OCA at 08:06, 6 December 2023

OCA — Wed, 06 Dec 2023 08:06:32 GMT

←Older revision		Revision as of 08:06, 6 December 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8uhf is ~~ON HOLD~~	+	==Cryo-EM of Vibrio cholerae toxin co-regulated pilus - asymmetric reconstruction==
		+	<StructureSection load='8uhf' size='340' side='right'caption='[[8uhf]], [[Resolution\|resolution]] 3.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8uhf]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8UHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8UHF FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.8Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8uhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8uhf OCA], [https://pdbe.org/8uhf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8uhf RCSB], [https://www.ebi.ac.uk/pdbsum/8uhf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8uhf ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q93TT5_VIBCL Q93TT5_VIBCL]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type IV pili (T4P) are ubiquitous in both bacteria and archaea. They are polymers of the major pilin protein, which has an extended and protruding N-terminal helix, alpha1, and a globular C-terminal domain. Cryo-EM structures have revealed key differences between the bacterial and archaeal T4P in their C-terminal domain structure and in the packing and continuity of alpha1. This segment forms a continuous alpha-helix in archaeal T4P but is partially melted in all published bacterial T4P structures due to a conserved helix breaking proline at position 22. The tad (tight adhesion) T4P are found in both bacteria and archaea and are thought to have been acquired by bacteria through horizontal transfer from archaea. Tad pilins are unique among the T4 pilins, being only 40 to 60 residues in length and entirely lacking a C-terminal domain. They also lack the Pro22 found in all high-resolution bacterial T4P structures. We show using cryo-EM that the bacterial tad pilus from Caulobacter crescentus is composed of continuous helical subunits that, like the archaeal pilins, lack the melted portion seen in other bacterial T4P and share the packing arrangement of the archaeal T4P. We further show that a bacterial T4P, the Vibrio cholerae toxin coregulated pilus, which lacks Pro22 but is not in the tad family, has a continuous N-terminal alpha-helix, yet its alpha1 s are arranged similar to those in other bacterial T4P. Our results highlight the role of Pro22 in helix melting and support an evolutionary relationship between tad and archaeal T4P.

-	~~Authors:~~ Sonani, ~~R.R.~~, ~~Kundra~~, S., ~~Craig~~, L., Egelman, E.H.	+	Tad and toxin-coregulated pilus structures reveal unexpected diversity in bacterial type IV pili.,Sonani RR, Sanchez JC, Baumgardt JK, Kundra S, Wright ER, Craig L, Egelman EH Proc Natl Acad Sci U S A. 2023 Dec 5;120(49):e2316668120. doi: , 10.1073/pnas.2316668120. Epub 2023 Nov 27. PMID:38011558<ref>PMID:38011558</ref>

-	~~Description: Cryo-EM~~ of ~~Vibrio cholerae toxin co~~-~~regulated pilus~~ -~~asymmetric reconstruction~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Sonani, R.R~~]]	+	<div class="pdbe-citations 8uhf" style="background-color:#fffaf0;"></div>
-	[[Category: Egelman~~, E.H~~]]	+	== References ==
-	[[Category: Kundra, S]]	+	<references/>
-	[[Category: ~~Craig, L~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Vibrio cholerae]]
		+	[[Category: Craig L]]
		+	[[Category: Egelman EH]]
		+	[[Category: Kundra S]]
		+	[[Category: Sonani RR]]

OCA: Protected "8uhf" [edit=sysop:move=sysop]

OCA — Wed, 18 Oct 2023 12:28:54 GMT

Protected "8uhf" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:28, 18 October 2023

OCA: New page: '''Unreleased structure''' The entry 8uhf is ON HOLD Authors: Sonani, R.R., Kundra, S., Craig, L., Egelman, E.H. Description: Cryo-EM of Vibrio cholerae toxin co-regulated pilus -asymm...

OCA — Wed, 18 Oct 2023 12:28:52 GMT

New page: '''Unreleased structure''' The entry 8uhf is ON HOLD Authors: Sonani, R.R., Kundra, S., Craig, L., Egelman, E.H. Description: Cryo-EM of Vibrio cholerae toxin co-regulated pilus -asymm...

New page

'''Unreleased structure'''

The entry 8uhf is ON HOLD

Authors: Sonani, R.R., Kundra, S., Craig, L., Egelman, E.H.

Description: Cryo-EM of Vibrio cholerae toxin co-regulated pilus -asymmetric reconstruction
[[Category: Unreleased Structures]]
[[Category: Sonani, R.R]]
[[Category: Egelman, E.H]]
[[Category: Kundra, S]]
[[Category: Craig, L]]

8uhf - Revision history

OCA at 08:06, 6 December 2023

OCA: Protected "8uhf" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8uhf is ON HOLD Authors: Sonani, R.R., Kundra, S., Craig, L., Egelman, E.H. Description: Cryo-EM of Vibrio cholerae toxin co-regulated pilus -asymm...