OCA at 07:32, 22 November 2023

OCA — Wed, 22 Nov 2023 07:32:12 GMT

←Older revision		Revision as of 07:32, 22 November 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8psm is ~~ON HOLD until Paper~~ Publication	+	==Asymmetric unit of the yeast fatty acid synthase in the non-rotated state with ACP at the malonyl/palmitoyl transferase domain (FASx sample)==
		+	<StructureSection load='8psm' size='340' side='right'caption='[[8psm]], [[Resolution\|resolution]] 3.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8psm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PSM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PSM FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8psm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8psm OCA], [https://pdbe.org/8psm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8psm RCSB], [https://www.ebi.ac.uk/pdbsum/8psm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8psm ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FAS2_YEAST FAS2_YEAST] Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The alpha subunit contains domains for: acyl carrier protein, 3-oxoacyl-[acyl-carrier-protein] reductase, and 3-oxoacyl-[acyl-carrier-protein] synthase. This subunit coordinates the binding of the six beta subunits to the enzyme complex.[HAMAP-Rule:MF_00101]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fatty acids (FAs) play a central metabolic role in living cells as constituents of membranes, cellular energy reserves, and second messenger precursors. A 2.6 MDa FA synthase (FAS), where the enzymatic reactions and structures are known, is responsible for FA biosynthesis in yeast. Essential in the yeast FAS catalytic cycle is the acyl carrier protein (ACP) that actively shuttles substrates, biosynthetic intermediates, and products from one active site to another. We resolve the S. cerevisiae FAS structure at 1.9 A, elucidating cofactors and water networks involved in their recognition. Structural snapshots of ACP domains bound to various enzymatic domains allow the reconstruction of a full yeast FA biosynthesis cycle. The structural information suggests that each FAS functional unit could accommodate exogenous proteins to incorporate various enzymatic activities, and we show proof-of-concept experiments where ectopic proteins are used to modulate FAS product profiles.

-	~~Authors: Singh~~, K., Bunzel, G., Graf, B., Yip, K.M., Stark, H., Chari, A.	+	Reconstruction of a fatty acid synthesis cycle from acyl carrier protein and cofactor structural snapshots.,Singh K, Bunzel G, Graf B, Yip KM, Neumann-Schaal M, Stark H, Chari A Cell. 2023 Nov 9;186(23):5054-5067.e16. doi: 10.1016/j.cell.2023.10.009. PMID:37949058<ref>PMID:37949058</ref>

-	~~Description: Asymmetric structure~~ of the ~~yeast fatty acid synthase in the non~~-~~rotated state with ACP at the malonyl~~/~~palmitoyl transferase domain (FASx sample)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Bunzel, G]]	+	<div class="pdbe-citations 8psm" style="background-color:#fffaf0;"></div>
-	[[Category: Chari, A]]	+	== References ==
-	[[Category: ~~Stark, H~~]]	+	<references/>
-	[[Category: Singh, K]]	+	__TOC__
-	[[Category: ~~Graf, B~~]]	+	</StructureSection>
-	[[Category: Yip~~, K.M~~]]	+	[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Bunzel G]]
		+	[[Category: Chari A]]
		+	[[Category: Graf B]]
		+	[[Category: Singh K]]
		+	[[Category: Stark H]]
		+	[[Category: Yip KM]]

OCA at 10:56, 26 July 2023

OCA — Wed, 26 Jul 2023 10:56:57 GMT

←Older revision		Revision as of 10:56, 26 July 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8psm is ON HOLD	+	The entry 8psm is ON HOLD until Paper Publication

	Authors: Singh, K., Bunzel, G., Graf, B., Yip, K.M., Stark, H., Chari, A.		Authors: Singh, K., Bunzel, G., Graf, B., Yip, K.M., Stark, H., Chari, A.
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	Description: Asymmetric structure of the yeast fatty acid synthase in the non-rotated state with ACP at the malonyl/palmitoyl transferase domain (FASx sample)		Description: Asymmetric structure of the yeast fatty acid synthase in the non-rotated state with ACP at the malonyl/palmitoyl transferase domain (FASx sample)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Yip, K.M]]
	[[Category: Bunzel, G]]		[[Category: Bunzel, G]]
	[[Category: Chari, A]]		[[Category: Chari, A]]
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	[[Category: Singh, K]]		[[Category: Singh, K]]
	[[Category: Graf, B]]		[[Category: Graf, B]]
		+	[[Category: Yip, K.M]]

OCA: Protected "8psm" [edit=sysop:move=sysop]

OCA — Wed, 19 Jul 2023 04:57:40 GMT

Protected "8psm" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:57, 19 July 2023

OCA: New page: '''Unreleased structure''' The entry 8psm is ON HOLD Authors: Singh, K., Bunzel, G., Graf, B., Yip, K.M., Stark, H., Chari, A. Description: Asymmetric structure of the yeast fatty acid...

OCA — Wed, 19 Jul 2023 04:57:39 GMT

New page: '''Unreleased structure''' The entry 8psm is ON HOLD Authors: Singh, K., Bunzel, G., Graf, B., Yip, K.M., Stark, H., Chari, A. Description: Asymmetric structure of the yeast fatty acid...

New page

'''Unreleased structure'''

The entry 8psm is ON HOLD

Authors: Singh, K., Bunzel, G., Graf, B., Yip, K.M., Stark, H., Chari, A.

Description: Asymmetric structure of the yeast fatty acid synthase in the non-rotated state with ACP at the malonyl/palmitoyl transferase domain (FASx sample)
[[Category: Unreleased Structures]]
[[Category: Yip, K.M]]
[[Category: Bunzel, G]]
[[Category: Chari, A]]
[[Category: Stark, H]]
[[Category: Singh, K]]
[[Category: Graf, B]]

8psm - Revision history

OCA at 07:32, 22 November 2023

OCA at 10:56, 26 July 2023

OCA: Protected "8psm" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8psm is ON HOLD Authors: Singh, K., Bunzel, G., Graf, B., Yip, K.M., Stark, H., Chari, A. Description: Asymmetric structure of the yeast fatty acid...