8t57 - Revision history

OCA at 09:44, 14 February 2024

OCA — Wed, 14 Feb 2024 09:44:07 GMT

←Older revision		Revision as of 09:44, 14 February 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CSCLC_ARATH CSCLC_ARATH] Acts as an osmosensitive calcium-permeable cation channel.		[https://www.uniprot.org/uniprot/CSCLC_ARATH CSCLC_ARATH] Acts as an osmosensitive calcium-permeable cation channel.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Members of the OSCA/TMEM63 family are mechanically activated ion channels and structures of some OSCA members have revealed the architecture of these channels and structural features that are potentially involved in mechanosensation. However, these structures are all in a similar state and information about the motion of different elements of the structure is limited, preventing a deeper understanding of how these channels work. Here, we used cryoelectron microscopy to determine high-resolution structures of Arabidopsis thaliana OSCA1.2 and OSCA2.3 in peptidiscs. The structure of OSCA1.2 matches previous structures of the same protein in different environments. Yet, in OSCA2.3, the TM6a-TM7 linker adopts a different conformation that constricts the pore on its cytoplasmic side. Furthermore, coevolutionary sequence analysis uncovered a conserved interaction between the TM6a-TM7 linker and the beam-like domain (BLD). Our results reveal conformational heterogeneity and differences in conserved interactions between the TMD and BLD among members of the OSCA family.
-
-	Structure of mechanically activated ion channel OSCA2.3 reveals mobile elements in the transmembrane domain.,Jojoa-Cruz S, Burendei B, Lee WH, Ward AB Structure. 2023 Dec 6:S0969-2126(23)00411-2. doi: 10.1016/j.str.2023.11.009. PMID:38103547<ref>PMID:38103547</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8t57" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 23:16, 27 December 2023

OCA — Wed, 27 Dec 2023 23:16:09 GMT

←Older revision		Revision as of 23:16, 27 December 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8t57 is ~~ON HOLD until Paper~~ Publication	+	==Structure of mechanically activated ion channel OSCA2.3 in peptidiscs==
		+	<StructureSection load='8t57' size='340' side='right'caption='[[8t57]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8t57]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8T57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8T57 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8t57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8t57 OCA], [https://pdbe.org/8t57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8t57 RCSB], [https://www.ebi.ac.uk/pdbsum/8t57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8t57 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CSCLC_ARATH CSCLC_ARATH] Acts as an osmosensitive calcium-permeable cation channel.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Members of the OSCA/TMEM63 family are mechanically activated ion channels and structures of some OSCA members have revealed the architecture of these channels and structural features that are potentially involved in mechanosensation. However, these structures are all in a similar state and information about the motion of different elements of the structure is limited, preventing a deeper understanding of how these channels work. Here, we used cryoelectron microscopy to determine high-resolution structures of Arabidopsis thaliana OSCA1.2 and OSCA2.3 in peptidiscs. The structure of OSCA1.2 matches previous structures of the same protein in different environments. Yet, in OSCA2.3, the TM6a-TM7 linker adopts a different conformation that constricts the pore on its cytoplasmic side. Furthermore, coevolutionary sequence analysis uncovered a conserved interaction between the TM6a-TM7 linker and the beam-like domain (BLD). Our results reveal conformational heterogeneity and differences in conserved interactions between the TMD and BLD among members of the OSCA family.

-	~~Authors~~:	+	Structure of mechanically activated ion channel OSCA2.3 reveals mobile elements in the transmembrane domain.,Jojoa-Cruz S, Burendei B, Lee WH, Ward AB Structure. 2023 Dec 6:S0969-2126(23)00411-2. doi: 10.1016/j.str.2023.11.009. PMID:38103547<ref>PMID:38103547</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8t57" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
		+	[[Category: Large Structures]]
		+	[[Category: Burendei B]]
		+	[[Category: Jojoa-Cruz S]]
		+	[[Category: Lee WH]]
		+	[[Category: Ward AB]]

OCA at 11:08, 26 July 2023

OCA — Wed, 26 Jul 2023 11:08:41 GMT

←Older revision		Revision as of 11:08, 26 July 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8t57 is ON HOLD	+	The entry 8t57 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8t57" [edit=sysop:move=sysop]

OCA — Wed, 21 Jun 2023 08:31:51 GMT

Protected "8t57" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:31, 21 June 2023

OCA: New page: '''Unreleased structure''' The entry 8t57 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 21 Jun 2023 08:31:50 GMT

New page: '''Unreleased structure''' The entry 8t57 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8t57 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]