OCA at 23:12, 27 December 2023

OCA — Wed, 27 Dec 2023 23:12:09 GMT

←Older revision		Revision as of 23:12, 27 December 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8hvk is ~~ON HOLD until 2024~~-12-27	+	==Crystal structure of SARS-Cov-2 main protease G15S mutant in complex with PF07321332==
-		+	<StructureSection load='8hvk' size='340' side='right'caption='[[8hvk]], [[Resolution\|resolution]] 1.63Å' scene=''>
-	~~Authors~~: ~~Zeng~~, P., ~~Zhang~~, J., Li, J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[8hvk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HVK FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of ~~SARS~~-~~Cov~~-~~2 main protease G15S mutant~~ in ~~complex~~ with ~~PF07321332~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.63Å</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4WI:(1R,2S,5S)-N-{(1E,2S)-1-imino-3-[(3S)-2-oxopyrrolidin-3-yl]propan-2-yl}-6,6-dimethyl-3-[3-methyl-N-(trifluoroacetyl)-L-valyl]-3-azabicyclo[3.1.0]hexane-2-carboxamide'>4WI</scene></td></tr>
-	[[Category: Li, J]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hvk OCA], [https://pdbe.org/8hvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hvk RCSB], [https://www.ebi.ac.uk/pdbsum/8hvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hvk ProSAT]</span></td></tr>
-	[[Category: Zeng, P]]	+	</table>
-	[[Category: Zhang, J]]	+	== Function ==
		+	[https://www.uniprot.org/uniprot/R1A_SARS2 R1A_SARS2] Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.[UniProtKB:P0C6X7] Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.[UniProtKB:P0C6X7] May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.[UniProtKB:P0C6X7] Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.[UniProtKB:P0C6X7] Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.[UniProtKB:P0C6X7] Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).[UniProtKB:P0C6X7] Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] May participate in viral replication by acting as a ssRNA-binding protein.[UniProtKB:P0C6X7] Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.[UniProtKB:P0C6X7]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Li J]]
		+	[[Category: Zeng P]]
		+	[[Category: Zhang J]]

OCA at 08:54, 15 February 2023

OCA — Wed, 15 Feb 2023 08:54:38 GMT

←Older revision		Revision as of 08:54, 15 February 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8hvk is ON HOLD	+	The entry 8hvk is ON HOLD until 2024-12-27

	Authors: Zeng, P., Zhang, J., Li, J.		Authors: Zeng, P., Zhang, J., Li, J.

OCA: Protected "8hvk" [edit=sysop:move=sysop]

OCA — Wed, 11 Jan 2023 06:14:16 GMT

Protected "8hvk" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:14, 11 January 2023

OCA: New page: '''Unreleased structure''' The entry 8hvk is ON HOLD Authors: Zeng, P., Zhang, J., Li, J. Description: Crystal structure of SARS-Cov-2 main protease G15S mutant in complex with PF07321...

OCA — Wed, 11 Jan 2023 06:14:15 GMT

New page: '''Unreleased structure''' The entry 8hvk is ON HOLD Authors: Zeng, P., Zhang, J., Li, J. Description: Crystal structure of SARS-Cov-2 main protease G15S mutant in complex with PF07321...

New page

'''Unreleased structure'''

The entry 8hvk is ON HOLD

Authors: Zeng, P., Zhang, J., Li, J.

Description: Crystal structure of SARS-Cov-2 main protease G15S mutant in complex with PF07321332
[[Category: Unreleased Structures]]
[[Category: Li, J]]
[[Category: Zeng, P]]
[[Category: Zhang, J]]

8hvk - Revision history

OCA at 23:12, 27 December 2023

OCA at 08:54, 15 February 2023

OCA: Protected "8hvk" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8hvk is ON HOLD Authors: Zeng, P., Zhang, J., Li, J. Description: Crystal structure of SARS-Cov-2 main protease G15S mutant in complex with PF07321...