provide the structural basis for the pH-sensitive pH-rebalancing activity of AE2.

-	~~Authors~~:	+	The structural basis of the pH-homeostasis mediated by the Cl(-)/HCO(3)(-) exchanger, AE2.,Zhang Q, Jian L, Yao D, Rao B, Xia Y, Hu K, Li S, Shen Y, Cao M, Qin A, Zhao J, Cao Y Nat Commun. 2023 Mar 31;14(1):1812. doi: 10.1038/s41467-023-37557-y. PMID:37002221<ref>PMID:37002221</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8gvc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Cao Y]]
		+	[[Category: Hu K]]
		+	[[Category: Jian L]]
		+	[[Category: Rao B]]
		+	[[Category: Xia Y]]
		+	[[Category: Yao D]]
		+	[[Category: Zhang Q]]

OCA at 04:09, 26 October 2022

OCA — Wed, 26 Oct 2022 04:09:52 GMT

←Older revision		Revision as of 04:09, 26 October 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8gvc is ON HOLD	+	The entry 8gvc is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8gvc" [edit=sysop:move=sysop]

OCA — Wed, 21 Sep 2022 06:43:58 GMT

Protected "8gvc" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:43, 21 September 2022

OCA: New page: '''Unreleased structure''' The entry 8gvc is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 21 Sep 2022 06:43:57 GMT

New page: '''Unreleased structure''' The entry 8gvc is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8gvc is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8gvc - Revision history

OCA at 20:34, 12 April 2023

OCA at 04:09, 26 October 2022

OCA: Protected "8gvc" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8gvc is ON HOLD Authors: Description: Category: Unreleased Structures