OCA at 10:39, 2 August 2023

OCA — Wed, 02 Aug 2023 10:39:54 GMT

←Older revision		Revision as of 10:39, 2 August 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8oia is ~~ON HOLD until Paper~~ Publication	+	==Trichomonas vaginalis riboside hydrolase in complex with D-ribose==
		+	<StructureSection load='8oia' size='340' side='right'caption='[[8oia]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8oia]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichomonas_vaginalis Trichomonas vaginalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OIA FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=RIB:RIBOSE'>RIB</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8oia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8oia OCA], [https://pdbe.org/8oia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8oia RCSB], [https://www.ebi.ac.uk/pdbsum/8oia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8oia ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A2FTT0_TRIV3 A2FTT0_TRIV3]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pathogenic parasites of the Trichomonas genus are causative agents of sexually-transmitted diseases affecting millions of individuals worldwide and whose outcome may include stillbirths and enhanced cancer risks and susceptibility to HIV infection. Trichomonas vaginalis relies on imported purine and pyrimidine nucleosides and nucleobases for survival, since it lacks the enzymatic activities necessary for de novo biosynthesis. Here we show that T. vaginalis additionally lacks homologues of the bacterial or mammalian enzymes required for the synthesis of the nicotinamide ring, crucial component in the redox cofactors NAD(+) and NADP. Moreover, we show that a yet fully uncharacterized T. vaginalis protein homologous to bacterial and protozoan nucleoside hydrolases (NHs) is active as a pyrimidine nucleosidase, but shows the highest specificity towards the NAD(+) metabolite nicotinamide riboside. Crystal structures of the trichomonal riboside hydrolase in different states reveals novel intermediates along the NH-catalyzed hydrolytic reaction, including an unexpected asymmetry in the homotetrameric assembly. The active site structure explains the broad specificity towards different ribosides, and offers precise insights for the engineering of specific inhibitors that may simultaneously target different essential pathways in the parasite.

-	~~Authors~~:	+	A riboside hydrolase that salvages both nucleobases and nicotinamide in the auxotrophic parasite Trichomonas vaginalis.,Patrone M, Galasyn GS, Kerin F, Nyitray MM, Parkin DW, Stockman BJ, Degano M J Biol Chem. 2023 Jul 21:105077. doi: 10.1016/j.jbc.2023.105077. PMID:37482279<ref>PMID:37482279</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8oia" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Trichomonas vaginalis]]
		+	[[Category: Degano M]]
		+	[[Category: Patrone M]]
		+	[[Category: Stockman BJ]]

OCA at 18:53, 12 April 2023

OCA — Wed, 12 Apr 2023 18:53:53 GMT

←Older revision		Revision as of 18:53, 12 April 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8oia is ON HOLD	+	The entry 8oia is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8oia" [edit=sysop:move=sysop]

OCA — Wed, 29 Mar 2023 05:09:52 GMT

Protected "8oia" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:09, 29 March 2023

OCA: New page: '''Unreleased structure''' The entry 8oia is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Mar 2023 05:09:51 GMT

New page: '''Unreleased structure''' The entry 8oia is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8oia is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8oia - Revision history

OCA at 10:39, 2 August 2023

OCA at 18:53, 12 April 2023

OCA: Protected "8oia" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8oia is ON HOLD Authors: Description: Category: Unreleased Structures