OCA at 14:10, 18 October 2023

OCA — Wed, 18 Oct 2023 14:10:12 GMT

←Older revision		Revision as of 14:10, 18 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8oxy is ~~ON HOLD until Paper~~ Publication	+	==Transglutaminase 3 without calcium in complex with DH patient-derived Fab DH63-B02==
		+	<StructureSection load='8oxy' size='340' side='right'caption='[[8oxy]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8oxy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OXY FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8oxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8oxy OCA], [https://pdbe.org/8oxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8oxy RCSB], [https://www.ebi.ac.uk/pdbsum/8oxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8oxy ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TGM3_HUMAN TGM3_HUMAN] Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Transglutaminase 3 (TG3), the autoantigen of dermatitis herpetiformis (DH), is a calcium dependent enzyme that targets glutamine residues in polypeptides for either transamidation or deamidation modifications. To become catalytically active TG3 requires proteolytic cleavage between the core domain and two C-terminal beta-barrels (C1C2). Here, we report four X-ray crystal structures representing inactive and active conformations of human TG3 in complex with a TG3-specific Fab fragment of a DH patient derived antibody. We demonstrate that cleaved TG3, upon binding of a substrate-mimicking inhibitor, undergoes a large conformational change as a beta-sheet in the catalytic core domain moves and C1C2 detaches. The unique enzyme-substrate conformation of TG3 without C1C2 is recognized by DH autoantibodies. The findings support a model where B-cell receptors of TG3-specific B cells bind and internalize TG3-gluten enzyme-substrate complexes thereby facilitating gluten-antigen presentation, T-cell help and autoantibody production.

-	~~Authors~~:	+	Autoantibody binding and unique enzyme-substrate intermediate conformation of human transglutaminase 3.,Heggelund JE, Das S, Stamnaes J, Iversen R, Sollid LM Nat Commun. 2023 Oct 5;14(1):6216. doi: 10.1038/s41467-023-42004-z. PMID:37798283<ref>PMID:37798283</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8oxy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Heggelund JE]]
		+	[[Category: Sollid LM]]

OCA at 05:40, 18 May 2023

OCA — Thu, 18 May 2023 05:40:41 GMT

←Older revision		Revision as of 05:40, 18 May 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8oxy is ON HOLD	+	The entry 8oxy is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8oxy" [edit=sysop:move=sysop]

OCA — Wed, 10 May 2023 05:17:06 GMT

Protected "8oxy" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:17, 10 May 2023

OCA: New page: '''Unreleased structure''' The entry 8oxy is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 May 2023 05:17:05 GMT

New page: '''Unreleased structure''' The entry 8oxy is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8oxy is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8oxy - Revision history

OCA at 14:10, 18 October 2023

OCA at 05:40, 18 May 2023

OCA: Protected "8oxy" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8oxy is ON HOLD Authors: Description: Category: Unreleased Structures