8hl8 - Revision history

OCA at 19:51, 29 May 2024

OCA — Wed, 29 May 2024 19:51:14 GMT

←Older revision		Revision as of 19:51, 29 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8hl8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinactinospora_thermotolerans Marinactinospora thermotolerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HL8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[8hl8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinactinospora_thermotolerans Marinactinospora thermotolerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HL8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hl8 OCA], [https://pdbe.org/8hl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hl8 RCSB], [https://www.ebi.ac.uk/pdbsum/8hl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hl8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hl8 OCA], [https://pdbe.org/8hl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hl8 RCSB], [https://www.ebi.ac.uk/pdbsum/8hl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hl8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G8HX37_9ACTN G8HX37_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:54, 29 March 2023

OCA — Wed, 29 Mar 2023 06:54:57 GMT

←Older revision		Revision as of 06:54, 29 March 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8hl8 is ~~ON HOLD until 2024~~-11-29	+	==Crystal structrue of MtdL R257K mutant==
		+	<StructureSection load='8hl8' size='340' side='right'caption='[[8hl8]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8hl8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinactinospora_thermotolerans Marinactinospora thermotolerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HL8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hl8 OCA], [https://pdbe.org/8hl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hl8 RCSB], [https://www.ebi.ac.uk/pdbsum/8hl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hl8 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Members of glycosyltransferase family 75 (GT75) not only reversibly catalyze the autoglycosylation of a conserved arginine residue with specific NDP-sugars but also exhibit NDP-pyranose mutase activity that reversibly converts specific NDP-pyranose to NDP-furanose. The latter activity provides valuable NDP-furanosyl donors for glycosyltransferases and requires a divalent cation as a cofactor instead of FAD used by UDP-D-galactopyranose mutase. However, details of the mechanism for NDP-pyranose mutase activity are not clear. Here we report the first crystal structures of GT75 family NDP-pyranose mutases. The novel structures of GT75 member MtdL in complex with Mn(2+) and GDP, GDP-D-glucopyranose, GDP-L-fucopyranose, GDP-L-fucofuranose, respectively, combined with site-directed mutagenesis studies, reveal key residues involved in Mn(2+) coordination, substrate binding, and catalytic reactions. We also provide a possible catalytic mechanism for this unique type of NDP-pyranose mutase. Taken together, our results highlight key elements of an enzyme family important for furanose biosynthesis.

-	~~Authors~~:	+	Structures of the NDP-pyranose mutase belonging to glycosyltransferase family 75 reveal residues important for Mn(2+) coordination and substrate binding.,Du X, Chu X, Liu N, Jia X, Peng H, Xiao Y, Liu L, Yu H, Li F, He C J Biol Chem. 2023 Feb;299(2):102903. doi: 10.1016/j.jbc.2023.102903. Epub 2023 , Jan 13. PMID:36642179<ref>PMID:36642179</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8hl8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Marinactinospora thermotolerans]]
		+	[[Category: He C]]
		+	[[Category: Li FD]]

OCA at 06:08, 11 January 2023

OCA — Wed, 11 Jan 2023 06:08:09 GMT

←Older revision		Revision as of 06:08, 11 January 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8hl8 is ON HOLD	+	The entry 8hl8 is ON HOLD until 2024-11-29

	Authors:		Authors:

OCA: Protected "8hl8" [edit=sysop:move=sysop]

OCA — Wed, 07 Dec 2022 05:59:46 GMT

Protected "8hl8" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:59, 7 December 2022

OCA: New page: '''Unreleased structure''' The entry 8hl8 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Dec 2022 05:59:44 GMT

New page: '''Unreleased structure''' The entry 8hl8 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8hl8 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]