8t6j - Revision history

OCA at 19:57, 29 May 2024

OCA — Wed, 29 May 2024 19:57:25 GMT

←Older revision		Revision as of 19:57, 29 May 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GRM5_HUMAN GRM5_HUMAN] Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.		[https://www.uniprot.org/uniprot/GRM5_HUMAN GRM5_HUMAN] Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metabotropic glutamate receptors belong to a family of G protein-coupled receptors that are obligate dimers and possess a large extracellular ligand-binding domain that is linked via a cysteine-rich domain to their 7-transmembrane domain(1). Upon activation, these receptors undergo a large conformational change to transmit the ligand binding signal from the extracellular ligand-binding domain to the G protein-coupling 7-transmembrane domain(2). In this manuscript, we propose a model for a sequential, multistep activation mechanism of metabotropic glutamate receptor subtype 5. We present a series of structures in lipid nanodiscs, from inactive to fully active, including agonist-bound intermediate states. Further, using bulk and single-molecule fluorescence imaging, we reveal distinct receptor conformations upon allosteric modulator and G protein binding.
		+
		+	Stepwise activation of a metabotropic glutamate receptor.,Krishna Kumar K, Wang H, Habrian C, Latorraca NR, Xu J, O'Brien ES, Zhang C, Montabana E, Koehl A, Marqusee S, Isacoff EY, Kobilka BK Nature. 2024 May;629(8013):951-956. doi: 10.1038/s41586-024-07327-x. Epub 2024 , Apr 17. PMID:38632403<ref>PMID:38632403</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 8t6j" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:01, 21 February 2024

OCA — Wed, 21 Feb 2024 11:01:01 GMT

←Older revision		Revision as of 11:01, 21 February 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GRM5_HUMAN GRM5_HUMAN] Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.		[https://www.uniprot.org/uniprot/GRM5_HUMAN GRM5_HUMAN] Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Metabotropic glutamate receptors belong to a family of G protein-coupled receptors that are obligate dimers and possess a large extracellular ligand-binding domain (ECD) that is linked via a cysteine-rich domain (CRDs) to their 7-transmembrane (TM) domain. Upon activation, these receptors undergo a large conformational change to transmit the ligand binding signal from the ECD to the G protein-coupling TM. In this manuscript, we propose a model for a sequential, multistep activation mechanism of metabotropic glutamate receptor subtype 5. We present a series of structures in lipid nanodiscs, from inactive to fully active, including agonist-bound intermediate states. Further, using bulk and single-molecule fluorescence imaging we reveal distinct receptor conformations upon allosteric modulator and G protein binding.
-
-	Step-wise activation of a Family C GPCR.,Kumar KK, Wang H, Habrian C, Latorraca NR, Xu J, O'Brien ES, Zhang C, Montabana E, Koehl A, Marqusee S, Isacoff EY, Kobilka BK bioRxiv. 2023 Aug 30:2023.08.29.555158. doi: 10.1101/2023.08.29.555158. Preprint. PMID:37693614<ref>PMID:37693614</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8t6j" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:01, 11 October 2023

OCA — Wed, 11 Oct 2023 06:01:01 GMT

←Older revision		Revision as of 06:01, 11 October 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8t6j is ~~ON HOLD until Paper~~ Publication	+	==CDPPB-bound inactive mGlu5==
		+	<StructureSection load='8t6j' size='340' side='right'caption='[[8t6j]], [[Resolution\|resolution]] 3.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8t6j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8T6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8T6J FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=YKU:3-cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl)benzamide'>YKU</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8t6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8t6j OCA], [https://pdbe.org/8t6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8t6j RCSB], [https://www.ebi.ac.uk/pdbsum/8t6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8t6j ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GRM5_HUMAN GRM5_HUMAN] Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metabotropic glutamate receptors belong to a family of G protein-coupled receptors that are obligate dimers and possess a large extracellular ligand-binding domain (ECD) that is linked via a cysteine-rich domain (CRDs) to their 7-transmembrane (TM) domain. Upon activation, these receptors undergo a large conformational change to transmit the ligand binding signal from the ECD to the G protein-coupling TM. In this manuscript, we propose a model for a sequential, multistep activation mechanism of metabotropic glutamate receptor subtype 5. We present a series of structures in lipid nanodiscs, from inactive to fully active, including agonist-bound intermediate states. Further, using bulk and single-molecule fluorescence imaging we reveal distinct receptor conformations upon allosteric modulator and G protein binding.

-	~~Authors: Krishna Kumar, K~~., ~~Wang~~, H., ~~Kobilka~~, B.K.	+	Step-wise activation of a Family C GPCR.,Kumar KK, Wang H, Habrian C, Latorraca NR, Xu J, O'Brien ES, Zhang C, Montabana E, Koehl A, Marqusee S, Isacoff EY, Kobilka BK bioRxiv. 2023 Aug 30:2023.08.29.555158. doi: 10.1101/2023.08.29.555158. Preprint. PMID:37693614<ref>PMID:37693614</ref>

-	~~Description: CDPPB~~-~~bound inactive mGlu5~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Krishna Kumar, K~~]]	+	<div class="pdbe-citations 8t6j" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Wang, H~~]]	+	== References ==
-	[[Category: ~~Kobilka, B.~~K]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Kobilka BK]]
		+	[[Category: Krishna Kumar K]]
		+	[[Category: Wang H]]

OCA at 12:23, 20 September 2023

OCA — Wed, 20 Sep 2023 12:23:42 GMT

←Older revision		Revision as of 12:23, 20 September 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8t6j is ON HOLD	+	The entry 8t6j is ON HOLD until Paper Publication

	Authors: Krishna Kumar, K., Wang, H., Kobilka, B.K.		Authors: Krishna Kumar, K., Wang, H., Kobilka, B.K.

OCA: Protected "8t6j" [edit=sysop:move=sysop]

OCA — Wed, 28 Jun 2023 20:03:26 GMT

Protected "8t6j" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:03, 28 June 2023

OCA: New page: '''Unreleased structure''' The entry 8t6j is ON HOLD Authors: Krishna Kumar, K., Wang, H., Kobilka, B.K. Description: CDPPB-bound inactive mGlu5 Category: Unreleased Structures [[C...

OCA — Wed, 28 Jun 2023 20:03:25 GMT

New page: '''Unreleased structure''' The entry 8t6j is ON HOLD Authors: Krishna Kumar, K., Wang, H., Kobilka, B.K. Description: CDPPB-bound inactive mGlu5 Category: Unreleased Structures [[C...

New page

'''Unreleased structure'''

The entry 8t6j is ON HOLD

Authors: Krishna Kumar, K., Wang, H., Kobilka, B.K.

Description: CDPPB-bound inactive mGlu5
[[Category: Unreleased Structures]]
[[Category: Krishna Kumar, K]]
[[Category: Wang, H]]
[[Category: Kobilka, B.K]]