OCA at 07:02, 27 September 2023

OCA — Wed, 27 Sep 2023 07:02:24 GMT

←Older revision		Revision as of 07:02, 27 September 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8szo is ~~ON HOLD~~	+	==Canavalia villosa lectin in complex with alpha-methyl-mannoside==
		+	<StructureSection load='8szo' size='340' side='right'caption='[[8szo]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8szo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_villosa Canavalia villosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SZO FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8szo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8szo OCA], [https://pdbe.org/8szo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8szo RCSB], [https://www.ebi.ac.uk/pdbsum/8szo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8szo ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	With important carbohydrate binding properties, lectins are proteins able to decipher the glycocode, and as such, they can be used in bioassays involving cell-cell communication, protein targeting, inflammation, and hypernociception, among others. In this study, a new glucose/mannose-specific lectin from Canavalia villosa seeds (Cvill) was isolated by a single affinity chromatography step in a Sephadex((R)) G-50 column, with a purification yield of 19.35mg of lectin per gram of powdered seed. Analysis of intact protein by mass spectrometry showed the lectin is composed of three polypeptide chains, including a 25.6kDa alpha chain, 12.9KDa beta, and 12.6 KDa gamma fragments, similar to the profile of ConA-like glucose/mannose-specific lectins. Partial sequence of the protein was obtained by MS-MALDI TOF/TOF covering 41.7% of its primary structure. Cvill presented sugar specificity to d-glucose, alpha-methyl-d-mannoside, d-mannose, and glycoproteins fetuin and ovoalbumin. The lectin characterization showed that Cvill presents high stability within a broad range of pH and temperature, also showing average toxicity against Artemia nauplii. The proinflammatory effect of Cvill was observed by induction of paw edema and hypernociception in mice, with the participation of the carbohydrate binding site, showing its potential to be used as tool in inflammation studies.

-	~~Authors~~:	+	Lectin from Canavalia villosa seeds: A glucose/mannose-specific protein and a new tool for inflammation studies.,Lossio CF, Moreira CG, Amorim RMF, Nobre CS, Silva MTL, Neto CC, Pinto-Junior VR, Silva IB, Campos J, Assreuy AMS, Cavada BS, Nascimento KS Int J Biol Macromol. 2017 Dec;105(Pt 1):272-280. doi: , 10.1016/j.ijbiomac.2017.07.038. Epub 2017 Jul 8. PMID:28693997<ref>PMID:28693997</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8szo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Canavalia villosa]]
		+	[[Category: Large Structures]]
		+	[[Category: Cavada BS]]
		+	[[Category: Lossio CF]]
		+	[[Category: Nascimento KS]]
		+	[[Category: Neco AHB]]
		+	[[Category: Oliveira MV]]
		+	[[Category: Osterne VJS]]
		+	[[Category: Pinto-Junior VR]]

OCA: Protected "8szo" [edit=sysop:move=sysop]

OCA — Wed, 07 Jun 2023 04:29:02 GMT

Protected "8szo" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:29, 7 June 2023

OCA: New page: '''Unreleased structure''' The entry 8szo is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Jun 2023 04:29:00 GMT

New page: '''Unreleased structure''' The entry 8szo is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8szo is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8szo - Revision history

OCA at 07:02, 27 September 2023

OCA: Protected "8szo" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8szo is ON HOLD Authors: Description: Category: Unreleased Structures