OCA at 12:35, 4 October 2023

OCA — Wed, 04 Oct 2023 12:35:49 GMT

←Older revision		Revision as of 12:35, 4 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8jld is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of the 145 bp human nucleosome containing acetylated H3 tail==
		+	<StructureSection load='8jld' size='340' side='right'caption='[[8jld]], [[Resolution\|resolution]] 2.48Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8jld]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JLD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JLD FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.48Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jld OCA], [https://pdbe.org/8jld PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jld RCSB], [https://www.ebi.ac.uk/pdbsum/8jld PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jld ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/H4_HUMAN H4_HUMAN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The N-terminal tails of histones protrude from the nucleosome core and are target sites for histone modifications, such as acetylation and methylation. Histone acetylation is considered to enhance transcription in chromatin. However, the contribution of the histone N-terminal tail to the nucleosome transcription by RNA polymerase II (RNAPII) has not been clarified. In the present study, we reconstituted nucleosomes lacking the N-terminal tail of each histone, H2A, H2B, H3 or H4, and performed RNAPII transcription assays. We found that the N-terminal tail of H3, but not H2A, H2B and H4, functions in RNAPII pausing at the SHL(-5) position of the nucleosome. Consistently, the RNAPII transcription assay also revealed that the nucleosome containing N-terminally acetylated H3 drastically alleviates RNAPII pausing at the SHL(-5) position. In addition, the H3 acetylated nucleosome produced increased amounts of the run-off transcript. These results provide important evidence that the H3 N-terminal tail plays a role in RNAPII pausing at the SHL(-5) position of the nucleosome, and its acetylation directly alleviates this nucleosome barrier.

-	~~Authors~~:	+	Contributions of histone tail clipping and acetylation in nucleosome transcription by RNA polymerase II.,Oishi T, Hatazawa S, Kujirai T, Kato J, Kobayashi Y, Ogasawara M, Akatsu M, Ehara H, Sekine SI, Hayashi G, Takizawa Y, Kurumizaka H Nucleic Acids Res. 2023 Sep 18:gkad754. doi: 10.1093/nar/gkad754. PMID:37718728<ref>PMID:37718728</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8jld" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Akatsu M]]
		+	[[Category: Hatazawa S]]
		+	[[Category: Kato J]]
		+	[[Category: Kobayashi Y]]
		+	[[Category: Kujirai T]]
		+	[[Category: Kurumizaka H]]
		+	[[Category: Ogasawara M]]
		+	[[Category: Oishi T]]
		+	[[Category: Takizawa Y]]

OCA at 03:55, 12 July 2023

OCA — Wed, 12 Jul 2023 03:55:13 GMT

←Older revision		Revision as of 03:55, 12 July 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8jld is ON HOLD	+	The entry 8jld is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8jld" [edit=sysop:move=sysop]

OCA — Wed, 07 Jun 2023 04:19:17 GMT

Protected "8jld" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:19, 7 June 2023

OCA: New page: '''Unreleased structure''' The entry 8jld is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Jun 2023 04:19:16 GMT

New page: '''Unreleased structure''' The entry 8jld is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8jld is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8jld - Revision history

OCA at 12:35, 4 October 2023

OCA at 03:55, 12 July 2023

OCA: Protected "8jld" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8jld is ON HOLD Authors: Description: Category: Unreleased Structures