OCA at 08:03, 6 December 2023

OCA — Wed, 06 Dec 2023 08:03:20 GMT

←Older revision		Revision as of 08:03, 6 December 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8p20 is ~~ON HOLD until Paper~~ Publication	+	==TarM(Se)_G117R-UDP-4RboP-glucose==
		+	<StructureSection load='8p20' size='340' side='right'caption='[[8p20]], [[Resolution\|resolution]] 2.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8p20]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8P20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8P20 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.848Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BJT:[(2~{R},3~{S},4~{S})-2-[(2~{S},3~{S},4~{R},5~{R},6~{S})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,4-bis(oxidanyl)-5-[oxidanyl-[(2~{R},3~{S},4~{S})-2,3,4-tris(oxidanyl)-5-[oxidanyl-[(2~{R},3~{S},4~{S})-2,3,4,5-tetrakis(oxidanyl)pentoxy]phosphoryl]oxy-pentoxy]phosphoryl]oxy-pentyl]+[(2~{S},3~{R},4~{R})-2,3,4-tris(oxidanyl)-5-phosphonooxy-pentyl]+hydrogen+phosphate'>BJT</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8p20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8p20 OCA], [https://pdbe.org/8p20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8p20 RCSB], [https://www.ebi.ac.uk/pdbsum/8p20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8p20 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Staphylococcus epidermidis expresses glycerol phosphate wall teichoic acid (WTA), but some health care-associated methicillin-resistant S. epidermidis (HA-MRSE) clones produce a second, ribitol phosphate (RboP) WTA, resembling that of the aggressive pathogen Staphylococcus aureus. RboP-WTA promotes HA-MRSE persistence and virulence in bloodstream infections. We report here that the TarM enzyme of HA-MRSE [TarM(Se)] glycosylates RboP-WTA with glucose, instead of N-acetylglucosamine (GlcNAc) by TarM(Sa) in S. aureus. Replacement of GlcNAc with glucose in RboP-WTA impairs HA-MRSE detection by human immunoglobulin G, which may contribute to the immune-evasion capacities of many invasive S. epidermidis. Crystal structures of complexes with uridine diphosphate glucose (UDP-glucose), and with UDP and glycosylated poly(RboP), reveal the binding mode and glycosylation mechanism of this enzyme and explain why TarM(Se) and TarM(Sa) link different sugars to poly(RboP). These structural data provide evidence that TarM(Se) is a processive WTA glycosyltransferase. Our study will support the targeted inhibition of TarM enzymes, and the development of RboP-WTA targeting vaccines and phage therapies.

-	~~Authors: Guo~~, Y., ~~Stehle~~, T.	+	Invasive Staphylococcus epidermidis uses a unique processive wall teichoic acid glycosyltransferase to evade immune recognition.,Guo Y, Du X, Krusche J, Beck C, Ali S, Walter A, Winstel V, Mayer C, Codee JDC, Peschel A, Stehle T Sci Adv. 2023 Nov 24;9(47):eadj2641. doi: 10.1126/sciadv.adj2641. Epub 2023 Nov , 24. PMID:38000019<ref>PMID:38000019</ref>

-	~~Description: TarM(Se)_G117R-UDP~~-~~4RboP~~-~~glucose~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Stehle, T~~]]	+	<div class="pdbe-citations 8p20" style="background-color:#fffaf0;"></div>
-	[[Category: Guo, Y]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Staphylococcus epidermidis]]
		+	[[Category: Guo Y]]
		+	[[Category: Stehle T]]

OCA at 08:17, 21 June 2023

OCA — Wed, 21 Jun 2023 08:17:11 GMT

←Older revision		Revision as of 08:17, 21 June 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8p20 is ON HOLD	+	The entry 8p20 is ON HOLD until Paper Publication

	Authors: Guo, Y., Stehle, T.		Authors: Guo, Y., Stehle, T.

OCA: Protected "8p20" [edit=sysop:move=sysop]

OCA — Thu, 25 May 2023 02:37:38 GMT

Protected "8p20" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:37, 25 May 2023

OCA: New page: '''Unreleased structure''' The entry 8p20 is ON HOLD Authors: Guo, Y., Stehle, T. Description: TarM(Se)_G117R-UDP-4RboP-glucose Category: Unreleased Structures [[Category: Stehle, ...

OCA — Thu, 25 May 2023 02:37:37 GMT

New page: '''Unreleased structure''' The entry 8p20 is ON HOLD Authors: Guo, Y., Stehle, T. Description: TarM(Se)_G117R-UDP-4RboP-glucose Category: Unreleased Structures [[Category: Stehle, ...

New page

'''Unreleased structure'''

The entry 8p20 is ON HOLD

Authors: Guo, Y., Stehle, T.

Description: TarM(Se)_G117R-UDP-4RboP-glucose
[[Category: Unreleased Structures]]
[[Category: Stehle, T]]
[[Category: Guo, Y]]

8p20 - Revision history

OCA at 08:03, 6 December 2023

OCA at 08:17, 21 June 2023

OCA: Protected "8p20" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8p20 is ON HOLD Authors: Guo, Y., Stehle, T. Description: TarM(Se)_G117R-UDP-4RboP-glucose Category: Unreleased Structures [[Category: Stehle, ...