8hnz - Revision history

OCA at 19:51, 29 May 2024

OCA — Wed, 29 May 2024 19:51:42 GMT

←Older revision		Revision as of 19:51, 29 May 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A8I3B027_9ACTN A0A8I3B027_9ACTN]		[https://www.uniprot.org/uniprot/A0A8I3B027_9ACTN A0A8I3B027_9ACTN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Heterodimeric tryptophan-containing diketopiperazines (HTDKPs) are an important class of bioactive secondary metabolites. Biosynthesis offers a practical opportunity to access their bioactive structural diversity, however, it is restricted by the limited substrate scopes of the HTDKPs-forming P450 dimerases. Herein, by genome mining and investigation of the sequence-product relationships, we unveiled three important residues (F387, F388 and E73) in these P450s that are pivotal for selecting different diketopiperazine (DKP) substrates in the upper binding pocket. Engineering these residues in Nas(F5053) significantly expanded its substrate specificity and enabled the collective biosynthesis, including 12 self-dimerized and at least 81 cross-dimerized HTDKPs. Structural and molecular dynamics analysis of F387G and E73S revealed that they control the substrate specificity via reducing steric hindrance and regulating substrate tunnels, respectively.
		+
		+	Engineering the Substrate Specificity of a P450 Dimerase Enables the Collective Biosynthesis of Heterodimeric Tryptophan-Containing Diketopiperazines.,Sun C, Ma BD, Li G, Tian W, Yang L, Peng H, Lin Z, Deng Z, Kong XD, Qu X Angew Chem Int Ed Engl. 2023 Jun 19;62(25):e202304994. doi: , 10.1002/anie.202304994. Epub 2023 May 8. PMID:37083030<ref>PMID:37083030</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 8hnz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 21:27, 28 June 2023

OCA — Wed, 28 Jun 2023 21:27:07 GMT

←Older revision		Revision as of 21:27, 28 June 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8hnz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._NRRL_F-5053 Streptomyces sp. NRRL F-5053]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HNZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[8hnz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._NRRL_F-5053 Streptomyces sp. NRRL F-5053]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HNZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3I5:(3~{S},8~{a}~{S})-3-[(5-fluoranyl-1~{H}-indol-3-yl)methyl]-2,3,6,7,8,8~{a}-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione'>3I5</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3I5:(3~{S},8~{a}~{S})-3-[(5-fluoranyl-1~{H}-indol-3-yl)methyl]-2,3,6,7,8,8~{a}-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione'>3I5</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hnz OCA], [https://pdbe.org/8hnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hnz RCSB], [https://www.ebi.ac.uk/pdbsum/8hnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hnz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hnz OCA], [https://pdbe.org/8hnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hnz RCSB], [https://www.ebi.ac.uk/pdbsum/8hnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hnz ProSAT]</span></td></tr>
	</table>		</table>

OCA at 09:29, 19 April 2023

OCA — Wed, 19 Apr 2023 09:29:41 GMT

←Older revision		Revision as of 09:29, 19 April 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8hnz is ~~ON HOLD until 2024~~-12-09	+	==Crystal structure of cytochrome P450 NasF5053 mutant E73S complexed with 6FCWP==
-		+	<StructureSection load='8hnz' size='340' side='right'caption='[[8hnz]], [[Resolution\|resolution]] 1.50Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[8hnz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._NRRL_F-5053 Streptomyces sp. NRRL F-5053]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HNZ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3I5:(3~{S},8~{a}~{S})-3-[(5-fluoranyl-1~{H}-indol-3-yl)methyl]-2,3,6,7,8,8~{a}-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione'>3I5</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hnz OCA], [https://pdbe.org/8hnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hnz RCSB], [https://www.ebi.ac.uk/pdbsum/8hnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hnz ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A8I3B027_9ACTN A0A8I3B027_9ACTN]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Streptomyces sp. NRRL F-5053]]
		+	[[Category: Kong XD]]
		+	[[Category: Ma BD]]
		+	[[Category: Qu X]]
		+	[[Category: Tian W]]

OCA at 05:04, 25 January 2023

OCA — Wed, 25 Jan 2023 05:04:04 GMT

←Older revision		Revision as of 05:04, 25 January 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8hnz is ON HOLD	+	The entry 8hnz is ON HOLD until 2024-12-09

	Authors:		Authors:

OCA: Protected "8hnz" [edit=sysop:move=sysop]

OCA — Wed, 14 Dec 2022 08:06:56 GMT

Protected "8hnz" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:06, 14 December 2022

OCA: New page: '''Unreleased structure''' The entry 8hnz is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 14 Dec 2022 08:06:55 GMT

New page: '''Unreleased structure''' The entry 8hnz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8hnz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]