OCA at 12:35, 4 October 2023

OCA — Wed, 04 Oct 2023 12:35:29 GMT

←Older revision		Revision as of 12:35, 4 October 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8jhe is ~~ON HOLD until Paper~~ Publication	+	==Hyper-thermostable ancestral L-amino acid oxidase 2 (HTAncLAAO2)==
		+	<StructureSection load='8jhe' size='340' side='right'caption='[[8jhe]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8jhe]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JHE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JHE FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.201Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jhe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jhe OCA], [https://pdbe.org/8jhe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jhe RCSB], [https://www.ebi.ac.uk/pdbsum/8jhe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jhe ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Production of D-amino acids (D-AAs) on a large-scale enables to provide precursors of peptide therapeutics. In this study, we designed a novel L-amino acid oxidase, HTAncLAAO2, by ancestral sequence reconstruction, exhibiting high thermostability and long-term stability. The crystal structure of HTAncLAAO2 was determined at 2.2 A by X-ray crystallography, revealing that the enzyme has an octameric form like a "ninja-star" feature. Enzymatic property analysis demonstrated that HTAncLAAO2 exhibits three-order larger k(cat)/K(m) values towards four L-AAs (L-Phe, L-Leu, L-Met, and L-Ile) than that of L-Trp. Through screening the variants, we obtained the HTAncLAAO2(W220A) variant, which shows a > 6-fold increase in k(cat) value toward L-Trp compared to the original enzyme. This variant applies to synthesizing enantio-pure D-Trp derivatives from L- or rac-forms at a preparative scale. Given its excellent properties, HTAncLAAO2 would be a starting point for designing novel oxidases with high activity toward various amines and AAs.

-	~~Authors: Kawamura~~, Y., Ishida, C., Miyata, R., Miyata, A., Hayashi, S., Fujinami, D., Ito, S., Nakano, S.	+	Structural and functional analysis of hyper-thermostable ancestral L-amino acid oxidase that can convert Trp derivatives to D-forms by chemoenzymatic reaction.,Kawamura Y, Ishida C, Miyata R, Miyata A, Hayashi S, Fujinami D, Ito S, Nakano S Commun Chem. 2023 Sep 22;6(1):200. doi: 10.1038/s42004-023-01005-1. PMID:37737277<ref>PMID:37737277</ref>

-	~~Description: Hyper~~-~~thermostable ancestral L~~-~~amino acid oxidase 2 (HTAncLAAO2)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Kawamura, Y~~]]	+	<div class="pdbe-citations 8jhe" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Ito, S~~]]	+	== References ==
-	[[Category: ~~Nakano,~~ S]]	+	<references/>
-	[[Category: ~~Miyata, A~~]]	+	__TOC__
-	[[Category: ~~Fujinami, D~~]]	+	</StructureSection>
-	[[Category: ~~Ishida, C~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Hayashi, S~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Miyata, R]]	+	[[Category: Fujinami D]]
		+	[[Category: Hayashi S]]
		+	[[Category: Ishida C]]
		+	[[Category: Ito S]]
		+	[[Category: Kawamura Y]]
		+	[[Category: Miyata A]]
		+	[[Category: Miyata R]]
		+	[[Category: Nakano S]]

OCA at 03:55, 5 July 2023

OCA — Wed, 05 Jul 2023 03:55:54 GMT

←Older revision		Revision as of 03:55, 5 July 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8jhe is ON HOLD	+	The entry 8jhe is ON HOLD until Paper Publication

	Authors: Kawamura, Y., Ishida, C., Miyata, R., Miyata, A., Hayashi, S., Fujinami, D., Ito, S., Nakano, S.		Authors: Kawamura, Y., Ishida, C., Miyata, R., Miyata, A., Hayashi, S., Fujinami, D., Ito, S., Nakano, S.

OCA: Protected "8jhe" [edit=sysop:move=sysop]

OCA — Wed, 31 May 2023 04:02:34 GMT

Protected "8jhe" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:02, 31 May 2023

OCA: New page: '''Unreleased structure''' The entry 8jhe is ON HOLD Authors: Kawamura, Y., Ishida, C., Miyata, R., Miyata, A., Hayashi, S., Fujinami, D., Ito, S., Nakano, S. Description: Hyper-thermo...

OCA — Wed, 31 May 2023 04:02:33 GMT

New page: '''Unreleased structure''' The entry 8jhe is ON HOLD Authors: Kawamura, Y., Ishida, C., Miyata, R., Miyata, A., Hayashi, S., Fujinami, D., Ito, S., Nakano, S. Description: Hyper-thermo...

New page

'''Unreleased structure'''

The entry 8jhe is ON HOLD

Authors: Kawamura, Y., Ishida, C., Miyata, R., Miyata, A., Hayashi, S., Fujinami, D., Ito, S., Nakano, S.

Description: Hyper-thermostable ancestral L-amino acid oxidase 2 (HTAncLAAO2)
[[Category: Unreleased Structures]]
[[Category: Kawamura, Y]]
[[Category: Ito, S]]
[[Category: Nakano, S]]
[[Category: Miyata, A]]
[[Category: Fujinami, D]]
[[Category: Ishida, C]]
[[Category: Hayashi, S]]
[[Category: Miyata, R]]

8jhe - Revision history

OCA at 12:35, 4 October 2023

OCA at 03:55, 5 July 2023

OCA: Protected "8jhe" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8jhe is ON HOLD Authors: Kawamura, Y., Ishida, C., Miyata, R., Miyata, A., Hayashi, S., Fujinami, D., Ito, S., Nakano, S. Description: Hyper-thermo...