OCA at 23:14, 27 December 2023

OCA — Wed, 27 Dec 2023 23:14:55 GMT

←Older revision		Revision as of 23:14, 27 December 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8qk8 is ~~ON HOLD until Paper~~ Publication	+	==Structure of Legionella pneumophila Lcl C-terminal domain bound to sulphate==
		+	<StructureSection load='8qk8' size='340' side='right'caption='[[8qk8]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8qk8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_130b Legionella pneumophila 130b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QK8 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qk8 OCA], [https://pdbe.org/8qk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qk8 RCSB], [https://www.ebi.ac.uk/pdbsum/8qk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qk8 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/E7BLH6_LEGPN E7BLH6_LEGPN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial adhesion is a fundamental process which enables colonisation of niche environments and is key for infection. However, in Legionella pneumophila, the causative agent of Legionnaires' disease, these processes are not well understood. The Legionella collagen-like protein (Lcl) is an extracellular peripheral membrane protein that recognises sulphated glycosaminoglycans (GAGs) on the surface of eukaryotic cells, but also stimulates bacterial aggregation in response to divalent cations. Here we report the crystal structure of the Lcl C-terminal domain (Lcl-CTD) and present a model for intact Lcl. Our data reveal that Lcl-CTD forms an unusual dynamic trimer arrangement with a positively charged external surface and a negatively charged solvent exposed internal cavity. Through Molecular Dynamics (MD) simulations, we show how the GAG chondroitin-4-sulphate associates with the Lcl-CTD surface via unique binding modes. Our findings show that Lcl homologs are present across both the Pseudomonadota and Fibrobacterota-Chlorobiota-Bacteroidota phyla and suggest that Lcl may represent a versatile carbohydrate binding mechanism.

-	~~Authors~~:	+	The Legionella collagen-like protein employs a unique binding mechanism for the recognition of host glycosaminoglycans.,Rehman S, Antonovic AK, McIntire IE, Zheng H, Cleaver L, Adams CO, Portlock T, Richardson K, Shaw R, Oregioni A, Mastroianni G, Whittaker SB, Kelly G, Fornili A, Cianciotto NP, Garnett JA bioRxiv. 2023 Dec 10:2023.12.10.570962. doi: 10.1101/2023.12.10.570962. Preprint. PMID:38106198<ref>PMID:38106198</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8qk8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Legionella pneumophila 130b]]
		+	[[Category: Garnett JA]]
		+	[[Category: Rehman S]]

OCA at 12:07, 18 October 2023

OCA — Wed, 18 Oct 2023 12:07:02 GMT

←Older revision		Revision as of 12:07, 18 October 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8qk8 is ON HOLD	+	The entry 8qk8 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8qk8" [edit=sysop:move=sysop]

OCA — Wed, 20 Sep 2023 12:22:47 GMT

Protected "8qk8" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:22, 20 September 2023

OCA: New page: '''Unreleased structure''' The entry 8qk8 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Sep 2023 12:22:46 GMT

New page: '''Unreleased structure''' The entry 8qk8 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8qk8 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8qk8 - Revision history

OCA at 23:14, 27 December 2023

OCA at 12:07, 18 October 2023

OCA: Protected "8qk8" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8qk8 is ON HOLD Authors: Description: Category: Unreleased Structures