OCA at 05:51, 3 April 2024

OCA — Wed, 03 Apr 2024 05:51:27 GMT

←Older revision		Revision as of 05:51, 3 April 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8uob is ~~ON HOLD until Paper Publication~~	+	==SARS-CoV-2 Papain-like protease (PLpro) with Inhibitor Jun12682==
-		+	<StructureSection load='8uob' size='340' side='right'caption='[[8uob]], [[Resolution\|resolution]] 2.52Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[8uob]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8UOB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8UOB FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.52Å</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=XB5:5-[2-(dimethylamino)ethoxy]-N-{(1R)-1-[(3M,5P)-3-(1-ethyl-1H-pyrazol-3-yl)-5-(1-methyl-1H-pyrazol-4-yl)phenyl]ethyl}-2-methylbenzamide'>XB5</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8uob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8uob OCA], [https://pdbe.org/8uob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8uob RCSB], [https://www.ebi.ac.uk/pdbsum/8uob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8uob ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/R1A_SARS2 R1A_SARS2] Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.[UniProtKB:P0C6X7] Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.[UniProtKB:P0C6X7] May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.[UniProtKB:P0C6X7] Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.[UniProtKB:P0C6X7] Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.[UniProtKB:P0C6X7] Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).[UniProtKB:P0C6X7] Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] May participate in viral replication by acting as a ssRNA-binding protein.[UniProtKB:P0C6X7] Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.[UniProtKB:P0C6X7]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
		+	[[Category: Ansari A]]
		+	[[Category: Arnold E]]
		+	[[Category: Ruiz FX]]
		+	[[Category: Tan B]]
		+	[[Category: Wang J]]

OCA at 10:31, 6 March 2024

OCA — Wed, 06 Mar 2024 10:31:15 GMT

←Older revision		Revision as of 10:31, 6 March 2024
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8uob is ON HOLD	+	The entry 8uob is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 08:10, 1 March 2024

OCA — Fri, 01 Mar 2024 08:10:10 GMT

←Older revision		Revision as of 08:10, 1 March 2024
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8uob is ON HOLD ~~until Paper Publication~~	+	The entry 8uob is ON HOLD

	Authors:		Authors:

OCA at 08:55, 20 December 2023

OCA — Wed, 20 Dec 2023 08:55:35 GMT

←Older revision		Revision as of 08:55, 20 December 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8uob is ON HOLD	+	The entry 8uob is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8uob" [edit=sysop:move=sysop]

OCA — Wed, 25 Oct 2023 06:04:20 GMT

Protected "8uob" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:04, 25 October 2023

OCA: New page: '''Unreleased structure''' The entry 8uob is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 25 Oct 2023 06:04:19 GMT

New page: '''Unreleased structure''' The entry 8uob is ON HOLD Authors: Description: Category: Unreleased Structures

New page