OCA at 07:28, 12 July 2023

OCA — Wed, 12 Jul 2023 07:28:44 GMT

←Older revision		Revision as of 07:28, 12 July 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8st9 is ~~ON HOLD until Paper~~ Publication	+	==Structure of E3 ligase NleL bound to ubiquitin==
		+	<StructureSection load='8st9' size='340' side='right'caption='[[8st9]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8st9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7_str._Sakai Escherichia coli O157:H7 str. Sakai] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ST9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ST9 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AYE:PROP-2-EN-1-AMINE'>AYE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8st9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8st9 OCA], [https://pdbe.org/8st9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8st9 RCSB], [https://www.ebi.ac.uk/pdbsum/8st9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8st9 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0H3JDV8_ECO57 A0A0H3JDV8_ECO57]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	HECT E3 ubiquitin (Ub) ligases direct their modified substrates toward a range of cellular fates dictated by the specific form of monomeric or polymeric Ub (polyUb) signal that is attached. How polyUb specificity is achieved has been a longstanding mystery, despite extensive study ranging from yeast to human. Two outlying examples of bacterial "HECT-like" (bHECT) E3 ligases have been reported in the human pathogens Enterohemorrhagic Escherichia coli and Salmonella Typhimurium, but what parallels can be drawn to eukaryotic HECT (eHECT) mechanism and specificity had not been explored. Here, we expanded the bHECT family and identified catalytically active, bona fide examples in both human and plant pathogens. By determining structures for three bHECT complexes in their primed, Ub-loaded states, we resolved key details of the full bHECT Ub ligation mechanism. One structure provided the first glimpse of a HECT E3 ligase in the act of ligating polyUb, yielding a means to rewire the polyUb specificity of both bHECT and eHECT ligases. Through studying this evolutionarily distinct bHECT family, we have not only gained insight into the function of key bacterial virulence factors but also revealed fundamental principles underlying HECT-type Ub ligation.

-	~~Authors~~:	+	Bacterial mimicry of eukaryotic HECT ubiquitin ligation.,Franklin TG, Brzovic PS, Pruneda JN bioRxiv. 2023 Jun 5:2023.06.05.543783. doi: 10.1101/2023.06.05.543783. Preprint. PMID:37333152<ref>PMID:37333152</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8st9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli O157:H7 str. Sakai]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Franklin TG]]
		+	[[Category: Pruneda JN]]

OCA at 06:46, 14 June 2023

OCA — Wed, 14 Jun 2023 06:46:51 GMT

←Older revision		Revision as of 06:46, 14 June 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8st9 is ON HOLD	+	The entry 8st9 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8st9" [edit=sysop:move=sysop]

OCA — Thu, 18 May 2023 06:00:45 GMT

Protected "8st9" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:00, 18 May 2023

OCA: New page: '''Unreleased structure''' The entry 8st9 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 18 May 2023 06:00:44 GMT

New page: '''Unreleased structure''' The entry 8st9 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8st9 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8st9 - Revision history

OCA at 07:28, 12 July 2023

OCA at 06:46, 14 June 2023

OCA: Protected "8st9" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8st9 is ON HOLD Authors: Description: Category: Unreleased Structures