OCA at 08:02, 6 December 2023

OCA — Wed, 06 Dec 2023 08:02:50 GMT

←Older revision		Revision as of 08:02, 6 December 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8olr is ~~ON HOLD until Paper~~ Publication	+	==Structure of yeast 20S proteasome in complex with the natural product beta-lactone inhibitor Cystargolide A==
		+	<StructureSection load='8olr' size='340' side='right'caption='[[8olr]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8olr]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OLR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OLR FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VSZ:Cystargolide+A+(bound)'>VSZ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8olr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8olr OCA], [https://pdbe.org/8olr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8olr RCSB], [https://www.ebi.ac.uk/pdbsum/8olr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8olr ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PSA2_YEAST PSA2_YEAST] The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The caseinolytic protease is a highly conserved serine protease, crucial to prokaryotic and eukaryotic protein homeostasis, and a promising antibacterial and anticancer drug target. Here, we describe the potent cystargolides as the first natural beta-lactone inhibitors of the proteolytic core ClpP. Based on the discovery of two clpP genes next to the cystargolide biosynthetic gene cluster in Kitasatospora cystarginea, we explored ClpP as a potential cystargolide target. We show the inhibition of Staphylococcus aureus ClpP by cystargolide A and B by different biochemical methods in vitro. Synthesis of semi-synthetic derivatives and probes with improved cell penetration allowed us to confirm ClpP as a specific target in S. aureus cells and to demonstrate the anti-virulence activity of this natural product class. Crystal structures show cystargolide A covalently bound to all 14 active sites of ClpP from S. aureus, Aquifex aeolicus, as well as Photorhabdus laumondii, and reveal the molecular mechanism of ClpP inhibition by beta-lactones, the pioneering group of ClpP inhibitors.

-	~~Authors: Illigmann,~~ A., Vielberg~~, M.-T.~~, Lakemeyer, M., Wolf, F~~., Staudt, N.~~, Dema, T., Stange, P., Malik, I., Grond, S., Sieber, ~~S.A.~~, Groll, M., ~~Kaysser, L., Broetz~~-Oesterhelt, H.	+	Structure of Staphylococcus aureus ClpP Bound to the Covalent Active Site Inhibitor Cystargolide A.,Illigmann A, Vielberg MT, Lakemeyer M, Wolf F, Dema T, Stange P, Kuttenlochner W, Liebhart E, Kulik A, Staudt N, Malik I, Grond S, Sieber SA, Kaysser L, Groll M, Brotz-Oesterhelt H Angew Chem Int Ed Engl. 2023 Nov 29:e202314028. doi: 10.1002/anie.202314028. PMID:38029352<ref>PMID:38029352</ref>

-	~~Description: Structure~~ of ~~yeast 20S proteasome in complex with~~ the ~~natural product beta~~-~~lactone inhibitor Cystargolide A~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Wolf, F~~]]	+	<div class="pdbe-citations 8olr" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Vielberg, M.~~-T]]	+	== References ==
-	[[Category: ~~Sieber, S.A~~]]	+	<references/>
-	[[Category: ~~Illigmann, A~~]]	+	__TOC__
-	[[Category: Grond, S]]	+	</StructureSection>
-	[[Category: ~~Stange, P~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Dema, T~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Lakemeyer, M]]	+	[[Category: Broetz-Oesterhelt H]]
-	[[Category: ~~Kaysser, L~~]]	+	[[Category: Dema T]]
-	[[Category: ~~Broetz-Oesterhelt, H~~]]	+	[[Category: Groll M]]
-	[[Category: ~~Groll, M~~]]	+	[[Category: Grond S]]
-	[[Category: ~~Malik, I~~]]	+	[[Category: Illigmann A]]
-	[[Category: ~~Staudt, N~~]]	+	[[Category: Kaysser L]]
		+	[[Category: Lakemeyer M]]
		+	[[Category: Malik I]]
		+	[[Category: Sieber SA]]
		+	[[Category: Stange P]]
		+	[[Category: Staudt N]]
		+	[[Category: Vielberg M-T]]
		+	[[Category: Wolf F]]

OCA at 18:55, 12 April 2023

OCA — Wed, 12 Apr 2023 18:55:05 GMT

←Older revision		Revision as of 18:55, 12 April 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8olr is ON HOLD	+	The entry 8olr is ON HOLD until Paper Publication

	Authors: Illigmann, A., Vielberg, M.-T., Lakemeyer, M., Wolf, F., Staudt, N., Dema, T., Stange, P., Malik, I., Grond, S., Sieber, S.A., Groll, M., Kaysser, L., Broetz-Oesterhelt, H.		Authors: Illigmann, A., Vielberg, M.-T., Lakemeyer, M., Wolf, F., Staudt, N., Dema, T., Stange, P., Malik, I., Grond, S., Sieber, S.A., Groll, M., Kaysser, L., Broetz-Oesterhelt, H.

OCA: Protected "8olr" [edit=sysop:move=sysop]

OCA — Fri, 07 Apr 2023 05:13:32 GMT

Protected "8olr" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:13, 7 April 2023

OCA: New page: '''Unreleased structure''' The entry 8olr is ON HOLD Authors: Illigmann, A., Vielberg, M.-T., Lakemeyer, M., Wolf, F., Staudt, N., Dema, T., Stange, P., Malik, I., Grond, S., Sieber, S....

OCA — Fri, 07 Apr 2023 05:13:29 GMT

New page: '''Unreleased structure''' The entry 8olr is ON HOLD Authors: Illigmann, A., Vielberg, M.-T., Lakemeyer, M., Wolf, F., Staudt, N., Dema, T., Stange, P., Malik, I., Grond, S., Sieber, S....

New page

'''Unreleased structure'''

The entry 8olr is ON HOLD

Authors: Illigmann, A., Vielberg, M.-T., Lakemeyer, M., Wolf, F., Staudt, N., Dema, T., Stange, P., Malik, I., Grond, S., Sieber, S.A., Groll, M., Kaysser, L., Broetz-Oesterhelt, H.

Description: Structure of yeast 20S proteasome in complex with the natural product beta-lactone inhibitor Cystargolide A
[[Category: Unreleased Structures]]
[[Category: Wolf, F]]
[[Category: Vielberg, M.-T]]
[[Category: Sieber, S.A]]
[[Category: Illigmann, A]]
[[Category: Grond, S]]
[[Category: Stange, P]]
[[Category: Dema, T]]
[[Category: Lakemeyer, M]]
[[Category: Kaysser, L]]
[[Category: Broetz-Oesterhelt, H]]
[[Category: Groll, M]]
[[Category: Malik, I]]
[[Category: Staudt, N]]

8olr - Revision history

OCA at 08:02, 6 December 2023

OCA at 18:55, 12 April 2023

OCA: Protected "8olr" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8olr is ON HOLD Authors: Illigmann, A., Vielberg, M.-T., Lakemeyer, M., Wolf, F., Staudt, N., Dema, T., Stange, P., Malik, I., Grond, S., Sieber, S....