OCA at 07:06, 25 October 2023

OCA — Wed, 25 Oct 2023 07:06:25 GMT

←Older revision		Revision as of 07:06, 25 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8jzh is ~~ON HOLD~~	+	==C. glutamicum S-adenosylmethionine synthase==
		+	<StructureSection load='8jzh' size='340' side='right'caption='[[8jzh]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8jzh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum_ATCC_13032 Corynebacterium glutamicum ATCC 13032]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JZH FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jzh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jzh OCA], [https://pdbe.org/8jzh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jzh RCSB], [https://www.ebi.ac.uk/pdbsum/8jzh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jzh ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/METK_CORGL METK_CORGL] Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.[HAMAP-Rule:MF_00086]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	S-Adenosylmethionine (SAM) acts as a methyl donor in living organisms, and S-adenosylmethionine synthetase (MetK) is an essential enzyme for cells, as it synthesizes SAM from methionine and adenosine triphosphate (ATP). This study determined the crystal structures of the apo form and adenosine/triphosphate complex form of MetK from Corynebacterium glutamicum (CgMetK). Results showed that CgMetK has an allosteric inhibitor binding site for the SAM product in the vicinity of the active site and is inhibited by SAM both competitively and noncompetitively. Through structure-guided protein engineering, the CgMetK(E68A) variant was developed that exhibited an almost complete release of inhibition by SAM with rather enhanced enzyme activity. The crystal structure of the CgMetK(E68A) variant revealed that the formation of a new hydrogen bond between Tyr66 and Glu102 by the E68A mutation disrupted the allosteric SAM binding site and also improved the protein thermal stability by strengthening the tetramerization of the enzyme.

-	~~Authors~~:	+	Structural and Biochemical Studies on Product Inhibition of S-Adenosylmethionine Synthetase from Corynebacterium glutamicum.,Lee S, Kim S, Kim IK, Kim KJ J Agric Food Chem. 2023 Oct 16. doi: 10.1021/acs.jafc.3c05180. PMID:37846083<ref>PMID:37846083</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8jzh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Corynebacterium glutamicum ATCC 13032]]
		+	[[Category: Large Structures]]
		+	[[Category: Kim KJ]]
		+	[[Category: Lee S]]

OCA at 04:46, 27 September 2023

OCA — Wed, 27 Sep 2023 04:46:09 GMT

←Older revision		Revision as of 04:46, 27 September 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8jzh is ON HOLD ~~until Paper Publication~~	+	The entry 8jzh is ON HOLD

	Authors:		Authors:

OCA at 08:55, 2 August 2023

OCA — Wed, 02 Aug 2023 08:55:11 GMT

←Older revision		Revision as of 08:55, 2 August 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8jzh is ON HOLD	+	The entry 8jzh is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8jzh" [edit=sysop:move=sysop]

OCA — Wed, 12 Jul 2023 04:06:51 GMT

Protected "8jzh" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:06, 12 July 2023

OCA: New page: '''Unreleased structure''' The entry 8jzh is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 12 Jul 2023 04:06:49 GMT

New page: '''Unreleased structure''' The entry 8jzh is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8jzh is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8jzh - Revision history

OCA at 07:06, 25 October 2023

OCA at 04:46, 27 September 2023

OCA at 08:55, 2 August 2023

OCA: Protected "8jzh" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8jzh is ON HOLD Authors: Description: Category: Unreleased Structures