OCA at 12:32, 20 December 2023

OCA — Wed, 20 Dec 2023 12:32:58 GMT

←Older revision		Revision as of 12:32, 20 December 2023
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	</div>		</div>
	<div class="pdbe-citations 8ilm" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 8ilm" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RuBisCO 3D structures\|RuBisCO 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:09, 1 November 2023

OCA — Wed, 01 Nov 2023 13:09:20 GMT

←Older revision		Revision as of 13:09, 1 November 2023
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-	'''Unreleased structure'''

-	The ~~entry~~ 8ilm is ~~ON HOLD until Paper~~ Publication	+	==The cryo-EM structure of eight Rubisco large subunits (RbcL), two Arabidopsis thaliana Rubisco accumulation factors 1 (AtRaf1), and seven Arabidopsis thaliana Bundle Sheath Defective 2 (AtBSD2)==
		+	<StructureSection load='8ilm' size='340' side='right'caption='[[8ilm]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8ilm]] is a 19 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_6301 Synechococcus elongatus PCC 6301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ILM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ILM FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ilm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ilm OCA], [https://pdbe.org/8ilm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ilm RCSB], [https://www.ebi.ac.uk/pdbsum/8ilm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ilm ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RBL_SYNP6 RBL_SYNP6] RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.[HAMAP-Rule:MF_01338]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hexadecameric form I Rubisco, consisting of eight large (RbcL) and eight small (RbcS) subunits, is the most abundant enzyme on earth. Extensive efforts to engineer an improved Rubisco to speed up its catalytic efficiency and ultimately to increase agricultural productivity have been undertaken. However, genetic manipulation of hexadecameric Rubisco has been hampered by its difficulty to fold and assemble correctly in foreign hosts or in vitro. Here we reconstituted Synechococcus sp. PCC6301 Rubisco with the chaperonin system and assembly factors from cyanobacteria and Arabidopsis thaliana (At) in vitro. Rubisco holoenzyme was produced in the presence of either Rubisco accumulation factor 1(Raf1) from cyanobacteria alone, or AtRaf1 and bundle-sheath defective-2 (AtBsd2) from Arabidopsis. RbcL released from GroEL is assembly capable in the presence of ATP and AtBsd2 functions downstream of AtRaf1. Cryo-EM structures of RbcL(8)-AtRaf1(8), RbcL(8)-AtRaf1(4)-AtBsd2(8), and RbcL(8) revealed that the interactions between RbcL-AtRaf1 are looser than those between prokaryotic RbcL-Raf1, with AtRaf1 tilting 7 degrees farther away from RbcL. AtBsd2 stabilizes the flexible regions of RbcL, including the N-, C-terminus, the 60s loop and loop 6. Using these data and combined with previous findings, we propose models for the biogenesis pathways of prokaryotic and eukaryotic Rubisco.

-	~~Authors~~:	+	Structural insight into the functions of Raf1 and Bsd2 in hexadecameric Rubisco assembly.,Wang R, Song H, Zhang W, Wang N, Zhang S, Shao R, Liu C Mol Plant. 2023 Oct 17:S1674-2052(23)00325-8. doi: 10.1016/j.molp.2023.10.011. PMID:37853692<ref>PMID:37853692</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8ilm" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
		+	[[Category: Large Structures]]
		+	[[Category: Synechococcus elongatus PCC 6301]]
		+	[[Category: Shao R]]
		+	[[Category: Song H]]
		+	[[Category: Wang N]]
		+	[[Category: Wang R]]
		+	[[Category: Zhang S]]
		+	[[Category: Zhang W]]

OCA at 07:53, 19 April 2023

OCA — Wed, 19 Apr 2023 07:53:49 GMT

←Older revision		Revision as of 07:53, 19 April 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ilm is ON HOLD	+	The entry 8ilm is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8ilm" [edit=sysop:move=sysop]

OCA — Wed, 08 Mar 2023 06:01:19 GMT

Protected "8ilm" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:01, 8 March 2023

OCA: New page: '''Unreleased structure''' The entry 8ilm is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Mar 2023 06:01:17 GMT

New page: '''Unreleased structure''' The entry 8ilm is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8ilm is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8ilm - Revision history

OCA at 12:32, 20 December 2023

OCA at 13:09, 1 November 2023

OCA at 07:53, 19 April 2023

OCA: Protected "8ilm" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8ilm is ON HOLD Authors: Description: Category: Unreleased Structures