OCA at 11:45, 13 December 2023

OCA — Wed, 13 Dec 2023 11:45:58 GMT

←Older revision		Revision as of 11:45, 13 December 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8pb9 is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of the c-di-GMP-bound FleQ-FleN master regulator complex from Pseudomonas aeruginosa==
		+	<StructureSection load='8pb9' size='340' side='right'caption='[[8pb9]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8pb9]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PB9 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=C2E:9,9-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d 3,2-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)'>C2E</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pb9 OCA], [https://pdbe.org/8pb9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pb9 RCSB], [https://www.ebi.ac.uk/pdbsum/8pb9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pb9 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FLEQ_PSEAE FLEQ_PSEAE] AAA+ ATPase enhancer-binding protein that acts as a transcription regulator and plays a role in the modulation of mucin adhesion and flagellar gene expression (PubMed:9287015, PubMed:11673434, PubMed:26362077). In addition to flagella genes, regulates also expression of biofilm-related genes (PubMed:22581773). Functions as a transcriptional repressor in the absence of c-di-GMP and as an activator when c-di-GMP is present (PubMed:22581773).<ref>PMID:11673434</ref> <ref>PMID:22581773</ref> <ref>PMID:26362077</ref> <ref>PMID:9287015</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pseudomonas aeruginosa can cause a wide array of chronic and acute infections associated with its ability to rapidly switch between planktonic, biofilm, and dispersed lifestyles, each with a specific arsenal for bacterial survival and virulence. At the cellular level, many of the physiological transitions are orchestrated by the intracellular second messenger c-di-GMP and its receptor-effector FleQ. A bacterial enhancer binding protein, FleQ acts as a master regulator of both flagellar motility and adherence factor secretion and uses remarkably different transcription activation mechanisms depending on its dinucleotide loading state, adenosine triphosphatase (ATPase) activity, interactions with polymerase sigma (sigma) factors, and complexation with a second ATPase, FleN. How the FleQ-FleN tandem can exert diverse effects through recognition of a conserved FleQ binding consensus has remained enigmatic. Here, we provide cryogenic electron microscopy (cryo-EM) structures of both c-di-GMP-bound and c-di-GMP-free FleQ-FleN complexes which deepen our understanding of the proteins' (di)nucleotide-dependent conformational switching and fine-tuned roles in gene expression regulation.

-	~~Authors~~:	+	Structures of the P. aeruginosa FleQ-FleN master regulators reveal large-scale conformational switching in motility and biofilm control.,Torres-Sanchez L, Sana TG, Decossas M, Hashem Y, Krasteva PV Proc Natl Acad Sci U S A. 2023 Dec 12;120(50):e2312276120. doi: , 10.1073/pnas.2312276120. Epub 2023 Dec 5. PMID:38051770<ref>PMID:38051770</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8pb9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseudomonas aeruginosa PAO1]]
		+	[[Category: Krasteva PV]]
		+	[[Category: Torres-Sanchez LT]]

OCA at 06:15, 29 November 2023

OCA — Wed, 29 Nov 2023 06:15:27 GMT

←Older revision		Revision as of 06:15, 29 November 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8pb9 is ON HOLD	+	The entry 8pb9 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 05:37, 22 November 2023

OCA — Wed, 22 Nov 2023 05:37:33 GMT

←Older revision		Revision as of 05:37, 22 November 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8pb9 is ON HOLD ~~until Paper Publication~~	+	The entry 8pb9 is ON HOLD

	Authors:		Authors:

OCA at 05:08, 15 November 2023

OCA — Wed, 15 Nov 2023 05:08:02 GMT

←Older revision		Revision as of 05:08, 15 November 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8pb9 is ON HOLD	+	The entry 8pb9 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8pb9" [edit=sysop:move=sysop]

OCA — Wed, 14 Jun 2023 06:42:37 GMT

Protected "8pb9" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:42, 14 June 2023

OCA: New page: '''Unreleased structure''' The entry 8pb9 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 14 Jun 2023 06:42:34 GMT

New page: '''Unreleased structure''' The entry 8pb9 is ON HOLD Authors: Description: Category: Unreleased Structures

New page