OCA at 08:35, 9 May 2024

OCA — Thu, 09 May 2024 08:35:22 GMT

←Older revision		Revision as of 08:35, 9 May 2024
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8ssl is ~~ON HOLD until Paper~~ Publication	+	==Isobutyryl-CoA mutase fused Q341A in the presence of GTP==
		+	<StructureSection load='8ssl' size='340' side='right'caption='[[8ssl]], [[Resolution\|resolution]] 4.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8ssl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans_CH34 Cupriavidus metallidurans CH34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SSL FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ssl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ssl OCA], [https://pdbe.org/8ssl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ssl RCSB], [https://www.ebi.ac.uk/pdbsum/8ssl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ssl ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ICMF_CUPMC ICMF_CUPMC] Catalyzes the reversible interconversion of isobutyryl-CoA and n-butyryl-CoA, and to a much lesser extent, of pivalyl-CoA and isovaleryl-CoA, using radical chemistry (PubMed:22167181). Also exhibits GTPase activity, associated with its G-protein domain (MeaI) that functions as a chaperone that assists cofactor delivery and proper holo-enzyme assembly (PubMed:22167181, PubMed:25675500). The G-domain of IcmF has also a role in its cofactor repair (PubMed:28130442). Does not display ATPase activity.<ref>PMID:22167181</ref> <ref>PMID:25675500</ref> <ref>PMID:28130442</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	G-protein metallochaperones are essential for the proper maturation of numerous metalloenzymes. The G-protein chaperone MMAA in humans (MeaB in bacteria) uses GTP hydrolysis to facilitate the delivery of adenosylcobalamin (AdoCbl) to AdoCbl-dependent methylmalonyl-CoA mutase, an essential metabolic enzyme. This G-protein chaperone also facilitates the removal of damaged cobalamin (Cbl) for repair. Although most chaperones are standalone proteins, isobutyryl-CoA mutase fused (IcmF) has a G-protein domain covalently attached to its target mutase. We previously showed that dimeric MeaB undergoes a 180 degrees rotation to reach a state capable of GTP hydrolysis (an active G-protein state), in which so-called switch III residues of one protomer contact the G-nucleotide of the other protomer. However, it was unclear whether other G-protein chaperones also adopted this conformation. Here, we show that the G-protein domain in a fused system forms a similar active conformation, requiring IcmF oligomerization. IcmF oligomerizes both upon Cbl damage and in the presence of the nonhydrolyzable GTP analog, guanosine-5'-[(beta,gamma)-methyleno]triphosphate, forming supramolecular complexes observable by mass photometry and EM. Cryo-EM structural analysis reveals that the second protomer of the G-protein intermolecular dimer props open the mutase active site using residues of switch III as a wedge, allowing for AdoCbl insertion or damaged Cbl removal. With the series of structural snapshots now available, we now describe here the molecular basis of G-protein-assisted AdoCbl-dependent mutase maturation, explaining how GTP binding prepares a mutase for cofactor delivery and how GTP hydrolysis allows the mutase to capture the cofactor.

-	~~Authors:~~ Vaccaro, ~~F.A.~~, ~~Drennan~~, C.L.	+	Structural insight into G-protein chaperone-mediated maturation of a bacterial adenosylcobalamin-dependent mutase.,Vaccaro FA, Faber DA, Andree GA, Born DA, Kang G, Fonseca DR, Jost M, Drennan CL J Biol Chem. 2023 Sep;299(9):105109. doi: 10.1016/j.jbc.2023.105109. Epub 2023 , Jul 28. PMID:37517695<ref>PMID:37517695</ref>

-	~~Description: Isobutyryl-CoA mutase fused Q341A in~~ the ~~presence~~ of ~~GTP~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Drennan~~, C.L~~]]	+	<div class="pdbe-citations 8ssl" style="background-color:#fffaf0;"></div>
-	[[Category: Vaccaro~~, F.A~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cupriavidus metallidurans CH34]]
		+	[[Category: Large Structures]]
		+	[[Category: Drennan CL]]
		+	[[Category: Vaccaro FA]]

OCA at 06:46, 14 June 2023

OCA — Wed, 14 Jun 2023 06:46:39 GMT

←Older revision		Revision as of 06:46, 14 June 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8ssl is ON HOLD	+	The entry 8ssl is ON HOLD until Paper Publication

	Authors: Vaccaro, F.A., Drennan, C.L.		Authors: Vaccaro, F.A., Drennan, C.L.

OCA: Protected "8ssl" [edit=sysop:move=sysop]

OCA — Thu, 18 May 2023 05:59:47 GMT

Protected "8ssl" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:59, 18 May 2023

OCA: New page: '''Unreleased structure''' The entry 8ssl is ON HOLD Authors: Vaccaro, F.A., Drennan, C.L. Description: Isobutyryl-CoA mutase fused Q341A in the presence of GTP [[Category: Unreleased ...

OCA — Thu, 18 May 2023 05:59:46 GMT

New page: '''Unreleased structure''' The entry 8ssl is ON HOLD Authors: Vaccaro, F.A., Drennan, C.L. Description: Isobutyryl-CoA mutase fused Q341A in the presence of GTP [[Category: Unreleased ...

New page

'''Unreleased structure'''

The entry 8ssl is ON HOLD

Authors: Vaccaro, F.A., Drennan, C.L.

Description: Isobutyryl-CoA mutase fused Q341A in the presence of GTP
[[Category: Unreleased Structures]]
[[Category: Drennan, C.L]]
[[Category: Vaccaro, F.A]]

8ssl - Revision history

OCA at 08:35, 9 May 2024

OCA at 06:46, 14 June 2023

OCA: Protected "8ssl" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8ssl is ON HOLD Authors: Vaccaro, F.A., Drennan, C.L. Description: Isobutyryl-CoA mutase fused Q341A in the presence of GTP [[Category: Unreleased ...