OCA at 10:11, 17 January 2024

OCA — Wed, 17 Jan 2024 10:11:06 GMT

←Older revision		Revision as of 10:11, 17 January 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8v0f is ~~ON HOLD until Paper~~ Publication	+	==Cryo-EM structure of the unliganded hexameric prenyltransferase in bifunctional copalyl diphosphate synthase from Penicillium fellutanum with an open conformation==
		+	<StructureSection load='8v0f' size='340' side='right'caption='[[8v0f]], [[Resolution\|resolution]] 2.81Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8v0f]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium_fellutanum_ATCC_48694 Penicillium fellutanum ATCC 48694]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8V0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8V0F FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.81Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8v0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8v0f OCA], [https://pdbe.org/8v0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8v0f RCSB], [https://www.ebi.ac.uk/pdbsum/8v0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8v0f ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Copalyl diphosphate synthase from Penicillium fellutanum (PfCPS) is an assembly-line terpene synthase that contains both prenyltransferase and class II cyclase activities. The prenyltransferase catalyzes processive chain elongation reactions using dimethylallyl diphosphate and three equivalents of isopentenyl diphosphate to yield geranylgeranyl diphosphate, which is then utilized as a substrate by the class II cyclase domain to generate copalyl diphosphate. Here, we report the 2.81 A-resolution cryo-EM structure of the hexameric prenyltransferase of full-length PfCPS, which is surrounded by randomly splayed-out class II cyclase domains connected by disordered polypeptide linkers. The hexamer can be described as a trimer of dimers; surprisingly, one of the three dimer-dimer interfaces is separated to yield an open hexamer conformation, thus breaking the D3 symmetry typically observed in crystal structures of other prenyltransferase hexamers such as wild-type human GGPP synthase (hGGPPS). Interestingly, however, an open hexamer conformation was previously observed in the crystal structure of D188Y hGGPPS, apparently facilitated by hexamer-hexamer packing in the crystal lattice. The cryo-EM structure of the PfCPS prenyltransferase hexamer is the first to reveal that an open conformation can be achieved even in the absence of a point mutation or interaction with another hexamer. Even though PfCPS octamers are not detected, we suggest that the open hexamer conformation represents an intermediate in the hexamer-octamer equilibrium for those prenyltransferases that do exhibit oligomeric heterogeneity.

-	~~Authors~~:	+	Structure of the prenyltransferase in bifunctional copalyl diphosphate synthase from Penicillium fellutanum reveals an open hexamer conformation.,Gaynes MN, Ronnebaum TA, Schultz K, Faylo JL, Marmorstein R, Christianson DW J Struct Biol. 2024 Jan 5;216(1):108060. doi: 10.1016/j.jsb.2023.108060. PMID:38184156<ref>PMID:38184156</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8v0f" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Penicillium fellutanum ATCC 48694]]
		+	[[Category: Christianson DW]]
		+	[[Category: Gaynes MN]]

OCA at 22:12, 27 December 2023

OCA — Wed, 27 Dec 2023 22:12:43 GMT

←Older revision		Revision as of 22:12, 27 December 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8v0f is ON HOLD	+	The entry 8v0f is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8v0f" [edit=sysop:move=sysop]

OCA — Wed, 29 Nov 2023 08:36:17 GMT

Protected "8v0f" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:36, 29 November 2023

OCA: New page: '''Unreleased structure''' The entry 8v0f is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Nov 2023 08:36:12 GMT

New page: '''Unreleased structure''' The entry 8v0f is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8v0f is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8v0f - Revision history

OCA at 10:11, 17 January 2024

OCA at 22:12, 27 December 2023

OCA: Protected "8v0f" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8v0f is ON HOLD Authors: Description: Category: Unreleased Structures