OCA at 07:01, 27 September 2023

OCA — Wed, 27 Sep 2023 07:01:42 GMT

←Older revision		Revision as of 07:01, 27 September 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8q3n is ~~ON HOLD until Paper~~ Publication	+	==Bacterial transcription termination factor Rho + ADP==
		+	<StructureSection load='8q3n' size='340' side='right'caption='[[8q3n]], [[Resolution\|resolution]] 2.63Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8q3n]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8Q3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8Q3N FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.63Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8q3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8q3n OCA], [https://pdbe.org/8q3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8q3n RCSB], [https://www.ebi.ac.uk/pdbsum/8q3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8q3n ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0A0GPI6_ECOLX A0A0A0GPI6_ECOLX] Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.[HAMAP-Rule:MF_01884]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial RNA helicase rho is a genome sentinel that terminates synthesis of damaged and junk RNAs that are not translated by the ribosome. Co-transcriptional RNA surveillance by rho is essential for quality control of the transcriptome during optimal growth. However, it is unclear how bacteria protect their RNAs from overzealous rho during dormancy or stress, conditions common in natural habitats. Here we used cryogenic electron microscopy, biochemical, and genetic approaches to show that residue substitutions, ADP, or ppGpp promote hyper-oligomerization of Escherichia coli rho. Our results demonstrate that nucleotides bound at subunit interfaces control rho switching from active hexamers to inactive higher-order oligomers and extended filaments. Polymers formed upon exposure to antibiotics or ppGpp disassemble when stress is relieved, thereby directly linking termination activity to cellular physiology. Inactivation of rho through hyper-oligomerization is a regulatory strategy shared by RNA polymerases, ribosomes, and metabolic enzymes across all life.

-	~~Authors: Said~~, N., Hilal, T., Wahl, M.C.	+	Transcription termination factor rho polymerizes under stress.,Wang B, Said N, Hilal T, Finazzo M, Wahl MC, Artsimovitch I bioRxiv. 2023 Aug 18:2023.08.18.553922. doi: 10.1101/2023.08.18.553922. Preprint. PMID:37645988<ref>PMID:37645988</ref>

-	~~Description~~: ~~Bacterial transcription termination factor Rho + ADP~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Said, N~~]]	+	<div class="pdbe-citations 8q3n" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Wahl, M.C~~]]	+	== References ==
-	[[Category: ~~Hilal, T~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Hilal T]]
		+	[[Category: Said N]]
		+	[[Category: Wahl MC]]

OCA at 07:25, 30 August 2023

OCA — Wed, 30 Aug 2023 07:25:47 GMT

←Older revision		Revision as of 07:25, 30 August 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8q3n is ON HOLD	+	The entry 8q3n is ON HOLD until Paper Publication

	Authors: Said, N., Hilal, T., Wahl, M.C.		Authors: Said, N., Hilal, T., Wahl, M.C.
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	Description: Bacterial transcription termination factor Rho + ADP		Description: Bacterial transcription termination factor Rho + ADP
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Wahl, M.C]]
	[[Category: Said, N]]		[[Category: Said, N]]
		+	[[Category: Wahl, M.C]]
	[[Category: Hilal, T]]		[[Category: Hilal, T]]

OCA: Protected "8q3n" [edit=sysop:move=sysop]

OCA — Wed, 16 Aug 2023 06:47:34 GMT

Protected "8q3n" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:47, 16 August 2023

OCA: New page: '''Unreleased structure''' The entry 8q3n is ON HOLD Authors: Said, N., Hilal, T., Wahl, M.C. Description: Bacterial transcription termination factor Rho + ADP [[Category: Unreleased S...

OCA — Wed, 16 Aug 2023 06:47:33 GMT

New page: '''Unreleased structure''' The entry 8q3n is ON HOLD Authors: Said, N., Hilal, T., Wahl, M.C. Description: Bacterial transcription termination factor Rho + ADP [[Category: Unreleased S...

New page

'''Unreleased structure'''

The entry 8q3n is ON HOLD

Authors: Said, N., Hilal, T., Wahl, M.C.

Description: Bacterial transcription termination factor Rho + ADP
[[Category: Unreleased Structures]]
[[Category: Wahl, M.C]]
[[Category: Said, N]]
[[Category: Hilal, T]]

8q3n - Revision history

OCA at 07:01, 27 September 2023

OCA at 07:25, 30 August 2023

OCA: Protected "8q3n" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8q3n is ON HOLD Authors: Said, N., Hilal, T., Wahl, M.C. Description: Bacterial transcription termination factor Rho + ADP [[Category: Unreleased S...