8waf - Revision history

OCA at 08:13, 10 April 2024

OCA — Wed, 10 Apr 2024 08:13:52 GMT

←Older revision		Revision as of 08:13, 10 April 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CHUP1_ARATH CHUP1_ARATH] Required for the positioning and movement of chloroplasts. Interacts with profilin and actin independent of its polymerization status. Regulates chloroplast localization by anchoring chloroplasts to the plasma membrane and forming a bridge to the actin cytoskeleton.<ref>PMID:12490952</ref> <ref>PMID:14615600</ref> <ref>PMID:18193273</ref> <ref>PMID:18715957</ref>		[https://www.uniprot.org/uniprot/CHUP1_ARATH CHUP1_ARATH] Required for the positioning and movement of chloroplasts. Interacts with profilin and actin independent of its polymerization status. Regulates chloroplast localization by anchoring chloroplasts to the plasma membrane and forming a bridge to the actin cytoskeleton.<ref>PMID:12490952</ref> <ref>PMID:14615600</ref> <ref>PMID:18193273</ref> <ref>PMID:18715957</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Plants have unique responses to fluctuating light conditions. One such response involves chloroplast photorelocation movement, which optimizes photosynthesis under weak light by the accumulation of chloroplasts along the periclinal side of the cell, which prevents photodamage under strong light by avoiding chloroplast positioning toward the anticlinal side of the cell. This light-responsive chloroplast movement relies on the reorganization of chloroplast actin (cp-actin) filaments. Previous studies have suggested that CHLOROPLAST UNUSUAL POSITIONING 1 (CHUP1) is essential for chloroplast photorelocation movement as a regulator of cp-actin filaments. In this study, we conducted comprehensive analyses to understand CHUP1 function. Functional, fluorescently-tagged CHUP1 colocalized with and was coordinately reorganized with cp-actin filaments on the chloroplast outer envelope during chloroplast movement in Arabidopsis thaliana. CHUP1 distribution was reversibly regulated in a blue light- and phototropin-dependent manner. X-ray crystallography revealed that the CHUP1 C-terminal domain shares structural homology with the formin homology 2 (FH2) domain, despite lacking sequence similarity. Furthermore, the CHUP1 C-terminal domain promoted actin polymerization in the presence of profilin in vitro. Taken together, our findings indicate that CHUP1 is a plant-specific actin polymerization factor that has convergently evolved to assemble cp-actin filaments and enables chloroplast photorelocation movement.
-
-	CHLOROPLAST UNUSUAL POSITIONING 1 is a plant-specific actin polymerization factor regulating chloroplast movement.,Kong SG, Yamazaki Y, Shimada A, T Kijima S, Hirose K, Katoh K, Ahn J, Song HG, Han JW, Higa T, Takano A, Nakamura Y, Suetsugu N, Kohda D, Uyeda TQP, Wada M Plant Cell. 2023 Dec 22:koad320. doi: 10.1093/plcell/koad320. PMID:38134410<ref>PMID:38134410</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 8waf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:11, 17 January 2024

OCA — Wed, 17 Jan 2024 10:11:38 GMT

←Older revision		Revision as of 10:11, 17 January 2024
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 8waf is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the C-terminal fragment (residues 756-982 with the C864S mutation) of Arabidopsis thaliana CHUP1==
		+	<StructureSection load='8waf' size='340' side='right'caption='[[8waf]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8waf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WAF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WAF FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8waf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8waf OCA], [https://pdbe.org/8waf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8waf RCSB], [https://www.ebi.ac.uk/pdbsum/8waf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8waf ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CHUP1_ARATH CHUP1_ARATH] Required for the positioning and movement of chloroplasts. Interacts with profilin and actin independent of its polymerization status. Regulates chloroplast localization by anchoring chloroplasts to the plasma membrane and forming a bridge to the actin cytoskeleton.<ref>PMID:12490952</ref> <ref>PMID:14615600</ref> <ref>PMID:18193273</ref> <ref>PMID:18715957</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Plants have unique responses to fluctuating light conditions. One such response involves chloroplast photorelocation movement, which optimizes photosynthesis under weak light by the accumulation of chloroplasts along the periclinal side of the cell, which prevents photodamage under strong light by avoiding chloroplast positioning toward the anticlinal side of the cell. This light-responsive chloroplast movement relies on the reorganization of chloroplast actin (cp-actin) filaments. Previous studies have suggested that CHLOROPLAST UNUSUAL POSITIONING 1 (CHUP1) is essential for chloroplast photorelocation movement as a regulator of cp-actin filaments. In this study, we conducted comprehensive analyses to understand CHUP1 function. Functional, fluorescently-tagged CHUP1 colocalized with and was coordinately reorganized with cp-actin filaments on the chloroplast outer envelope during chloroplast movement in Arabidopsis thaliana. CHUP1 distribution was reversibly regulated in a blue light- and phototropin-dependent manner. X-ray crystallography revealed that the CHUP1 C-terminal domain shares structural homology with the formin homology 2 (FH2) domain, despite lacking sequence similarity. Furthermore, the CHUP1 C-terminal domain promoted actin polymerization in the presence of profilin in vitro. Taken together, our findings indicate that CHUP1 is a plant-specific actin polymerization factor that has convergently evolved to assemble cp-actin filaments and enables chloroplast photorelocation movement.

-	~~Authors~~:	+	CHLOROPLAST UNUSUAL POSITIONING 1 is a plant-specific actin polymerization factor regulating chloroplast movement.,Kong SG, Yamazaki Y, Shimada A, T Kijima S, Hirose K, Katoh K, Ahn J, Song HG, Han JW, Higa T, Takano A, Nakamura Y, Suetsugu N, Kohda D, Uyeda TQP, Wada M Plant Cell. 2023 Dec 22:koad320. doi: 10.1093/plcell/koad320. PMID:38134410<ref>PMID:38134410</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8waf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
		+	[[Category: Large Structures]]
		+	[[Category: Kohda D]]
		+	[[Category: Nakamura Y]]
		+	[[Category: Shimada A]]
		+	[[Category: Takano A]]

OCA at 12:37, 18 October 2023

OCA — Wed, 18 Oct 2023 12:37:06 GMT

←Older revision		Revision as of 12:37, 18 October 2023
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8waf is ON HOLD	+	The entry 8waf is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8waf" [edit=sysop:move=sysop]

OCA — Wed, 20 Sep 2023 12:59:33 GMT

Protected "8waf" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:59, 20 September 2023

OCA: New page: '''Unreleased structure''' The entry 8waf is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Sep 2023 12:59:32 GMT

New page: '''Unreleased structure''' The entry 8waf is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8waf is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]