OCA at 12:42, 26 July 2023

OCA — Wed, 26 Jul 2023 12:42:47 GMT

←Older revision		Revision as of 12:42, 26 July 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8su3 is ~~ON HOLD until Paper~~ Publication	+	==F95S epi-Isozizaene Synthase: complex with 3 Mg2+, inorganic pyrophosphate, and benzyl triethyl ammonium cation==
		+	<StructureSection load='8su3' size='340' side='right'caption='[[8su3]], [[Resolution\|resolution]] 1.29Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8su3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SU3 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.29Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTM:N-BENZYL-N,N-DIETHYLETHANAMINIUM'>BTM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8su3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8su3 OCA], [https://pdbe.org/8su3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8su3 RCSB], [https://www.ebi.ac.uk/pdbsum/8su3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8su3 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CYC1_STRCO CYC1_STRCO] Catalyzes the cyclization of farnesyl diphosphate (FPP) to the sesquiterpene epi-isozizaene.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The class I sesquiterpene cyclase epi-isozizaene synthase from Streptomyces coelicolor (EIZS) catalyzes the transformation of linear farnesyl diphosphate (FPP) into the tricyclic hydrocarbon epi-isozizaene in the biosynthesis of albaflavenone antibiotics. The active site cavity of EIZS is largely framed by four aromatic residues - F95, F96, F198, and W203 - that form a product-shaped contour, serving as a template to chaperone conformations of the flexible substrate and multiple carbocation intermediates leading to epi-isozizaene. Remolding the active site contour by mutagenesis can redirect the cyclization cascade away from epi-isozizaene biosynthesis to generate alternative sesquiterpene products. Here, we present the biochemical and structural characterization of four EIZS mutants in which aromatic residues have been substituted with polar residues (F95S, F96H, F198S, and F198T) to generate alternative cyclization products. Most notably, F95S EIZS generates a mixture of monocyclic sesquiterpene precursors of bisabolane, a D2 diesel fuel substitute. X-ray crystal structures of the characterized mutants reveal subtle changes in the active site contour showing how each aromatic residue influences the chemistry of a different carbocation intermediate in the cyclization cascade. We advance that EIZS may serve as a robust platform for the development of designer cyclases for the generation of high-value sesquiterpene products ranging from pharmaceuticals to biofuels in synthetic biology approaches.

-	~~Authors~~:	+	Reprogramming the Cyclization Cascade of epi-Isozizaene Synthase to Generate Alternative Terpene Products.,Eaton SA, Christianson DW Biochemistry. 2023 Jul 14. doi: 10.1021/acs.biochem.3c00247. PMID:37449555<ref>PMID:37449555</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8su3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Christianson DW]]
		+	[[Category: Eaton SA]]

OCA at 06:47, 14 June 2023

OCA — Wed, 14 Jun 2023 06:47:09 GMT

←Older revision		Revision as of 06:47, 14 June 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8su3 is ON HOLD	+	The entry 8su3 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8su3" [edit=sysop:move=sysop]

OCA — Thu, 18 May 2023 06:02:29 GMT

Protected "8su3" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:02, 18 May 2023

OCA: New page: '''Unreleased structure''' The entry 8su3 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 18 May 2023 06:02:28 GMT

New page: '''Unreleased structure''' The entry 8su3 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8su3 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8su3 - Revision history

OCA at 12:42, 26 July 2023

OCA at 06:47, 14 June 2023

OCA: Protected "8su3" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8su3 is ON HOLD Authors: Description: Category: Unreleased Structures