OCA at 08:22, 7 December 2022

OCA — Wed, 07 Dec 2022 08:22:40 GMT

←Older revision		Revision as of 08:22, 7 December 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 8h0v is ~~ON HOLD until Paper~~ Publication	+	==RNA polymerase II transcribing a chromatosome (type I)==
		+	<StructureSection load='8h0v' size='340' side='right'caption='[[8h0v]], [[Resolution\|resolution]] 3.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8h0v]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_phaffii Komagataella phaffii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8H0V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8H0V FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8h0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8h0v OCA], [https://pdbe.org/8h0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8h0v RCSB], [https://www.ebi.ac.uk/pdbsum/8h0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8h0v ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C4R4Y0_KOMPG C4R4Y0_KOMPG] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In chromatin, linker histone H1 binds to nucleosomes, forming chromatosomes, and changes the transcription status. However, the mechanism by which RNA polymerase II (RNAPII) transcribes the DNA in the chromatosome has remained enigmatic. Here we report the cryo-electron microscopy (cryo-EM) structures of transcribing RNAPII-chromatosome complexes (forms I and II), in which RNAPII is paused at the entry linker DNA region of the chromatosome due to H1 binding. In the form I complex, the H1 bound to the nucleosome restricts the linker DNA orientation, and the exit linker DNA is captured by the RNAPII DNA binding cleft. In the form II complex, the RNAPII progresses a few bases ahead by releasing the exit linker DNA from the RNAPII cleft, and directly clashes with the H1 bound to the nucleosome. The transcription elongation factor Spt4/5 masks the RNAPII DNA binding region, and drastically reduces the H1-mediated RNAPII pausing.

-	~~Authors~~:	+	Structural basis of RNA polymerase II transcription on the chromatosome containing linker histone H1.,Hirano R, Ehara H, Kujirai T, Uejima T, Takizawa Y, Sekine SI, Kurumizaka H Nat Commun. 2022 Nov 26;13(1):7287. doi: 10.1038/s41467-022-35003-z. PMID:36435862<ref>PMID:36435862</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8h0v" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Komagataella phaffii]]
		+	[[Category: Large Structures]]
		+	[[Category: Ehara H]]
		+	[[Category: Hirano R]]
		+	[[Category: Kurumizaka H]]
		+	[[Category: Sekine S]]
		+	[[Category: Takizawa Y]]
		+	[[Category: Tomoya K]]

OCA: Protected "8h0v" [edit=sysop:move=sysop]

OCA — Wed, 09 Nov 2022 05:13:28 GMT

Protected "8h0v" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:13, 9 November 2022

OCA: New page: '''Unreleased structure''' The entry 8h0v is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 09 Nov 2022 05:13:27 GMT

New page: '''Unreleased structure''' The entry 8h0v is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8h0v is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

8h0v - Revision history

OCA at 08:22, 7 December 2022

OCA: Protected "8h0v" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8h0v is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures