OCA at 11:27, 24 January 2024

OCA — Wed, 24 Jan 2024 11:27:40 GMT

←Older revision		Revision as of 11:27, 24 January 2024
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8jsh is ~~ON HOLD until Paper~~ Publication	+	==Structure of the 30S-body-IF3 complex from Escherichia coli==
		+	<StructureSection load='8jsh' size='340' side='right'caption='[[8jsh]], [[Resolution\|resolution]] 4.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8jsh]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JSH FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jsh OCA], [https://pdbe.org/8jsh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jsh RCSB], [https://www.ebi.ac.uk/pdbsum/8jsh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jsh ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RS18_ECOLI RS18_ECOLI] Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit.[HAMAP-Rule:MF_00270]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial ribosomes require three initiation factors IF1, IF2, and IF3 during the initial steps of translation. These IFs ensure correct base pairing of the initiator tRNA anticodon with the start codon in the mRNA located at the P-site of the 30S ribosomal subunit. IF3 is one of the first IFs to bind to the 30S and plays a crucial role in the selection of the correct start codon and codon: anticodon base pairing. IF3 also prevents the premature association of the 50S subunit of ribosomes and aids in ribosome recycling. IF3 is reported to change binding sites and conformation to ensure translation initiation fidelity. A recent study suggested an initial binding of IF3 CTD away from the P-site and that IF1 and IF2 promote the movement of CTD to the P-site and concomitant movement of NTD. Hence, to visualize the position of IF3 in the absence of any other IFs, we determined cryo-EM structure of the 30S-IF3 complex. The map shows that IF3 is present in an extended conformation with CTD present at the P-site and NTD near the platform even in the absence of IF1 and IF2. Hence, IF3 CTD binds at the P-site and moves away during the accommodation of the initiator tRNA at the P-site in the later steps of translation initiation. Overall, we report the structure of 30S-IF3 which demystifies the starting binding site and conformation of IF3 on the 30S ribosomal subunit.

-	~~Authors: Uday, A.B~~., Mishra~~, R.K.~~, Hussain, T.	+	Initiation factor 3 bound to the 30S ribosomal subunit in an initial step of translation.,Uday AB, Mishra RK, Hussain T Proteins. 2023 Dec 26. doi: 10.1002/prot.26655. PMID:38148682<ref>PMID:38148682</ref>

-	~~Description: Structure~~ of the ~~30S~~-~~body~~-~~IF3 complex from~~ Escherichia coli	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Hussain, T]]	+	<div class="pdbe-citations 8jsh" style="background-color:#fffaf0;"></div>
-	[[Category: Mishra~~, R.K~~]]	+	== References ==
-	[[Category: Uday~~, A.B~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Hussain T]]
		+	[[Category: Mishra RK]]
		+	[[Category: Uday AB]]

OCA at 07:09, 30 August 2023

OCA — Wed, 30 Aug 2023 07:09:21 GMT

←Older revision		Revision as of 07:09, 30 August 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8jsh is ON HOLD	+	The entry 8jsh is ON HOLD until Paper Publication

	Authors: Uday, A.B., Mishra, R.K., Hussain, T.		Authors: Uday, A.B., Mishra, R.K., Hussain, T.

OCA: Protected "8jsh" [edit=sysop:move=sysop]

OCA — Wed, 28 Jun 2023 19:44:14 GMT

Protected "8jsh" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:44, 28 June 2023

OCA: New page: '''Unreleased structure''' The entry 8jsh is ON HOLD Authors: Uday, A.B., Mishra, R.K., Hussain, T. Description: Structure of the 30S-body-IF3 complex from Escherichia coli [[Category:...

OCA — Wed, 28 Jun 2023 19:44:13 GMT

New page: '''Unreleased structure''' The entry 8jsh is ON HOLD Authors: Uday, A.B., Mishra, R.K., Hussain, T. Description: Structure of the 30S-body-IF3 complex from Escherichia coli [[Category:...

New page

'''Unreleased structure'''

The entry 8jsh is ON HOLD

Authors: Uday, A.B., Mishra, R.K., Hussain, T.

Description: Structure of the 30S-body-IF3 complex from Escherichia coli
[[Category: Unreleased Structures]]
[[Category: Hussain, T]]
[[Category: Mishra, R.K]]
[[Category: Uday, A.B]]

8jsh - Revision history

OCA at 11:27, 24 January 2024

OCA at 07:09, 30 August 2023

OCA: Protected "8jsh" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8jsh is ON HOLD Authors: Uday, A.B., Mishra, R.K., Hussain, T. Description: Structure of the 30S-body-IF3 complex from Escherichia coli [[Category:...