OCA at 05:21, 15 May 2024

OCA — Wed, 15 May 2024 05:21:20 GMT

←Older revision		Revision as of 05:21, 15 May 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8q5v is ~~ON HOLD until Paper~~ Publication	+	==MgADP-bound Fe protein of the molybdenum nitrogenase from Methanothermococcus thermolithotrophicus==
		+	<StructureSection load='8q5v' size='340' side='right'caption='[[8q5v]], [[Resolution\|resolution]] 2.74Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8q5v]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus_DSM_2095 Methanothermococcus thermolithotrophicus DSM 2095]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8Q5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8Q5V FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.74Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8q5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8q5v OCA], [https://pdbe.org/8q5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8q5v RCSB], [https://www.ebi.ac.uk/pdbsum/8q5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8q5v ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NIFH1_METTL NIFH1_METTL] The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The nitrogenase reductase NifH catalyses ATP-dependent electron delivery to the Mo-nitrogenase, a reaction central to biological dinitrogen (N(2)) fixation. While NifHs have been extensively studied in bacteria, structural information about their archaeal counterparts is limited. Archaeal NifHs are considered more ancient, particularly those from Methanococcales, a group of marine hydrogenotrophic methanogens, which includes diazotrophs growing at temperatures near 92 degrees C. Here, we structurally and biochemically analyse NifHs from three Methanococcales, offering the X-ray crystal structures from meso-, thermo-, and hyperthermophilic methanogens. While NifH from Methanococcus maripaludis (37 degrees C) was obtained through heterologous recombinant expression, the proteins from Methanothermococcus thermolithotrophicus (65 degrees C) and Methanocaldococcus infernus (85 degrees C) were natively purified from the diazotrophic archaea. The structures from M. thermolithotrophicus crystallised as isolated exhibit high flexibility. In contrast, the complexes of NifH with MgADP obtained from the three methanogens are superposable, more rigid, and present remarkable structural conservation with their homologues. They retain key structural features of P-loop NTPases and share similar electrostatic profiles with the counterpart from the bacterial model organism Azotobacter vinelandii. In comparison to the NifH from the phylogenetically distant Methanosarcina acetivorans, these reductases do not cross-react significantly with Mo-nitrogenase from A. vinelandii. However, they associate with bacterial nitrogenase when ADP. AlF4- is added to mimic a transient reactive state. Accordingly, detailed surface analyses suggest that subtle substitutions would affect optimal binding during the catalytic cycle between the NifH from Methanococcales and the bacterial nitrogenase, implying differences in the N(2)-machinery from these ancient archaea.

-	~~Authors: Maslac~~, N., ~~Wagner~~, T.	+	Structural comparison of (hyper-)thermophilic nitrogenase reductases from three marine Methanococcales.,Maslac N, Cadoux C, Bolte P, Murken F, Gu W, Milton RD, Wagner T FEBS J. 2024 May 2. doi: 10.1111/febs.17148. PMID:38696373<ref>PMID:38696373</ref>

-	~~Description: MgADP-bound Fe protein~~ of the ~~molybdenum nitrogenase from Methanothermococcus thermolithotrophicus~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Wagner, T~~]]	+	<div class="pdbe-citations 8q5v" style="background-color:#fffaf0;"></div>
-	[[Category: Maslac, N]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Methanothermococcus thermolithotrophicus DSM 2095]]
		+	[[Category: Maslac N]]
		+	[[Category: Wagner T]]

OCA at 07:26, 30 August 2023

OCA — Wed, 30 Aug 2023 07:26:00 GMT

←Older revision		Revision as of 07:26, 30 August 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8q5v is ON HOLD	+	The entry 8q5v is ON HOLD until Paper Publication

	Authors: Maslac, N., Wagner, T.		Authors: Maslac, N., Wagner, T.

OCA: Protected "8q5v" [edit=sysop:move=sysop]

OCA — Wed, 16 Aug 2023 06:51:54 GMT

Protected "8q5v" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:51, 16 August 2023

OCA: New page: '''Unreleased structure''' The entry 8q5v is ON HOLD Authors: Maslac, N., Wagner, T. Description: MgADP-bound Fe protein of the molybdenum nitrogenase from Methanothermococcus thermoli...

OCA — Wed, 16 Aug 2023 06:51:53 GMT

New page: '''Unreleased structure''' The entry 8q5v is ON HOLD Authors: Maslac, N., Wagner, T. Description: MgADP-bound Fe protein of the molybdenum nitrogenase from Methanothermococcus thermoli...

New page

'''Unreleased structure'''

The entry 8q5v is ON HOLD

Authors: Maslac, N., Wagner, T.

Description: MgADP-bound Fe protein of the molybdenum nitrogenase from Methanothermococcus thermolithotrophicus
[[Category: Unreleased Structures]]
[[Category: Wagner, T]]
[[Category: Maslac, N]]

8q5v - Revision history

OCA at 05:21, 15 May 2024

OCA at 07:26, 30 August 2023

OCA: Protected "8q5v" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8q5v is ON HOLD Authors: Maslac, N., Wagner, T. Description: MgADP-bound Fe protein of the molybdenum nitrogenase from Methanothermococcus thermoli...