8qq8 - Revision history

OCA at 18:04, 29 May 2024

OCA — Wed, 29 May 2024 18:04:11 GMT

←Older revision		Revision as of 18:04, 29 May 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8qq8 is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of F420-dependent Methylene-Tetrahydromethanopterin Reductase Mutant E6Q from Methanocaldococcus Jannaschii==
		+	<StructureSection load='8qq8' size='340' side='right'caption='[[8qq8]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8qq8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QQ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QQ8 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qq8 OCA], [https://pdbe.org/8qq8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qq8 RCSB], [https://www.ebi.ac.uk/pdbsum/8qq8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qq8 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A832SYB5_9EURY A0A832SYB5_9EURY] Catalyzes the reversible reduction of methylene-H(4)MPT to methyl-H(4)MPT.[HAMAP-Rule:MF_01091]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Methylene-tetrahydropterin reductases catalyze the reduction of a methylene to a methyl group bound to a reduced pterin as C(1) carrier in various one-carbon (C(1)) metabolisms. F(420)-dependent methylene-tetrahydromethanopterin (methylene-H(4)MPT) reductase (Mer) and the flavin-independent methylene-tetrahydrofolate (methylene-H(4)F) reductase (Mfr) use a ternary complex mechanism for the direct transfer of a hydride from F(420)H(2) and NAD(P)H to the respective methylene group, whereas FAD-dependent methylene-H(4)F reductase (MTHFR) uses FAD as prosthetic group and a ping-pong mechanism to catalyze the reduction of methylene-H(4)F. A ternary complex structure and a thereof derived catalytic mechanism of MTHFR is available, while no ternary complex structures of Mfr or Mer are reported. Here, Mer from Methanocaldococcus jannaschii (jMer) was heterologously produced and the crystal structures of the enzyme with and without F(420) were determined. A ternary complex of jMer was modeled on the basis of the jMer-F(420) structure and the ternary complex structure of MTHFR by superimposing the polypeptide after fixing hydride-transferring atoms of the flavins on each other, and by the subsequent transfer of the methyl-tetrahydropterin from MTHFR to jMer. Mutational analysis of four functional amino acids, which are similarly positioned in the three reductase structures, indicated despite the insignificant sequence identity, a common catalytic mechanism with a 5-iminium cation of methylene-tetrahydropterin as intermediate protonated by a shared glutamate. According to structural, mutational and phylogenetic analysis, the evolution of the three reductases most likely proceeds via a convergent development although a divergent scenario requiring drastic structural changes of the common ancestor cannot be completely ruled out.

-	~~Authors: Gehl~~, M., Demmer, U., Ermler, U., Shima, S.	+	Mutational and structural studies of (betaalpha)(8)-barrel fold methylene-tetrahydropterin reductases utilizing a common catalytic mechanism.,Gehl M, Demmer U, Ermler U, Shima S Protein Sci. 2024 Jun;33(6):e5018. doi: 10.1002/pro.5018. PMID:38747406<ref>PMID:38747406</ref>

-	~~Description: Crystal Structure~~ of ~~F420~~-~~dependent Methylene~~-~~Tetrahydromethanopterin Reductase Mutant E6Q from Methanocaldococcus Jannaschii~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Gehl, M~~]]	+	<div class="pdbe-citations 8qq8" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Shima, S~~]]	+	== References ==
-	[[Category: Ermler, U]]	+	<references/>
-	[[Category: ~~Demmer, U~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Methanocaldococcus jannaschii]]
		+	[[Category: Demmer U]]
		+	[[Category: Ermler U]]
		+	[[Category: Gehl M]]
		+	[[Category: Shima S]]

OCA at 06:20, 29 November 2023

OCA — Wed, 29 Nov 2023 06:20:53 GMT

←Older revision		Revision as of 06:20, 29 November 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8qq8 is ON HOLD	+	The entry 8qq8 is ON HOLD until Paper Publication

	Authors: Gehl, M., Demmer, U., Ermler, U., Shima, S.		Authors: Gehl, M., Demmer, U., Ermler, U., Shima, S.

OCA at 05:49, 22 November 2023

OCA — Wed, 22 Nov 2023 05:49:21 GMT

←Older revision		Revision as of 05:49, 22 November 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8qq8 is ON HOLD ~~until Paper Publication~~	+	The entry 8qq8 is ON HOLD

	Authors: Gehl, M., Demmer, U., Ermler, U., Shima, S.		Authors: Gehl, M., Demmer, U., Ermler, U., Shima, S.

OCA at 05:16, 15 November 2023

OCA — Wed, 15 Nov 2023 05:16:38 GMT

←Older revision		Revision as of 05:16, 15 November 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8qq8 is ON HOLD	+	The entry 8qq8 is ON HOLD until Paper Publication

	Authors: Gehl, M., Demmer, U., Ermler, U., Shima, S.		Authors: Gehl, M., Demmer, U., Ermler, U., Shima, S.
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	Description: Crystal Structure of F420-dependent Methylene-Tetrahydromethanopterin Reductase Mutant E6Q from Methanocaldococcus Jannaschii		Description: Crystal Structure of F420-dependent Methylene-Tetrahydromethanopterin Reductase Mutant E6Q from Methanocaldococcus Jannaschii
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Shima, S]]
-	[[Category: Demmer, U]]
	[[Category: Gehl, M]]		[[Category: Gehl, M]]
		+	[[Category: Shima, S]]
	[[Category: Ermler, U]]		[[Category: Ermler, U]]
		+	[[Category: Demmer, U]]

OCA: Protected "8qq8" [edit=sysop:move=sysop]

OCA — Wed, 11 Oct 2023 04:42:42 GMT

Protected "8qq8" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:42, 11 October 2023

OCA: New page: '''Unreleased structure''' The entry 8qq8 is ON HOLD Authors: Gehl, M., Demmer, U., Ermler, U., Shima, S. Description: Crystal Structure of F420-dependent Methylene-Tetrahydromethanopt...

OCA — Wed, 11 Oct 2023 04:42:41 GMT

New page: '''Unreleased structure''' The entry 8qq8 is ON HOLD Authors: Gehl, M., Demmer, U., Ermler, U., Shima, S. Description: Crystal Structure of F420-dependent Methylene-Tetrahydromethanopt...

New page

'''Unreleased structure'''

The entry 8qq8 is ON HOLD

Authors: Gehl, M., Demmer, U., Ermler, U., Shima, S.

Description: Crystal Structure of F420-dependent Methylene-Tetrahydromethanopterin Reductase Mutant E6Q from Methanocaldococcus Jannaschii
[[Category: Unreleased Structures]]
[[Category: Shima, S]]
[[Category: Demmer, U]]
[[Category: Gehl, M]]
[[Category: Ermler, U]]