OCA at 07:32, 22 November 2023

OCA — Wed, 22 Nov 2023 07:32:34 GMT

←Older revision		Revision as of 07:32, 22 November 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8qmk is ~~ON HOLD until Paper~~ Publication	+	==Enzymatically-produced complex-B bound TmHydE structure==
		+	<StructureSection load='8qmk' size='340' side='right'caption='[[8qmk]], [[Resolution\|resolution]] 1.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8qmk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QMK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QMK FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=CPS:3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE'>CPS</scene>, <scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qmk OCA], [https://pdbe.org/8qmk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qmk RCSB], [https://www.ebi.ac.uk/pdbsum/8qmk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qmk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/HYDE_THEMA HYDE_THEMA] Required for the maturation of the [FeFe]-hydrogenase HydA (By similarity). Catalyzes the reductive cleavage of S-adenosyl-L-methionine (in vitro), suggesting it may contribute to the biosynthesis of an essential sulfur-containing ligand that binds to the hydrogenase active site [2Fe-2S] cluster (PubMed:16137685).[UniProtKB:Q97IK9]<ref>PMID:16137685</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	FeFe]-hydrogenases efficiently catalyze the reversible oxidation of molecular hydrogen. Their prowess stems from the intricate H-cluster, combining a [Fe4S4] center with a binuclear iron center ([2Fe]H). In the latter, each iron atom is coordinated by a CO and CN ligand, connected by a CO and an azadithiolate ligand. The synthesis of this active site involves a unique multiprotein assembly, featuring radical SAM proteins HydG and HydE. HydG initiates the transformation of L-tyrosine into cyanide and carbon monoxide to generate complex-B, which is subsequently transferred to HydE to continue the biosynthesis of the [2Fe]H-subcluster. Due to its instability, complex-B isolation for structural or spectroscopic characterization has been elusive this far. Nevertheless, the use of a biomimetic compound of complex-B allowed circumventing the need for the HydG protein during in vitro functional investigations, implying a similar structure for complex-B. Here, we used the HydE protein as a nanocage to encapsulate and stabilize the complex-B product generated by HydG. Using X-ray crystallography, we successfully determined its structure at 1.3 A resolution. Furthermore, we demonstrated that complex-B is directly transferred from HydG to HydE, thus not being released into the solution post-synthesis, highlighting a transient interaction between the two proteins.

-	~~Authors~~: ~~Omeiri~~, J., Martin, L., Usclat, A., Cherrier~~, M.V.~~, Nicolet, Y.	+	Maturation of the [FeFe]-Hydrogenase: Direct Transfer of the (kappa3-cysteinate)FeII(CN)(CO)2 Complex-B from HydG to HydE.,Omeiri J, Martin L, Usclat A, Cherrier MV, Nicolet Y Angew Chem Int Ed Engl. 2023 Nov 14:e202314819. doi: 10.1002/anie.202314819. PMID:37962296<ref>PMID:37962296</ref>

-	~~Description: Enzymatically~~-~~produced complex~~-~~B bound TmHydE structure~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Martin, L~~]]	+	<div class="pdbe-citations 8qmk" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Omeiri, J~~]]	+	== References ==
-	[[Category: Nicolet, Y]]	+	<references/>
-	[[Category: ~~Usclat, A~~]]	+	__TOC__
-	[[Category: ~~Cherrier, M.V~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Thermotoga maritima MSB8]]
		+	[[Category: Cherrier MV]]
		+	[[Category: Martin L]]
		+	[[Category: Nicolet Y]]
		+	[[Category: Omeiri J]]
		+	[[Category: Usclat A]]

OCA: Protected "8qmk" [edit=sysop:move=sysop]

OCA — Wed, 04 Oct 2023 10:50:30 GMT

Protected "8qmk" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:50, 4 October 2023

OCA: New page: '''Unreleased structure''' The entry 8qmk is ON HOLD until Paper Publication Authors: Omeiri, J., Martin, L., Usclat, A., Cherrier, M.V., Nicolet, Y. Description: Enzymatically-produce...

OCA — Wed, 04 Oct 2023 10:50:29 GMT

New page: '''Unreleased structure''' The entry 8qmk is ON HOLD until Paper Publication Authors: Omeiri, J., Martin, L., Usclat, A., Cherrier, M.V., Nicolet, Y. Description: Enzymatically-produce...

New page

'''Unreleased structure'''

The entry 8qmk is ON HOLD until Paper Publication

Authors: Omeiri, J., Martin, L., Usclat, A., Cherrier, M.V., Nicolet, Y.

Description: Enzymatically-produced complex-B bound TmHydE structure
[[Category: Unreleased Structures]]
[[Category: Martin, L]]
[[Category: Omeiri, J]]
[[Category: Nicolet, Y]]
[[Category: Usclat, A]]
[[Category: Cherrier, M.V]]

8qmk - Revision history

OCA at 07:32, 22 November 2023

OCA: Protected "8qmk" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8qmk is ON HOLD until Paper Publication Authors: Omeiri, J., Martin, L., Usclat, A., Cherrier, M.V., Nicolet, Y. Description: Enzymatically-produce...