OCA at 08:32, 16 August 2023

OCA — Wed, 16 Aug 2023 08:32:01 GMT

←Older revision		Revision as of 08:32, 16 August 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 8tfr is ~~ON HOLD~~	+	==Apo Fab from C10-S66K antibody==
		+	<StructureSection load='8tfr' size='340' side='right'caption='[[8tfr]], [[Resolution\|resolution]] 2.99Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8tfr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Streptococcus_sp._'group_G' Streptococcus sp. 'group G']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TFR FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.99Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tfr OCA], [https://pdbe.org/8tfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tfr RCSB], [https://www.ebi.ac.uk/pdbsum/8tfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tfr ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPG2_STRSG SPG2_STRSG]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The opioid overdose crisis primarily driven by potent synthetic opioids resulted in more than 500,000 deaths in the US over the last 20 years. Though naloxone, a short-acting medication, remains the primary treatment option for temporarily reversing opioid overdose effects, alternative countermeasures are needed. Monoclonal antibodies present a versatile therapeutic opportunity that can be tailored to synthetic opioids and help prevent post-treatment renarcotization. The ultrapotent analog carfentanil is especially concerning due to its unique pharmacological properties. With this in mind, we generated a fully human antibody through a drug-specific B cell sorting strategy with a combination of carfentanil and fentanyl probes. The resulting pan-specific antibody was further optimized through scFv phage display, producing C10-S66K. This monoclonal antibody displays high affinity to carfentanil, fentanyl, and other analogs and reversed carfentanil-induced respiratory depression. Additionally, X-ray crystal structures with carfentanil and fentanyl bound provided structural insight into key drug:antibody interactions.

-	~~Authors: Pholcharee~~, T., Wilson, I.A.	+	An Engineered Human-Antibody Fragment with Fentanyl Pan-Specificity That Reverses Carfentanil-Induced Respiratory Depression.,Eubanks LM, Pholcharee T, Oyen D, Natori Y, Zhou B, Wilson IA, Janda KD ACS Chem Neurosci. 2023 Aug 3. doi: 10.1021/acschemneuro.3c00455. PMID:37534714<ref>PMID:37534714</ref>

-	~~Description: Apo Fab from C10~~-~~S66K antibody~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Wilson, I~~.A]]	+	<div class="pdbe-citations 8tfr" style="background-color:#fffaf0;"></div>
-	[[Category: Pholcharee, T]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Streptococcus sp. 'group G']]
		+	[[Category: Pholcharee T]]
		+	[[Category: Wilson IA]]

OCA: Protected "8tfr" [edit=sysop:move=sysop]

OCA — Wed, 19 Jul 2023 05:04:46 GMT

Protected "8tfr" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:04, 19 July 2023

OCA: New page: '''Unreleased structure''' The entry 8tfr is ON HOLD Authors: Pholcharee, T., Wilson, I.A. Description: Apo Fab from C10-S66K antibody Category: Unreleased Structures [[Category: W...

OCA — Wed, 19 Jul 2023 05:04:45 GMT

New page: '''Unreleased structure''' The entry 8tfr is ON HOLD Authors: Pholcharee, T., Wilson, I.A. Description: Apo Fab from C10-S66K antibody Category: Unreleased Structures [[Category: W...

New page

'''Unreleased structure'''

The entry 8tfr is ON HOLD

Authors: Pholcharee, T., Wilson, I.A.

Description: Apo Fab from C10-S66K antibody
[[Category: Unreleased Structures]]
[[Category: Wilson, I.A]]
[[Category: Pholcharee, T]]

8tfr - Revision history

OCA at 08:32, 16 August 2023

OCA: Protected "8tfr" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8tfr is ON HOLD Authors: Pholcharee, T., Wilson, I.A. Description: Apo Fab from C10-S66K antibody Category: Unreleased Structures [[Category: W...