OCA at 11:43, 1 February 2024

OCA — Thu, 01 Feb 2024 11:43:03 GMT

←Older revision		Revision as of 11:43, 1 February 2024
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8jpx is ~~ON HOLD until Paper Publication~~	+	==Cryo-EM structure of PfAgo-guide DNA-target DNA complex==
-		+	<StructureSection load='8jpx' size='340' side='right'caption='[[8jpx]], [[Resolution\|resolution]] 2.90Å' scene=''>
-	~~Authors~~: ~~Zhuang~~, L.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[8jpx]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JPX FirstGlance]. <br>
-	~~Description~~: ~~Cryo~~-~~EM structure~~ of ~~an RNP~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: Zhuang, L]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jpx OCA], [https://pdbe.org/8jpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jpx RCSB], [https://www.ebi.ac.uk/pdbsum/8jpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jpx ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AGO_PYRFU AGO_PYRFU] A DNA-guided ssDNA endonuclease that may play a role in defense against invading mobile genetic elements. Uses short 5'-phospho-ssDNA sequences as guides (gDNA) to bind complementary target strands, resulting in cleavage of the target DNA (tDNA). Endonucleolytically cleaves DNA in short dsDNA (the gDNA indicates where to cleave on the tDNA). Efficient guide-dependent target DNA cleavage requires a minimal gDNA length of 15 nucleotides (nt) and works up to at least 31 nt. Overexpression decreases plasmid transformation efficiency. Has no appreciable activity with gRNA or on target RNA. Also has guide-independent activity on plasmid DNA called 'chopping' (PubMed:25925567). The cleavage site is 10 nucleotides (nt) downstream of the target residue base-paired with the 5'-end of the gDNA, cleavage is insensitive to adenine methylation. DNA cleavage produces 5'-phosphomonoesters (as it can be ligated by T4 DNA ligase) (PubMed:28165224).<ref>PMID:25925567</ref> <ref>PMID:28165224</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pyrococcus furiosus DSM 3638]]
		+	[[Category: Zhuang L]]

OCA at 10:43, 26 July 2023

OCA — Wed, 26 Jul 2023 10:43:09 GMT

←Older revision		Revision as of 10:43, 26 July 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8jpx is ON HOLD	+	The entry 8jpx is ON HOLD until Paper Publication

	Authors: Zhuang, L.		Authors: Zhuang, L.

OCA: Protected "8jpx" [edit=sysop:move=sysop]

OCA — Wed, 21 Jun 2023 08:06:38 GMT

Protected "8jpx" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:06, 21 June 2023

OCA: New page: '''Unreleased structure''' The entry 8jpx is ON HOLD Authors: Zhuang, L. Description: Cryo-EM structure of an RNP Category: Unreleased Structures Category: Zhuang, L

OCA — Wed, 21 Jun 2023 08:06:37 GMT

New page: '''Unreleased structure''' The entry 8jpx is ON HOLD Authors: Zhuang, L. Description: Cryo-EM structure of an RNP Category: Unreleased Structures Category: Zhuang, L

New page

'''Unreleased structure'''

The entry 8jpx is ON HOLD

Authors: Zhuang, L.

Description: Cryo-EM structure of an RNP
[[Category: Unreleased Structures]]
[[Category: Zhuang, L]]

8jpx - Revision history

OCA at 11:43, 1 February 2024

OCA at 10:43, 26 July 2023

OCA: Protected "8jpx" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8jpx is ON HOLD Authors: Zhuang, L. Description: Cryo-EM structure of an RNP Category: Unreleased Structures Category: Zhuang, L