OCA at 09:38, 1 March 2024

OCA — Fri, 01 Mar 2024 09:38:11 GMT

←Older revision		Revision as of 09:38, 1 March 2024
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8jg9 is ~~ON HOLD until Paper Publication~~	+	==Cryo-EM structure of the SaCas9-sgRNA-AcrIIA15-promoter DNA dimer==
-		+	<StructureSection load='8jg9' size='340' side='right'caption='[[8jg9]], [[Resolution\|resolution]] 3.82Å' scene=''>
-	~~Authors~~: ~~Deng~~, X., ~~Wang~~, Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[8jg9]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] and [https://en.wikipedia.org/wiki/Staphylococcus_delphini Staphylococcus delphini]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JG9 FirstGlance]. <br>
-	~~Description~~: ~~Cryo-EM structure~~ of ~~the SaCas9~~-~~sgRNA~~-~~AcrIIA15~~-~~promoter DNA dimer~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.82Å</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jg9 OCA], [https://pdbe.org/8jg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jg9 RCSB], [https://www.ebi.ac.uk/pdbsum/8jg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jg9 ProSAT]</span></td></tr>
-	[[Category: Deng, X]]	+	</table>
-	[[Category: Wang, Y]]	+	== Function ==
		+	[https://www.uniprot.org/uniprot/CAS9_STAAU CAS9_STAAU] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.[HAMAP-Rule:MF_01480]<ref>PMID:25830891</ref> <ref>PMID:26098369</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Staphylococcus aureus]]
		+	[[Category: Staphylococcus delphini]]
		+	[[Category: Deng X]]
		+	[[Category: Wang Y]]

OCA at 19:40, 28 June 2023

OCA — Wed, 28 Jun 2023 19:40:17 GMT

←Older revision		Revision as of 19:40, 28 June 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8jg9 is ON HOLD	+	The entry 8jg9 is ON HOLD until Paper Publication

	Authors: Deng, X., Wang, Y.		Authors: Deng, X., Wang, Y.

OCA: Protected "8jg9" [edit=sysop:move=sysop]

OCA — Wed, 31 May 2023 04:00:03 GMT

Protected "8jg9" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:00, 31 May 2023

OCA: New page: '''Unreleased structure''' The entry 8jg9 is ON HOLD Authors: Deng, X., Wang, Y. Description: Cryo-EM structure of the SaCas9-sgRNA-AcrIIA15-promoter DNA dimer [[Category: Unreleased S...

OCA — Wed, 31 May 2023 04:00:01 GMT

New page: '''Unreleased structure''' The entry 8jg9 is ON HOLD Authors: Deng, X., Wang, Y. Description: Cryo-EM structure of the SaCas9-sgRNA-AcrIIA15-promoter DNA dimer [[Category: Unreleased S...

New page

'''Unreleased structure'''

The entry 8jg9 is ON HOLD

Authors: Deng, X., Wang, Y.

Description: Cryo-EM structure of the SaCas9-sgRNA-AcrIIA15-promoter DNA dimer
[[Category: Unreleased Structures]]
[[Category: Deng, X]]
[[Category: Wang, Y]]

8jg9 - Revision history

OCA at 09:38, 1 March 2024

OCA at 19:40, 28 June 2023

OCA: Protected "8jg9" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8jg9 is ON HOLD Authors: Deng, X., Wang, Y. Description: Cryo-EM structure of the SaCas9-sgRNA-AcrIIA15-promoter DNA dimer [[Category: Unreleased S...