OCA at 08:01, 6 December 2023

OCA — Wed, 06 Dec 2023 08:01:46 GMT

←Older revision		Revision as of 08:01, 6 December 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8jb1 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 in complex with NADP==
		+	<StructureSection load='8jb1' size='340' side='right'caption='[[8jb1]], [[Resolution\|resolution]] 2.44Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8jb1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum_ATCC_13032 Corynebacterium glutamicum ATCC 13032]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JB1 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.44Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jb1 OCA], [https://pdbe.org/8jb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jb1 RCSB], [https://www.ebi.ac.uk/pdbsum/8jb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jb1 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Corynebacterium glutamicum is an industrial workhorse applied in the production of valuable biochemicals. In the process of bio-based chemical production, improving cofactor recycling and mitigating cofactor imbalance are considered major solutions for enhancing the production yield and efficiency. Although, glyceraldehyde-3-phosphate dehydrogenase (GapDH), a glycolytic enzyme, can be a promising candidate for a sufficient NADPH cofactor supply, however, most microorganisms have only NAD-dependent GapDHs. In this study, we performed functional characterization and structure determination of novel NADPH-producing GapDH from C. glutamicum (CgGapX). Based on the crystal structure of CgGapX in complex with NADP cofactor, the unique structural features of CgGapX for NADP stabilization were elucidated. Also, N-terminal additional region (Auxiliary domain, AD) appears to have an effect on enzyme stabilization. In addition, through structure-guided enzyme engineering, we developed a CgGapX variant that exhibited 4.3-fold higher k(cat), and 1.2-fold higher k(cat)/K(M) values when compared with wild-type. Furthermore, a bioinformatic analysis of 100 GapX-like enzymes from 97 microorganisms in the KEGG database revealed that the GapX-like enzymes possess a variety of AD, which seem to determine enzyme stability. Our findings are expected to provide valuable information for supplying NADPH cofactor pools in bio-based value-added chemical production.

-	~~Authors:~~ Son, ~~H.F.~~, Kim, K.J.	+	Structure-based functional analysis of a novel NADPH-producing glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum.,Son HF, Park W, Kim S, Kim IK, Kim KJ Int J Biol Macromol. 2023 Nov 20;255:128103. doi: 10.1016/j.ijbiomac.2023.128103. PMID:37992937<ref>PMID:37992937</ref>

-	~~Description: Crystal structure~~ of ~~glyceraldehyde~~-3-~~phosphate dehydrogenase from~~ Corynebacterium glutamicum ~~ATCC13032 in complex with NADP~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Son, H.F~~]]	+	<div class="pdbe-citations 8jb1" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Kim, K.J~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Corynebacterium glutamicum ATCC 13032]]
		+	[[Category: Large Structures]]
		+	[[Category: Kim KJ]]
		+	[[Category: Son HF]]

OCA at 03:58, 31 May 2023

OCA — Wed, 31 May 2023 03:58:36 GMT

←Older revision		Revision as of 03:58, 31 May 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8jb1 is ON HOLD	+	The entry 8jb1 is ON HOLD until Paper Publication

	Authors: Son, H.F., Kim, K.J.		Authors: Son, H.F., Kim, K.J.

OCA: Protected "8jb1" [edit=sysop:move=sysop]

OCA — Thu, 18 May 2023 05:32:31 GMT

Protected "8jb1" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:32, 18 May 2023

OCA: New page: '''Unreleased structure''' The entry 8jb1 is ON HOLD Authors: Son, H.F., Kim, K.J. Description: Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium gluta...

OCA — Thu, 18 May 2023 05:32:30 GMT

New page: '''Unreleased structure''' The entry 8jb1 is ON HOLD Authors: Son, H.F., Kim, K.J. Description: Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium gluta...

New page

'''Unreleased structure'''

The entry 8jb1 is ON HOLD

Authors: Son, H.F., Kim, K.J.

Description: Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 in complex with NADP
[[Category: Unreleased Structures]]
[[Category: Son, H.F]]
[[Category: Kim, K.J]]

8jb1 - Revision history

OCA at 08:01, 6 December 2023

OCA at 03:58, 31 May 2023

OCA: Protected "8jb1" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8jb1 is ON HOLD Authors: Son, H.F., Kim, K.J. Description: Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium gluta...