OCA at 05:21, 15 May 2024

OCA — Wed, 15 May 2024 05:21:22 GMT

←Older revision		Revision as of 05:21, 15 May 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8q8h is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of Apo beta-D-GalNAcase from Niabella aurantiaca (Structure 2)==
		+	<StructureSection load='8q8h' size='340' side='right'caption='[[8q8h]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8q8h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Niabella_aurantiaca_DSM_17617 Niabella aurantiaca DSM 17617]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8Q8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8Q8H FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8q8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8q8h OCA], [https://pdbe.org/8q8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8q8h RCSB], [https://www.ebi.ac.uk/pdbsum/8q8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8q8h ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We report here the identification, characterization and three-dimensional (3D) structure determination of NaNga, a newly identified beta-N-acetylgalactosaminidase from the Gram-negative soil bacterium Niabella aurantiaca DSM 17617. When recombinantly expressed in Escherichia coli, the enzyme selectively cleaved 4-nitrophenyl-N-acetyl-beta-d-galactosamine (pNP-beta-d-GalpNAc). The X-ray crystal structure of the protein was refined to 2.5 A and consists of an N-terminal beta-sandwich domain and a (beta/alpha)(8) barrel catalytic domain. Despite a mere 22% sequence identity, the 3D structure of NaNga is similar to those previously determined for family GH123 members, suggesting it also employs the same substrate-assisted catalytic mechanism. Inhibition by N-acetyl-galactosamine thiazoline (GalNAc-thiazoline) supports the suggested mechanism. A phylogenetic analysis of its proximal sequence space shows significant clustering of unknown sequences around NaNga with sufficient divergence with previously identified GH123 members to subdivide this family into distinct subfamilies. Although the actual biological substrate of our enzyme remains unknown, examination of the active site pocket suggests that it may be a beta-N-acetylgalactosaminide substituted by a monosaccharide at O-3. Analysis of the genomic context suggests, in turn, that this substituted beta-N-acetylgalactosaminide may be appended to a d-arabinan from an environmental Actinomycete.

-	~~Authors: Morth, J.P~~., Moreno Prieto, E.S., Siebenhaar, S.	+	Characterization and structural study of a novel beta-N-acetylgalactosaminidase from Niabella aurantiaca.,Moreno Prieto ES, Fjermedal S, Siebenhaar S, Vuillemin M, Holck J, Vincentelli R, Gippert GP, Wilkens C, Morth JP, Henrissat B FEBS J. 2024 Apr;291(7):1439-1456. doi: 10.1111/febs.17042. Epub 2024 Jan 4. PMID:38129294<ref>PMID:38129294</ref>

-	~~Description: Crystal Structure~~ of ~~Apo beta~~-D-~~GalNAcase from Niabella aurantiaca (Structure 2)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Siebenhaar, S~~]]	+	<div class="pdbe-citations 8q8h" style="background-color:#fffaf0;"></div>
-	[[Category: Moreno Prieto~~, E.S~~]]	+	== References ==
-	[[Category: Morth~~, J.P~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Niabella aurantiaca DSM 17617]]
		+	[[Category: Moreno Prieto ES]]
		+	[[Category: Morth JP]]
		+	[[Category: Siebenhaar S]]

OCA at 04:36, 11 October 2023

OCA — Wed, 11 Oct 2023 04:36:03 GMT

←Older revision		Revision as of 04:36, 11 October 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8q8h is ON HOLD	+	The entry 8q8h is ON HOLD until Paper Publication

	Authors: Morth, J.P., Moreno Prieto, E.S., Siebenhaar, S.		Authors: Morth, J.P., Moreno Prieto, E.S., Siebenhaar, S.

OCA at 12:04, 20 September 2023

OCA — Wed, 20 Sep 2023 12:04:57 GMT

←Older revision		Revision as of 12:04, 20 September 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8q8h is ON HOLD ~~until sometime in the future~~	+	The entry 8q8h is ON HOLD

	Authors: Morth, J.P., Moreno Prieto, E.S., Siebenhaar, S.		Authors: Morth, J.P., Moreno Prieto, E.S., Siebenhaar, S.

OCA at 04:28, 6 September 2023

OCA — Wed, 06 Sep 2023 04:28:25 GMT

←Older revision		Revision as of 04:28, 6 September 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8q8h is ON HOLD	+	The entry 8q8h is ON HOLD until sometime in the future

	Authors: Morth, J.P., Moreno Prieto, E.S., Siebenhaar, S.		Authors: Morth, J.P., Moreno Prieto, E.S., Siebenhaar, S.

OCA: Protected "8q8h" [edit=sysop:move=sysop]

OCA — Wed, 30 Aug 2023 07:29:50 GMT

Protected "8q8h" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:29, 30 August 2023

OCA: New page: '''Unreleased structure''' The entry 8q8h is ON HOLD Authors: Morth, J.P., Moreno Prieto, E.S., Siebenhaar, S. Description: Crystal Structure of Apo beta-D-GalNAcase from Niabella aura...

OCA — Wed, 30 Aug 2023 07:29:47 GMT

New page: '''Unreleased structure''' The entry 8q8h is ON HOLD Authors: Morth, J.P., Moreno Prieto, E.S., Siebenhaar, S. Description: Crystal Structure of Apo beta-D-GalNAcase from Niabella aura...

New page