OCA at 11:29, 24 January 2024

OCA — Wed, 24 Jan 2024 11:29:59 GMT

←Older revision		Revision as of 11:29, 24 January 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8t2p is ~~ON HOLD until Paper~~ Publication	+	==5TU-t1 - heterodimeric triplet polymerase ribozyme==
		+	<StructureSection load='8t2p' size='340' side='right'caption='[[8t2p]], [[Resolution\|resolution]] 5.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8t2p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8T2P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8T2P FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 5Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8t2p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8t2p OCA], [https://pdbe.org/8t2p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8t2p RCSB], [https://www.ebi.ac.uk/pdbsum/8t2p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8t2p ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The emergence of an RNA replicase capable of self-replication is considered an important stage in the origin of life. RNA polymerase ribozymes (PR) - including a variant that uses trinucleotide triphosphates (triplets) as substrates - have been created by in vitro evolution and are the closest functional analogues of the replicase, but the structural basis for their function is poorly understood. Here we use single-particle cryogenic electron microscopy (cryo-EM) and high-throughput mutation analysis to obtain the structure of a triplet polymerase ribozyme (TPR) apoenzyme and map its functional landscape. The cryo-EM structure at 5-A resolution reveals the TPR as an RNA heterodimer comprising a catalytic subunit and a noncatalytic, auxiliary subunit, resembling the shape of a left hand with thumb and fingers at a 70 degrees angle. The two subunits are connected by two distinct kissing-loop (KL) interactions that are essential for polymerase function. Our combined structural and functional data suggest a model for templated RNA synthesis by the TPR holoenzyme, whereby heterodimer formation and KL interactions preorganize the TPR for optimal primer-template duplex binding, triplet substrate discrimination, and templated RNA synthesis. These results provide a better understanding of TPR structure and function and should aid the engineering of more efficient PRs.

-	~~Authors~~:	+	Cryo-EM structure and functional landscape of an RNA polymerase ribozyme.,McRae EKS, Wan CJK, Kristoffersen EL, Hansen K, Gianni E, Gallego I, Curran JF, Attwater J, Holliger P, Andersen ES Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2313332121. doi: , 10.1073/pnas.2313332121. Epub 2024 Jan 11. PMID:38207080<ref>PMID:38207080</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8t2p" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Andersen ES]]
		+	[[Category: Gallego I]]
		+	[[Category: Hansen K]]
		+	[[Category: Holliger P]]
		+	[[Category: Kristoffersen E]]
		+	[[Category: McRae EKS]]

OCA at 04:32, 12 July 2023

OCA — Wed, 12 Jul 2023 04:32:46 GMT

←Older revision		Revision as of 04:32, 12 July 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8t2p is ON HOLD	+	The entry 8t2p is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8t2p" [edit=sysop:move=sysop]

OCA — Wed, 14 Jun 2023 06:52:50 GMT

Protected "8t2p" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:52, 14 June 2023

OCA: New page: '''Unreleased structure''' The entry 8t2p is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 14 Jun 2023 06:52:49 GMT

New page: '''Unreleased structure''' The entry 8t2p is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8t2p is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8t2p - Revision history

OCA at 11:29, 24 January 2024

OCA at 04:32, 12 July 2023

OCA: Protected "8t2p" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8t2p is ON HOLD Authors: Description: Category: Unreleased Structures