OCA at 07:03, 18 May 2023

OCA — Thu, 18 May 2023 07:03:16 GMT

←Older revision		Revision as of 07:03, 18 May 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8ojj is ~~ON HOLD~~	+	==Cryo-EM structure of the DnaD-NTD tetramer==
		+	<StructureSection load='8ojj' size='340' side='right'caption='[[8ojj]], [[Resolution\|resolution]] 5.47Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8ojj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OJJ FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ojj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ojj OCA], [https://pdbe.org/8ojj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ojj RCSB], [https://www.ebi.ac.uk/pdbsum/8ojj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ojj ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DNAD_BACSU DNAD_BACSU] Probable component of primosome involved in the initiation of DNA replication.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Genome replication is a fundamental biological activity shared by all organisms. Chromosomal replication proceeds bidirectionally from origins, requiring the loading of two helicases, one for each replisome. However, the molecular mechanisms underpinning helicase loading at bacterial chromosome origins (oriC) are unclear. Here we investigated the essential DNA replication initiation protein DnaD in the model organism Bacillus subtilis. A set of DnaD residues required for ssDNA binding was identified, and photo-crosslinking revealed that this ssDNA binding region interacts preferentially with one strand of oriC. Biochemical and genetic data support the model that DnaD recognizes a new single-stranded DNA (ssDNA) motif located in oriC, the DnaD Recognition Element (DRE). Considered with single particle cryo-electron microscopy (cryo-EM) imaging of DnaD, we propose that the location of the DRE within oriC orchestrates strand-specific recruitment of helicase during DNA replication initiation. These findings significantly advance our mechanistic understanding of bidirectional replication from a bacterial chromosome origin.

-	~~Authors: Winterhalter~~, C., Pelliciari, S., Cronin, N., Costa, ~~T.R.D.~~, Murray, H.~~, Ilangovan, A~~.	+	The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin.,Winterhalter C, Pelliciari S, Stevens D, Fenyk S, Marchand E, Cronin NB, Soultanas P, Costa TRD, Ilangovan A, Murray H Nucleic Acids Res. 2023 Apr 24:gkad277. doi: 10.1093/nar/gkad277. PMID:37093985<ref>PMID:37093985</ref>

-	~~Description: Cryo-EM structure~~ of the ~~DnaD~~-~~NTD tetramer~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Murray, H~~]]	+	<div class="pdbe-citations 8ojj" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Ilangovan, A~~]]	+	== References ==
-	[[Category: ~~Winterhalter, C~~]]	+	<references/>
-	[[Category: ~~Cronin, N~~]]	+	__TOC__
-	[[Category: Pelliciari, S]]	+	</StructureSection>
-	[[Category: ~~Costa, T.R.D~~]]	+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
		+	[[Category: Large Structures]]
		+	[[Category: Costa TRD]]
		+	[[Category: Cronin N]]
		+	[[Category: Ilangovan A]]
		+	[[Category: Murray H]]
		+	[[Category: Pelliciari S]]
		+	[[Category: Winterhalter C]]

OCA at 05:11, 10 May 2023

OCA — Wed, 10 May 2023 05:11:23 GMT

←Older revision		Revision as of 05:11, 10 May 2023
Line 3:		Line 3:
	The entry 8ojj is ON HOLD		The entry 8ojj is ON HOLD

-	Authors: Winterhalter, C., Pelliciari, S., Cronin, N., Costa, T.RD., Murray, H., Ilangovan, A.	+	Authors: Winterhalter, C., Pelliciari, S., Cronin, N., Costa, T.R.D., Murray, H., Ilangovan, A.

	Description: Cryo-EM structure of the DnaD-NTD tetramer		Description: Cryo-EM structure of the DnaD-NTD tetramer
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Costa, T.Rd]]
-	[[Category: Ilangovan, A]]
	[[Category: Murray, H]]		[[Category: Murray, H]]
		+	[[Category: Ilangovan, A]]
	[[Category: Winterhalter, C]]		[[Category: Winterhalter, C]]
	[[Category: Cronin, N]]		[[Category: Cronin, N]]
	[[Category: Pelliciari, S]]		[[Category: Pelliciari, S]]
		+	[[Category: Costa, T.R.D]]

OCA: Protected "8ojj" [edit=sysop:move=sysop]

OCA — Fri, 07 Apr 2023 05:08:44 GMT

Protected "8ojj" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:08, 7 April 2023

OCA: New page: '''Unreleased structure''' The entry 8ojj is ON HOLD Authors: Winterhalter, C., Pelliciari, S., Cronin, N., Costa, T.RD., Murray, H., Ilangovan, A. Description: Cryo-EM structure of th...

OCA — Fri, 07 Apr 2023 05:08:43 GMT

New page: '''Unreleased structure''' The entry 8ojj is ON HOLD Authors: Winterhalter, C., Pelliciari, S., Cronin, N., Costa, T.RD., Murray, H., Ilangovan, A. Description: Cryo-EM structure of th...

New page

'''Unreleased structure'''

The entry 8ojj is ON HOLD

Authors: Winterhalter, C., Pelliciari, S., Cronin, N., Costa, T.RD., Murray, H., Ilangovan, A.

Description: Cryo-EM structure of the DnaD-NTD tetramer
[[Category: Unreleased Structures]]
[[Category: Costa, T.Rd]]
[[Category: Ilangovan, A]]
[[Category: Murray, H]]
[[Category: Winterhalter, C]]
[[Category: Cronin, N]]
[[Category: Pelliciari, S]]

8ojj - Revision history

OCA at 07:03, 18 May 2023

OCA at 05:11, 10 May 2023

OCA: Protected "8ojj" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8ojj is ON HOLD Authors: Winterhalter, C., Pelliciari, S., Cronin, N., Costa, T.RD., Murray, H., Ilangovan, A. Description: Cryo-EM structure of th...