OCA at 05:56, 23 August 2023

OCA — Wed, 23 Aug 2023 05:56:07 GMT

←Older revision		Revision as of 05:56, 23 August 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8puh is ~~ON HOLD~~	+	==Structure of the immature HTLV-1 CA lattice from full-length Gag VLPs: CA-CTD refinement==
		+	<StructureSection load='8puh' size='340' side='right'caption='[[8puh]], [[Resolution\|resolution]] 6.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8puh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_T-cell_leukemia_virus_type_I Human T-cell leukemia virus type I]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PUH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PUH FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 6.2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8puh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8puh OCA], [https://pdbe.org/8puh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8puh RCSB], [https://www.ebi.ac.uk/pdbsum/8puh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8puh ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GAG_HTL1A GAG_HTL1A] Matrix protein p19 targets Gag, Gag-Pro and Gag-Pro-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity). Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex (By similarity). Nucleocapsid protein p15 is involved in the packaging and encapsidation of two copies of the genome (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human T-cell leukemia virus type 1 (HTLV-1) has an atypical immature particle morphology compared to other retroviruses. This indicates that these particles are formed in a way that is unique. Here we report the results of cryo-electron tomography (cryo-ET) studies of HTLV-1 virus-like particles (VLPs) assembled in vitro , as well as derived from cells. This work shows that HTLV-1 employs an unconventional mechanism of Gag-Gag interactions to form the immature viral lattice. Analysis of high-resolution structural information from immature CA tubular arrays reveals that the primary stabilizing component in HTLV-1 is CA-NTD. Mutagenesis and biophysical analysis support this observation. This distinguishes HTLV-1 from other retroviruses, in which the stabilization is provided primarily by the CA-CTD. These results are the first to provide structural details of the quaternary arrangement of Gag for an immature deltaretrovirus, and this helps explain why HTLV-1 particles are morphologically distinct.

-	~~Authors:~~ Obr, M., Percipalle, M., Chernikova, D., Yang, H., Thader, A., Pinke, G., Porley, D., Mansky~~, L.M.~~, Dick, R.A.~~, Schur, F~~.K.M.	+	Unconventional stabilization of the human T-cell leukemia virus type 1 immature Gag lattice.,Obr M, Percipalle M, Chernikova D, Yang H, Thader A, Pinke G, Porley D, Mansky LM, Dick RA, Schur FK bioRxiv. 2023 Jul 24:2023.07.24.548988. doi: 10.1101/2023.07.24.548988. Preprint. PMID:37546793<ref>PMID:37546793</ref>

-	~~Description: Structure~~ of the ~~immature HTLV~~-~~1 CA lattice from full~~-~~length Gag VLPs~~: CA-~~CTD refinement~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Chernikova, D]]	+	<div class="pdbe-citations 8puh" style="background-color:#fffaf0;"></div>
-	[[Category: Dick~~, R.A~~]]	+	== References ==
-	[[Category: ~~Thader, A~~]]	+	<references/>
-	[[Category: ~~Yang, H~~]]	+	__TOC__
-	[[Category: ~~Pinke, G~~]]	+	</StructureSection>
-	[[Category: ~~Obr, M~~]]	+	[[Category: Human T-cell leukemia virus type I]]
-	[[Category: ~~Mansky, L.M~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Percipalle, M~~]]	+	[[Category: Chernikova D]]
-	[[Category: ~~Schur, F.K.M~~]]	+	[[Category: Dick RA]]
-	[[Category: ~~Porley, D~~]]	+	[[Category: Mansky LM]]
		+	[[Category: Obr M]]
		+	[[Category: Percipalle M]]
		+	[[Category: Pinke G]]
		+	[[Category: Porley D]]
		+	[[Category: Schur FKM]]
		+	[[Category: Thader A]]
		+	[[Category: Yang H]]

OCA: Protected "8puh" [edit=sysop:move=sysop]

OCA — Wed, 26 Jul 2023 10:59:36 GMT

Protected "8puh" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:59, 26 July 2023

OCA: New page: '''Unreleased structure''' The entry 8puh is ON HOLD Authors: Obr, M., Percipalle, M., Chernikova, D., Yang, H., Thader, A., Pinke, G., Porley, D., Mansky, L.M., Dick, R.A., Schur, F.K....

OCA — Wed, 26 Jul 2023 10:59:35 GMT

New page: '''Unreleased structure''' The entry 8puh is ON HOLD Authors: Obr, M., Percipalle, M., Chernikova, D., Yang, H., Thader, A., Pinke, G., Porley, D., Mansky, L.M., Dick, R.A., Schur, F.K....

New page

'''Unreleased structure'''

The entry 8puh is ON HOLD

Authors: Obr, M., Percipalle, M., Chernikova, D., Yang, H., Thader, A., Pinke, G., Porley, D., Mansky, L.M., Dick, R.A., Schur, F.K.M.

Description: Structure of the immature HTLV-1 CA lattice from full-length Gag VLPs: CA-CTD refinement
[[Category: Unreleased Structures]]
[[Category: Chernikova, D]]
[[Category: Dick, R.A]]
[[Category: Thader, A]]
[[Category: Yang, H]]
[[Category: Pinke, G]]
[[Category: Obr, M]]
[[Category: Mansky, L.M]]
[[Category: Percipalle, M]]
[[Category: Schur, F.K.M]]
[[Category: Porley, D]]

8puh - Revision history

OCA at 05:56, 23 August 2023

OCA: Protected "8puh" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8puh is ON HOLD Authors: Obr, M., Percipalle, M., Chernikova, D., Yang, H., Thader, A., Pinke, G., Porley, D., Mansky, L.M., Dick, R.A., Schur, F.K....