OCA at 10:10, 17 January 2024

OCA — Wed, 17 Jan 2024 10:10:07 GMT

←Older revision		Revision as of 10:10, 17 January 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8thn is ~~ON HOLD until 2025~~-07-17	+	==KcsA M96V mutant with Y78ester in High K+==
		+	<StructureSection load='8thn' size='340' side='right'caption='[[8thn]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8thn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8THN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8THN FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=TBA:TETRABUTYLAMMONIUM+ION'>TBA</scene>, <scene name='pdbligand=TYF:(2S)-2-HYDROXY-3-(4-HYDROXYPHENYL)PROPANOIC+ACID'>TYF</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8thn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8thn OCA], [https://pdbe.org/8thn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8thn RCSB], [https://www.ebi.ac.uk/pdbsum/8thn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8thn ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/KCSA_STRLI KCSA_STRLI] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).<ref>PMID:7489706</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Amide-to-ester substitutions are used to study the role of the amide bonds of the protein backbone in protein structure, function, and folding. An amber suppressor tRNA/synthetase pair has been reported for incorporation of p-hydroxy-phenyl-L-lactic acid (HPLA), thereby introducing ester substitution at tyrosine residues. However, the application of this approach was limited due to the low yields of the modified proteins and the high cost of HPLA. Here we report the in vivo generation of HPLA from the significantly cheaper phenyl-L-lactic acid. We also construct an optimized plasmid with the HPLA suppressor tRNA/synthetase pair that provides higher yields of the modified proteins. The combination of the new plasmid and the in-situ generation of HPLA provides a facile and economical approach for introducing tyrosine ester substitutions. We demonstrate the utility of this approach by introducing tyrosine ester substitutions into the K(+) channel KcsA and the integral membrane enzyme GlpG. We introduce the tyrosine ester in the selectivity filter of the M96V mutant of the KcsA to probe the role of the second ion binding site in the conformation of the selectivity filter and the process of inactivation. We use tyrosine ester substitutions in GlpG to perturb backbone H-bonds to investigate the contribution of these H-bonds to membrane protein stability. We anticipate that the approach developed in this study will facilitate further investigations using tyrosine ester substitutions.

-	~~Authors~~:	+	A facile approach for incorporating tyrosine esters to probe ion-binding sites and backbone hydrogen bonds.,Reddi R, Chatterjee S, Matulef K, Gustafson A, Gao L, Valiyaveetil FI J Biol Chem. 2023 Nov 30;300(1):105517. doi: 10.1016/j.jbc.2023.105517. PMID:38042487<ref>PMID:38042487</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8thn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
		+	[[Category: Streptomyces lividans]]
		+	[[Category: Reddi R]]
		+	[[Category: Valiyaveetil FI]]

OCA: Protected "8thn" [edit=sysop:move=sysop]

OCA — Wed, 26 Jul 2023 11:13:43 GMT

Protected "8thn" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:13, 26 July 2023

OCA: New page: '''Unreleased structure''' The entry 8thn is ON HOLD until 2025-07-17 Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Jul 2023 11:13:42 GMT

New page: '''Unreleased structure''' The entry 8thn is ON HOLD until 2025-07-17 Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8thn is ON HOLD until 2025-07-17

Authors:

Description:
[[Category: Unreleased Structures]]

8thn - Revision history

OCA at 10:10, 17 January 2024

OCA: Protected "8thn" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8thn is ON HOLD until 2025-07-17 Authors: Description: Category: Unreleased Structures