OCA at 13:11, 1 November 2023

OCA — Wed, 01 Nov 2023 13:11:09 GMT

←Older revision		Revision as of 13:11, 1 November 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8pov is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of the C19G/C120G variant of the membrane-bound [NiFe]-Hydrogenase from Cupriavidus necator in the H2-reduced state at 1.92 A Resolution.==
		+	<StructureSection load='8pov' size='340' side='right'caption='[[8pov]], [[Resolution\|resolution]] 1.92Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8pov]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_necator_H16 Cupriavidus necator H16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8POV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8POV FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.92Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NFU:FORMYL[BIS(HYDROCYANATO-1KAPPAC)]IRONNICKEL(FE-NI)'>NFU</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pov OCA], [https://pdbe.org/8pov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pov RCSB], [https://www.ebi.ac.uk/pdbsum/8pov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pov ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MBHL_CUPNH MBHL_CUPNH] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The membrane-bound [NiFe]-hydrogenase of Cupriavidus necator is a rare example of a truly O(2)-tolerant hydrogenase. It catalyzes the oxidation of H(2) into 2e(-) and 2H(+) in the presence of high O(2) concentrations. This characteristic trait is intimately linked to the unique Cys(6)[4Fe-3S] cluster located in the proximal position to the catalytic center and coordinated by six cysteine residues. Two of these cysteines play an essential role in redox-dependent cluster plasticity, which bestows the cofactor with the capacity to mediate two redox transitions at physiological potentials. Here, we investigated the individual roles of the two additional cysteines by replacing them individually as well as simultaneously with glycine. The crystal structures of the corresponding MBH variants revealed the presence of Cys(5)[4Fe-4S] or Cys(4)[4Fe-4S] clusters of different architecture. The protein X-ray crystallography results were correlated with accompanying biochemical, spectroscopic and electrochemical data. The exchanges resulted in a diminished O(2) tolerance of all MBH variants, which was attributed to the fact that the modified proximal clusters mediated only one redox transition. The previously proposed O(2) protection mechanism that detoxifies O(2) to H(2)O using four protons and four electrons supplied by the cofactor infrastructure, is extended by our results, which suggest efficient shutdown of enzyme function by formation of a hydroxy ligand in the active site that protects the enzyme from O(2) binding under electron-deficient conditions.

-	~~Authors: Schmidt~~, A., Kalms, J., ~~Scheerer~~, P.	+	Stepwise conversion of the Cys(6)[4Fe-3S] to a Cys(4)[4Fe-4S] cluster and its impact on the oxygen tolerance of [NiFe]-hydrogenase.,Schmidt A, Kalms J, Lorent C, Katz S, Frielingsdorf S, Evans RM, Fritsch J, Siebert E, Teutloff C, Armstrong FA, Zebger I, Lenz O, Scheerer P Chem Sci. 2023 Sep 20;14(40):11105-11120. doi: 10.1039/d3sc03739h. eCollection , 2023 Oct 18. PMID:37860641<ref>PMID:37860641</ref>

-	~~Description: Crystal Structure of the C19G~~/~~C120G variant~~ of the ~~membrane~~-~~bound [NiFe]~~-~~Hydrogenase from~~ Cupriavidus necator ~~in the H2-reduced state at 1.92 A Resolution.~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Scheerer, P~~]]	+	<div class="pdbe-citations 8pov" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Kalms, J~~]]	+	== References ==
-	[[Category: Schmidt, A]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cupriavidus necator H16]]
		+	[[Category: Large Structures]]
		+	[[Category: Kalms J]]
		+	[[Category: Scheerer P]]
		+	[[Category: Schmidt A]]

OCA at 04:41, 23 August 2023

OCA — Wed, 23 Aug 2023 04:41:10 GMT

←Older revision		Revision as of 04:41, 23 August 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8pov is ON HOLD	+	The entry 8pov is ON HOLD until Paper Publication

	Authors: Schmidt, A., Kalms, J., Scheerer, P.		Authors: Schmidt, A., Kalms, J., Scheerer, P.

OCA: Protected "8pov" [edit=sysop:move=sysop]

OCA — Wed, 12 Jul 2023 04:26:25 GMT

Protected "8pov" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:26, 12 July 2023

OCA: New page: '''Unreleased structure''' The entry 8pov is ON HOLD Authors: Schmidt, A., Kalms, J., Scheerer, P. Description: Crystal Structure of the C19G/C120G variant of the membrane-bound [NiFe]...

OCA — Wed, 12 Jul 2023 04:26:23 GMT

New page: '''Unreleased structure''' The entry 8pov is ON HOLD Authors: Schmidt, A., Kalms, J., Scheerer, P. Description: Crystal Structure of the C19G/C120G variant of the membrane-bound [NiFe]...

New page

'''Unreleased structure'''

The entry 8pov is ON HOLD

Authors: Schmidt, A., Kalms, J., Scheerer, P.

Description: Crystal Structure of the C19G/C120G variant of the membrane-bound [NiFe]-Hydrogenase from Cupriavidus necator in the H2-reduced state at 1.92 A Resolution.
[[Category: Unreleased Structures]]
[[Category: Scheerer, P]]
[[Category: Kalms, J]]
[[Category: Schmidt, A]]

8pov - Revision history

OCA at 13:11, 1 November 2023

OCA at 04:41, 23 August 2023

OCA: Protected "8pov" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8pov is ON HOLD Authors: Schmidt, A., Kalms, J., Scheerer, P. Description: Crystal Structure of the C19G/C120G variant of the membrane-bound [NiFe]...