OCA at 05:59, 11 October 2023

OCA — Wed, 11 Oct 2023 05:59:19 GMT

←Older revision		Revision as of 05:59, 11 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8jz0 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of a single-chain monellin mutant C41A==
		+	<StructureSection load='8jz0' size='340' side='right'caption='[[8jz0]], [[Resolution\|resolution]] 1.23Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8jz0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JZ0 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.229Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jz0 OCA], [https://pdbe.org/8jz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jz0 RCSB], [https://www.ebi.ac.uk/pdbsum/8jz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jz0 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU] Taste-modifying protein; intensely sweet-tasting protein.[https://www.uniprot.org/uniprot/MONA_DIOCU MONA_DIOCU] Taste-modifying protein; intensely sweet-tasting protein.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Single-chain monellin (SCM) is an engineered protein that links the two chains of monellin, a naturally sweet-tasting protein. This protein is an attractive candidate for use as a sugar replacement in food and beverages and has numerous other applications. Therefore, generating SCM mutants with improved stability is an active area of research to broaden the range of its potential applications. In this study, we focused on the Cys41 residue of SCM, which is a single cysteine residue present at a structurally important position. This residue is often substituted with Ser. However, this substitution may destabilize SCM because Cys41 is buried in the hydrophobic core of the protein. Therefore, we designed mutants that substituted Ala, Val, and Leu for this residue, namely C41A, C41V, and C41L. We characterized these three mutants, SCM C41S, and wild type (WT). Differential scanning fluorimetric analysis revealed that substituting Cys41 with Ala or Val increased the thermal stability of SCM, while substitution with Ser or Leu decreased its stability. Determination of the crystal structures of SCM C41A and C41V mutants revealed that the overall structures and main chain structures around the 41st residue of both mutants were almost identical to the WT. On the other hand, the orientations of the amino acid side chains near the 41st residue differed among the SCM variants. Taken together, our results indicate that substituting Cys41 with Ala or Val increases the stability of SCM and provide insight into the structural basis of this improvement.

-	~~Authors~~:	+	Investigating the Effect of Substituting a Single Cysteine Residue on the Thermal Stability of an Engineered Sweet Protein, Single-Chain Monellin.,Ohnuma K, Yamashita A, Yasui N Protein J. 2023 Sep 22. doi: 10.1007/s10930-023-10154-0. PMID:37737932<ref>PMID:37737932</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8jz0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Dioscoreophyllum cumminsii]]
		+	[[Category: Large Structures]]
		+	[[Category: Ohnuma K]]
		+	[[Category: Yamashita A]]
		+	[[Category: Yasui N]]

OCA at 04:40, 19 July 2023

OCA — Wed, 19 Jul 2023 04:40:52 GMT

←Older revision		Revision as of 04:40, 19 July 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8jz0 is ON HOLD	+	The entry 8jz0 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8jz0" [edit=sysop:move=sysop]

OCA — Wed, 12 Jul 2023 04:05:11 GMT

Protected "8jz0" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:05, 12 July 2023

OCA: New page: '''Unreleased structure''' The entry 8jz0 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 12 Jul 2023 04:05:09 GMT

New page: '''Unreleased structure''' The entry 8jz0 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8jz0 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8jz0 - Revision history

OCA at 05:59, 11 October 2023

OCA at 04:40, 19 July 2023

OCA: Protected "8jz0" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8jz0 is ON HOLD Authors: Description: Category: Unreleased Structures