OCA at 08:31, 16 August 2023

OCA — Wed, 16 Aug 2023 08:31:18 GMT

←Older revision		Revision as of 08:31, 16 August 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8sk5 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the SARS-CoV-2 neutralizing VHH 7A9 bound to the spike receptor binding domain==
-		+	<StructureSection load='8sk5' size='340' side='right'caption='[[8sk5]], [[Resolution\|resolution]] 2.01Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[8sk5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SK5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SK5 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.011Å</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8sk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8sk5 OCA], [https://pdbe.org/8sk5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8sk5 RCSB], [https://www.ebi.ac.uk/pdbsum/8sk5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8sk5 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Lama glama]]
		+	[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Eddins M]]
		+	[[Category: Galli J]]
		+	[[Category: Gomez-Llorente Y]]
		+	[[Category: Noland CL]]
		+	[[Category: Pande K]]
		+	[[Category: Zhang L]]
		+	[[Category: Zhou H]]

OCA: Protected "8sk5" [edit=sysop:move=sysop]

OCA — Wed, 26 Apr 2023 15:28:29 GMT

Protected "8sk5" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:28, 26 April 2023

OCA: New page: '''Unreleased structure''' The entry 8sk5 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Apr 2023 15:28:27 GMT

New page: '''Unreleased structure''' The entry 8sk5 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8sk5 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

8sk5 - Revision history

OCA at 08:31, 16 August 2023

OCA: Protected "8sk5" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8sk5 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures