OCA at 05:59, 11 October 2023

OCA — Wed, 11 Oct 2023 05:59:26 GMT

←Older revision		Revision as of 05:59, 11 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8kgk is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of the GPR61-Gs complex==
		+	<StructureSection load='8kgk' size='340' side='right'caption='[[8kgk]], [[Resolution\|resolution]] 3.16Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8kgk]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8KGK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8KGK FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.16Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8kgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8kgk OCA], [https://pdbe.org/8kgk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8kgk RCSB], [https://www.ebi.ac.uk/pdbsum/8kgk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8kgk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GBB1_HUMAN GBB1_HUMAN] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.<ref>PMID:18611381</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many orphan G protein-coupled receptors (GPCRs) remain understudied because their endogenous ligands are unknown. Here, we show that a group of class A/rhodopsin-like orphan GPCRs including GPR61, GPR161 and GPR174 increase the cAMP level similarly to fully activated D1 dopamine receptor (D1R). We report cryo-electron microscopy structures of the GPR61âG(s), GPR161âG(s) and GPR174âG(s) complexes without any exogenous ligands. The GPR174 structure reveals that endogenous lysophosphatidylserine (lysoPS) is copurified. While GPR174 fails to respond to exogenous lysoPS, likely owing to its maximal activation by the endogenous ligand, GPR174 mutants with lower ligand binding affinities can be specifically activated by lysoPS but not other lipids, in a dose-dependent manner. Moreover, GPR174 adopts a non-canonical G(s) coupling mode. The structures of GPR161 and GPR61 reveal that the second extracellular loop (ECL2) penetrates into the orthosteric pocket, possibly contributing to constitutive activity. Our work definitively confirms lysoPS as an endogenous GPR174 ligand and suggests that high constitutive activity of some orphan GPCRs could be accounted for by their having naturally abundant ligands.

-	~~Authors~~:	+	Specific binding of GPR174 by endogenous lysophosphatidylserine leads to high constitutive G(s) signaling.,Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S Nat Commun. 2023 Sep 22;14(1):5901. doi: 10.1038/s41467-023-41654-3. PMID:37737235<ref>PMID:37737235</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8kgk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Lama glama]]
		+	[[Category: Large Structures]]
		+	[[Category: Nie Y]]
		+	[[Category: Qiu Z]]
		+	[[Category: Zheng S]]

OCA at 04:20, 6 September 2023

OCA — Wed, 06 Sep 2023 04:20:17 GMT

←Older revision		Revision as of 04:20, 6 September 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8kgk is ON HOLD	+	The entry 8kgk is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8kgk" [edit=sysop:move=sysop]

OCA — Wed, 30 Aug 2023 07:14:52 GMT

Protected "8kgk" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:14, 30 August 2023

OCA: New page: '''Unreleased structure''' The entry 8kgk is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 30 Aug 2023 07:14:51 GMT

New page: '''Unreleased structure''' The entry 8kgk is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8kgk is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8kgk - Revision history

OCA at 05:59, 11 October 2023

OCA at 04:20, 6 September 2023

OCA: Protected "8kgk" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8kgk is ON HOLD Authors: Description: Category: Unreleased Structures