OCA at 08:00, 6 December 2023

OCA — Wed, 06 Dec 2023 08:00:59 GMT

←Older revision		Revision as of 08:00, 6 December 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8hrp is ~~ON HOLD until 2025~~-06-15	+	==Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 in complex with NAD and G3P==
		+	<StructureSection load='8hrp' size='340' side='right'caption='[[8hrp]], [[Resolution\|resolution]] 1.99Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8hrp]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum_ATCC_13032 Corynebacterium glutamicum ATCC 13032]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HRP FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.99Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=G3H:GLYCERALDEHYDE-3-PHOSPHATE'>G3H</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hrp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hrp OCA], [https://pdbe.org/8hrp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hrp RCSB], [https://www.ebi.ac.uk/pdbsum/8hrp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hrp ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G3P_CORGL G3P_CORGL] Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.[UniProtKB:P9WN83]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Since the discovery of l-glutamate-producing Corynebacterium glutamicum, it has evolved to be an industrial workhorse. For biobased chemical production, suppling sufficient amounts of the NADPH cofactor is crucial. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme that converts glyceraldehyde-3-phosphate (G3P) to 1,3-bisphosphoglycerate and produces NADH, is a major prospective solution for the cofactor imbalance issue. In this study, we determined the crystal structure of GAPDH from C. glutamicum ATCC13032 (CgGAPDH). Based on the structural information, we generated six CgGAPDH variants, CgGAPDH(L36S), CgGAPDH(L36S/T37K), CgGAPDH(L36S/T37K/P192S), CgGAPDH(L36S/T37K/F100V/P192S), CgGAPDH(L36S/T37K/F100L/P192S), and CgGAPDH(L36S/T37K/F100I/P192S), that can produce both NADH and NAPDH. The final CgGAPDH(L36S/T37K/F100V/P192S) variant showed a 212-fold increase in enzyme activity for NADP as well as 200% and 30% increased activity for the G3P substrate under NAD and NADP cofactor conditions, respectively. In addition, crystal structures of CgGAPDH variants in complex with NAD(P) permit the elucidation of differences between wild-type CgGAPDH and variants in relation to cofactor stabilization.

-	~~Authors~~:	+	Structure-Guided Protein Engineering of Glyceraldehyde-3-phosphate Dehydrogenase from Corynebacterium glutamicum for Dual NAD/NADP Cofactor Specificity.,Son HF, Yu H, Hong J, Lee D, Kim IK, Kim KJ J Agric Food Chem. 2023 Nov 22;71(46):17852-17859. doi: 10.1021/acs.jafc.3c06176. , Epub 2023 Nov 7. PMID:37935620<ref>PMID:37935620</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8hrp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Corynebacterium glutamicum ATCC 13032]]
		+	[[Category: Large Structures]]
		+	[[Category: Kim KJ]]
		+	[[Category: Son HF]]

OCA at 05:47, 25 October 2023

OCA — Wed, 25 Oct 2023 05:47:12 GMT

←Older revision		Revision as of 05:47, 25 October 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8hrp is ON HOLD until ~~Paper Publication~~	+	The entry 8hrp is ON HOLD until 2025-06-15

	Authors:		Authors:

OCA at 10:30, 22 February 2023

OCA — Wed, 22 Feb 2023 10:30:35 GMT

←Older revision		Revision as of 10:30, 22 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8hrp is ON HOLD	+	The entry 8hrp is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8hrp" [edit=sysop:move=sysop]

OCA — Wed, 21 Dec 2022 07:18:18 GMT

Protected "8hrp" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:18, 21 December 2022

OCA: New page: '''Unreleased structure''' The entry 8hrp is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 21 Dec 2022 07:18:14 GMT

New page: '''Unreleased structure''' The entry 8hrp is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8hrp is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8hrp - Revision history

OCA at 08:00, 6 December 2023

OCA at 05:47, 25 October 2023

OCA at 10:30, 22 February 2023

OCA: Protected "8hrp" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8hrp is ON HOLD Authors: Description: Category: Unreleased Structures