OCA at 23:15, 27 December 2023

OCA — Wed, 27 Dec 2023 23:15:07 GMT

←Older revision		Revision as of 23:15, 27 December 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8qz8 is ~~ON HOLD until Paper~~ Publication	+	==Tilapia Lake Virus polymerase in vRNA pre-termination state (transcriptase conformation)==
		+	<StructureSection load='8qz8' size='340' side='right'caption='[[8qz8]], [[Resolution\|resolution]] 3.13Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8qz8]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Tilapia_lake_virus Tilapia lake virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QZ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QZ8 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.13Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qz8 OCA], [https://pdbe.org/8qz8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qz8 RCSB], [https://www.ebi.ac.uk/pdbsum/8qz8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qz8 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A142I7Z3_9VIRU A0A142I7Z3_9VIRU]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tilapia Lake Virus (TiLV), a recently discovered pathogen of tilapia fish, belongs to the Amnoonviridae family from the Articulavirales order. Its ten genome segments have characteristic conserved ends and encode proteins with no known homologues, apart from the segment 1, which encodes an orthomyxo-like RNA-dependent-RNA polymerase core subunit. Here we show that segments 1-3 encode respectively the PB1, PB2 and PA-like subunits of an active heterotrimeric polymerase that maintains all domains found in the distantly related influenza polymerase, despite an unprecedented overall size reduction of 40%. Multiple high-resolution cryo-EM structures of TiLV polymerase in pre-initiation, initiation and active elongation states, show how it binds the vRNA and cRNA promoters and performs RNA synthesis, with both transcriptase and replicase configurations being characterised. However, the highly truncated endonuclease-like domain appears inactive and the putative cap-binding domain is autoinhibited, emphasising that many functional aspects of TiLV polymerase remain to be elucidated.

-	~~Authors: Arragain~~, B., ~~Cusack~~, S.	+	Structural and functional analysis of the minimal orthomyxovirus-like polymerase of Tilapia Lake Virus from the highly diverged Amnoonviridae family.,Arragain B, Pelosse M, Thompson A, Cusack S Nat Commun. 2023 Dec 9;14(1):8145. doi: 10.1038/s41467-023-44044-x. PMID:38066000<ref>PMID:38066000</ref>

-	~~Description: Tilapia Lake Virus polymerase in vRNA pre~~-~~termination state (transcriptase conformation)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Arragain, B]]	+	<div class="pdbe-citations 8qz8" style="background-color:#fffaf0;"></div>
-	[[Category: Cusack, S]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Tilapia lake virus]]
		+	[[Category: Arragain B]]
		+	[[Category: Cusack S]]

OCA at 06:38, 29 November 2023

OCA — Wed, 29 Nov 2023 06:38:12 GMT

←Older revision		Revision as of 06:38, 29 November 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8qz8 is ON HOLD	+	The entry 8qz8 is ON HOLD until Paper Publication

	Authors: Arragain, B., Cusack, S.		Authors: Arragain, B., Cusack, S.

OCA: Protected "8qz8" [edit=sysop:move=sysop]

OCA — Wed, 01 Nov 2023 10:47:12 GMT

Protected "8qz8" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:47, 1 November 2023

OCA: New page: '''Unreleased structure''' The entry 8qz8 is ON HOLD Authors: Arragain, B., Cusack, S. Description: Tilapia Lake Virus polymerase in vRNA pre-termination state (transcriptase conformat...

OCA — Wed, 01 Nov 2023 10:47:11 GMT

New page: '''Unreleased structure''' The entry 8qz8 is ON HOLD Authors: Arragain, B., Cusack, S. Description: Tilapia Lake Virus polymerase in vRNA pre-termination state (transcriptase conformat...

New page

'''Unreleased structure'''

The entry 8qz8 is ON HOLD

Authors: Arragain, B., Cusack, S.

Description: Tilapia Lake Virus polymerase in vRNA pre-termination state (transcriptase conformation)
[[Category: Unreleased Structures]]
[[Category: Arragain, B]]
[[Category: Cusack, S]]

8qz8 - Revision history

OCA at 23:15, 27 December 2023

OCA at 06:38, 29 November 2023

OCA: Protected "8qz8" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8qz8 is ON HOLD Authors: Arragain, B., Cusack, S. Description: Tilapia Lake Virus polymerase in vRNA pre-termination state (transcriptase conformat...