8tli - Revision history

OCA at 01:02, 28 December 2023

OCA — Thu, 28 Dec 2023 01:02:40 GMT

←Older revision		Revision as of 01:02, 28 December 2023
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	==THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)==		==THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)==
-	<StructureSection load='8tli' size='340' side='right' caption='[[8tli]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='8tli' size='340' side='right'caption='[[8tli]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[8tli]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TLI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=8TLI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[8tli]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TLI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tlx\|1tlx]], [[2tlx\|2tlx]], [[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tli OCA], [https://pdbe.org/8tli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tli RCSB], [https://www.ebi.ac.uk/pdbsum/8tli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tli ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=8tli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tli OCA], [~~http~~://pdbe.org/8tli PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=8tli RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/8tli PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=8tli ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/THER_BACTH THER_BACTH]] Extracellular zinc metalloprotease.	+	[https://www.uniprot.org/uniprot/THER_BACTH THER_BACTH] Extracellular zinc metalloprotease.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/8tli_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/8tli_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 31:

	==See Also==		==See Also==
-	*[[Thermolysin\|Thermolysin]]	+	*[[Thermolysin 3D structures\|Thermolysin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus thermoproteolyticus]]		[[Category: Bacillus thermoproteolyticus]]
-	[[Category: ~~Thermolysin~~]]	+	[[Category: Large Structures]]
-	[[Category: Done~~, S H~~]]	+	[[Category: Done SH]]
-	[[Category: English~~, A C~~]]	+	[[Category: English AC]]
-	[[Category: Groom~~, C R~~]]	+	[[Category: Groom CR]]
-	[[Category: Hubbard~~, R E]]~~	+	[[Category: Hubbard RE]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Metalloproteinase]]~~	+
-	~~[[Category: Organic solvent~~]]	+

OCA at 05:34, 5 August 2016

OCA — Fri, 05 Aug 2016 05:34:30 GMT

←Older revision		Revision as of 05:34, 5 August 2016
Line 1:		Line 1:
		+
	==THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)==		==THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)==
	<StructureSection load='8tli' size='340' side='right' caption='[[8tli]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='8tli' size='340' side='right' caption='[[8tli]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tlx\|1tlx]], [[2tlx\|2tlx]], [[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tlx\|1tlx]], [[2tlx\|2tlx]], [[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8tli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=8tli RCSB], [http://www.ebi.ac.uk/pdbsum/8tli PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8tli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tli OCA], [http://pdbe.org/8tli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=8tli RCSB], [http://www.ebi.ac.uk/pdbsum/8tli PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=8tli ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=8tli ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 8tli" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 16:10, 25 December 2014

OCA — Thu, 25 Dec 2014 16:10:06 GMT

←Older revision		Revision as of 16:10, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8tli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=8tli RCSB], [http://www.ebi.ac.uk/pdbsum/8tli PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8tli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=8tli RCSB], [http://www.ebi.ac.uk/pdbsum/8tli PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/THER_BACTH THER_BACTH]] Extracellular zinc metalloprotease.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:25, 18 December 2014

OCA — Thu, 18 Dec 2014 08:25:29 GMT

←Older revision		Revision as of 08:25, 18 December 2014
Line 1:		Line 1:
-	[[~~Image:~~8tli.~~png~~\|~~left~~\|200px]]	+	==THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)==
		+	<StructureSection load='8tli' size='340' side='right' caption='[[8tli]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8tli]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8TLI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tlx\|1tlx]], [[2tlx\|2tlx]], [[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8tli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=8tli RCSB], [http://www.ebi.ac.uk/pdbsum/8tli PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/8tli_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multiple-solvent crystal structure determination (MSCS) allows the position and orientation of bound solvent fragments to be identified by determining the structure of protein crystals soaked in organic solvents. We have extended this technique by the determination of high-resolution crystal structures of thermolysin (TLN), generated from crystals soaked in 2% to 100% isopropanol. The procedure causes only minor changes to the conformation of the protein, and an increasing number of isopropanol interaction sites could be identified as the solvent concentration is increased. Isopropanol occupies all four of the main subsites in the active site, although this was only observed at very high concentrations of isopropanol for three of the four subsites. Analysis of the isopropanol positions shows little correlation with interaction energy computed using a molecular mechanics force field, but the experimentally determined positions of isopropanol are consistent with the structures of known protein-ligand complexes of TLN.

-	~~{{STRUCTURE_8tli\| PDB=8tli \| SCENE= }}~~	+	Locating interaction sites on proteins: the crystal structure of thermolysin soaked in 2% to 100% isopropanol.,English AC, Done SH, Caves LS, Groom CR, Hubbard RE Proteins. 1999 Dec 1;37(4):628-40. PMID:10651278<ref>PMID:10651278</ref>

-	~~===THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10651278}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[8tli]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=8TLI OCA].~~	+

	==See Also==		==See Also==
	*[[Thermolysin\|Thermolysin]]		*[[Thermolysin\|Thermolysin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010651278<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus thermoproteolyticus]]		[[Category: Bacillus thermoproteolyticus]]
	[[Category: Thermolysin]]		[[Category: Thermolysin]]
-	[[Category: Done, S H.]]	+	[[Category: Done, S H]]
-	[[Category: English, A C.]]	+	[[Category: English, A C]]
-	[[Category: Groom, C R.]]	+	[[Category: Groom, C R]]
-	[[Category: Hubbard, R E.]]	+	[[Category: Hubbard, R E]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Metalloproteinase]]		[[Category: Metalloproteinase]]
	[[Category: Organic solvent]]		[[Category: Organic solvent]]

OCA at 18:44, 20 October 2012

OCA — Sat, 20 Oct 2012 18:44:46 GMT

←Older revision		Revision as of 18:44, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[8tli]] is a 1 chain structure ~~of [[Thermolysin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TLI OCA].	+	[[8tli]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TLI OCA].

	==See Also==		==See Also==

OCA at 05:05, 27 July 2012

OCA — Fri, 27 Jul 2012 05:05:57 GMT

←Older revision		Revision as of 05:05, 27 July 2012
Line 1:		Line 1:
	[[Image:8tli.png\|left\|200px]]		[[Image:8tli.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_8tli", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_8tli\| PDB=8tli \| SCENE= }}		{{STRUCTURE_8tli\| PDB=8tli \| SCENE= }}

	===THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)===		===THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10651278}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10651278 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_10651278}}		{{ABSTRACT_PUBMED_10651278}}

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	==See Also==		==See Also==
-	*[[Thermolysin]]	+	*[[Thermolysin\|Thermolysin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10651278~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010651278</ref><references group="xtra"/>
	[[Category: Bacillus thermoproteolyticus]]		[[Category: Bacillus thermoproteolyticus]]
	[[Category: Thermolysin]]		[[Category: Thermolysin]]

OCA: Protected "8tli" [edit=sysop:move=sysop]

OCA — Sat, 25 Dec 2010 18:02:50 GMT

Protected "8tli" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:02, 25 December 2010

OCA at 18:02, 25 December 2010

OCA — Sat, 25 Dec 2010 18:02:49 GMT

←Older revision		Revision as of 18:02, 25 December 2010
Line 1:		Line 1:
-	{{Seed}}
	[[Image:8tli.png\|left\|200px]]		[[Image:8tli.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~8TLI~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TLI OCA].	+	[[8tli]] is a 1 chain structure of [[Thermolysin]] with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TLI OCA].
		+
		+	==See Also==
		+	*[[Thermolysin]]

	==Reference==		==Reference==
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	[[Category: Metalloproteinase]]		[[Category: Metalloproteinase]]
	[[Category: Organic solvent]]		[[Category: Organic solvent]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:22:57 2009''

OCA at 19:22, 17 February 2009

OCA — Tue, 17 Feb 2009 19:22:57 GMT

←Older revision		Revision as of 19:22, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	8TLI is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TLI OCA].	+	8TLI is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TLI OCA].

	==Reference==		==Reference==
-	~~Locating interaction sites on proteins: the crystal structure of thermolysin soaked in 2% to 100% isopropanol., English AC, Done SH, Caves LS, Groom CR, Hubbard RE, Proteins. 1999 Dec 1;37(4):628-40.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/10651278 10651278]~~	+	<ref group="xtra">PMID:10651278</ref><references group="xtra"/>
	[[Category: Bacillus thermoproteolyticus]]		[[Category: Bacillus thermoproteolyticus]]
-	[[Category: Single protein]]
	[[Category: Thermolysin]]		[[Category: Thermolysin]]
	[[Category: Done, S H.]]		[[Category: Done, S H.]]
Line 35:		Line 34:
	[[Category: Organic solvent]]		[[Category: Organic solvent]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri Jul 11 12~~:49:~~08 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:22:57 2009''

OCA at 09:49, 11 July 2008

OCA — Fri, 11 Jul 2008 09:49:09 GMT

←Older revision		Revision as of 09:49, 11 July 2008
Line 1:		Line 1:
-	[[Image:8tli.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:8tli.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_8tli\| PDB=8tli \| SCENE= }}		{{STRUCTURE_8tli\| PDB=8tli \| SCENE= }}

-	~~'''~~THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)~~'''~~	+	===THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)===


-	~~==Overview==~~	+	<!--
-	~~Multiple-solvent crystal structure determination (MSCS) allows~~ the ~~position and orientation of bound solvent fragments~~ to ~~be identified by determining~~ the ~~structure of protein crystals soaked in organic solvents~~. ~~We have extended this technique by the determination of high-resolution crystal structures of thermolysin (TLN~~), ~~generated from crystals soaked in 2% to 100% isopropanol. The procedure causes only minor changes to~~ the ~~conformation of the protein, and an increasing~~ number ~~of isopropanol interaction sites could be identified as the solvent concentration is increased~~. Isopropanol occupies all four of the main subsites in the active site, although this was only observed at very high concentrations of isopropanol for three of the four subsites. Analysis of the isopropanol positions shows little correlation with interaction energy computed using a molecular mechanics force field, but the experimentally determined positions of isopropanol are consistent with the structures of known protein-~~ligand complexes of TLN.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_10651278}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 10651278 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_10651278}}

	==About this Structure==		==About this Structure==
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	[[Category: Metalloproteinase]]		[[Category: Metalloproteinase]]
	[[Category: Organic solvent]]		[[Category: Organic solvent]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 22~~:52:04 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Jul 11 12:49:08 2008''