8oh9 - Revision history

OCA at 11:20, 14 March 2024

OCA — Thu, 14 Mar 2024 11:20:57 GMT

←Older revision		Revision as of 11:20, 14 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A0U1KYM9_9FIRM A0A0U1KYM9_9FIRM]		[https://www.uniprot.org/uniprot/A0A0U1KYM9_9FIRM A0A0U1KYM9_9FIRM]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The challenge of endergonic reduction of NADP(+) using NADH is overcome by ferredoxin-dependent transhydrogenases that employ electron bifurcation for electron carrier adjustments in the ancient Wood-Ljungdahl pathway. Recently, an electron-bifurcating transhydrogenase with subunit compositions distinct from the well-characterized Nfn-type transhydrogenase was described: the Stn complex. Here, we present the single-particle cryo-EM structure of the Stn family transhydrogenase from the acetogenic bacterium Sporomusa ovata and functionally dissect its electron transfer pathway. Stn forms a tetramer consisting of functional heterotrimeric StnABC complexes. Our findings demonstrate that the StnAB subunits assume the structural and functional role of a bifurcating module, homologous to the HydBC core of the electron-bifurcating HydABC complex. Moreover, StnC contains a NuoG-like domain and a GltD-like NADPH binding domain that resembles the NfnB subunit of the NfnAB complex. However, in contrast to NfnB, StnC lost the ability to bifurcate electrons. Structural comparison allows us to describe how the same fold on one hand evolved bifurcation activity on its own while on the other hand combined with an associated bifurcating module, exemplifying modular evolution in anaerobic metabolism to produce activities critical for survival at the thermodynamic limit of life.
-
-	Molecular architecture and electron transfer pathway of the Stn family transhydrogenase.,Kumar A, Kremp F, Roth J, Freibert SA, Muller V, Schuller JM Nat Commun. 2023 Sep 7;14(1):5484. doi: 10.1038/s41467-023-41212-x. PMID:37673911<ref>PMID:37673911</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8oh9" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 18:18, 20 September 2023

OCA — Wed, 20 Sep 2023 18:18:31 GMT

←Older revision		Revision as of 18:18, 20 September 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8oh9 is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of the electron bifurcating transhydrogenase StnABC complex from Sporomusa Ovata (state 1)==
		+	<StructureSection load='8oh9' size='340' side='right'caption='[[8oh9]], [[Resolution\|resolution]] 3.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8oh9]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Sporomusa_ovata_DSM_2662 Sporomusa ovata DSM 2662]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OH9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OH9 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8oh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8oh9 OCA], [https://pdbe.org/8oh9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8oh9 RCSB], [https://www.ebi.ac.uk/pdbsum/8oh9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8oh9 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0U1KYM9_9FIRM A0A0U1KYM9_9FIRM]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The challenge of endergonic reduction of NADP(+) using NADH is overcome by ferredoxin-dependent transhydrogenases that employ electron bifurcation for electron carrier adjustments in the ancient Wood-Ljungdahl pathway. Recently, an electron-bifurcating transhydrogenase with subunit compositions distinct from the well-characterized Nfn-type transhydrogenase was described: the Stn complex. Here, we present the single-particle cryo-EM structure of the Stn family transhydrogenase from the acetogenic bacterium Sporomusa ovata and functionally dissect its electron transfer pathway. Stn forms a tetramer consisting of functional heterotrimeric StnABC complexes. Our findings demonstrate that the StnAB subunits assume the structural and functional role of a bifurcating module, homologous to the HydBC core of the electron-bifurcating HydABC complex. Moreover, StnC contains a NuoG-like domain and a GltD-like NADPH binding domain that resembles the NfnB subunit of the NfnAB complex. However, in contrast to NfnB, StnC lost the ability to bifurcate electrons. Structural comparison allows us to describe how the same fold on one hand evolved bifurcation activity on its own while on the other hand combined with an associated bifurcating module, exemplifying modular evolution in anaerobic metabolism to produce activities critical for survival at the thermodynamic limit of life.

-	~~Authors~~:	+	Molecular architecture and electron transfer pathway of the Stn family transhydrogenase.,Kumar A, Kremp F, Roth J, Freibert SA, Muller V, Schuller JM Nat Commun. 2023 Sep 7;14(1):5484. doi: 10.1038/s41467-023-41212-x. PMID:37673911<ref>PMID:37673911</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8oh9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Sporomusa ovata DSM 2662]]
		+	[[Category: Kremp F]]
		+	[[Category: Kumar A]]
		+	[[Category: Mueller V]]
		+	[[Category: Schuller JM]]

OCA at 09:00, 2 August 2023

OCA — Wed, 02 Aug 2023 09:00:57 GMT

←Older revision		Revision as of 09:00, 2 August 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8oh9 is ON HOLD	+	The entry 8oh9 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 04:07, 5 July 2023

OCA — Wed, 05 Jul 2023 04:07:34 GMT

←Older revision		Revision as of 04:07, 5 July 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8oh9 is ON HOLD ~~until Paper Publication~~	+	The entry 8oh9 is ON HOLD

	Authors:		Authors:

OCA at 15:10, 26 April 2023

OCA — Wed, 26 Apr 2023 15:10:18 GMT

←Older revision		Revision as of 15:10, 26 April 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8oh9 is ON HOLD	+	The entry 8oh9 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8oh9" [edit=sysop:move=sysop]

OCA — Wed, 29 Mar 2023 05:06:48 GMT

Protected "8oh9" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:06, 29 March 2023

OCA: New page: '''Unreleased structure''' The entry 8oh9 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Mar 2023 05:06:47 GMT

New page: '''Unreleased structure''' The entry 8oh9 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8oh9 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]