OCA at 08:07, 22 May 2024

OCA — Wed, 22 May 2024 08:07:45 GMT

←Older revision		Revision as of 08:07, 22 May 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8xf0 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the dissociated C-phycocyanin alpha-chain from Thermoleptolyngbya sp. O-77==
		+	<StructureSection load='8xf0' size='340' side='right'caption='[[8xf0]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8xf0]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptolyngbya_sp._O-77 Leptolyngbya sp. O-77]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XF0 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xf0 OCA], [https://pdbe.org/8xf0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xf0 RCSB], [https://www.ebi.ac.uk/pdbsum/8xf0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xf0 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	C-phycocyanin (CPC), which contains open-chain tetrapyrroles, is a major light-harvesting red-fluorescent protein with an important role in aquatic photosynthesis. Recently, we reported a non-conventional CPC from Thermoleptolyngbya sp. O-77 (CPC(O77)) that contains two different structures, i.e., a hexameric structure and a non-conventional octameric structure. However, the assembly and disassembly mechanisms of the non-conventional octameric form of CPC remain unclear. To understand this assembly mechanism, we performed an in vitro experiment to study the disassembly and reassembly behaviors of CPC using isolated CPC subunits. The dissociation of the CPC(O77) subunit was performed using a Phenyl-Sepharose column in 20 mM potassium phosphate buffer (pH 6.0) containing 7.0 M urea. For the first time, crystals of isolated CPC subunits were obtained and analyzed after separation. After the removal of urea from the purified alpha and beta subunits, we performed an in vitro reassembly experiment for CPC and analyzed the reconstructed CPC using spectrophotometric and X-ray crystal structure analyses. The crystal structure of the reassembled CPC was nearly identical to that of the original CPC(O77). The findings of this study indicate that the octameric CPC(O77) is a naturally occurring form in the thermophilic cyanobacterium Thermoleptolyngbya sp. O-77.

-	~~Authors:~~ Nguyen, ~~H.K.~~, Teramoto, T., ~~Kakuta~~, Y., Yoon~~, K~~.S.	+	Disassembly and reassembly of the non-conventional thermophilic C-phycocyanin.,Nguyen HK, Minato T, Teramoto T, Ogo S, Kakuta Y, Yoon KS J Biosci Bioeng. 2024 Mar;137(3):179-186. doi: 10.1016/j.jbiosc.2023.12.015. Epub , 2024 Jan 18. PMID:38238241<ref>PMID:38238241</ref>

-	~~Description: Crystal structure~~ of the ~~dissociated C-phycocyanin alpha-chain from Thermoleptolyngbya sp~~. O-77	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Nguyen, H~~.K]]	+	<div class="pdbe-citations 8xf0" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Yoon, K.S~~]]	+	== References ==
-	[[Category: ~~Kakuta, Y~~]]	+	<references/>
-	[[Category: Teramoto, T]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Leptolyngbya sp. O-77]]
		+	[[Category: Kakuta Y]]
		+	[[Category: Nguyen HK]]
		+	[[Category: Teramoto T]]
		+	[[Category: Yoon KS]]

OCA at 10:28, 24 January 2024

OCA — Wed, 24 Jan 2024 10:28:32 GMT

←Older revision		Revision as of 10:28, 24 January 2024
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8xf0 is ON HOLD	+	The entry 8xf0 is ON HOLD until Paper Publication

	Authors: Nguyen, H.K., Teramoto, T., Kakuta, Y., Yoon, K.S.		Authors: Nguyen, H.K., Teramoto, T., Kakuta, Y., Yoon, K.S.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Nguyen, H.K]]		[[Category: Nguyen, H.K]]
		+	[[Category: Yoon, K.S]]
	[[Category: Kakuta, Y]]		[[Category: Kakuta, Y]]
	[[Category: Teramoto, T]]		[[Category: Teramoto, T]]
-	[[Category: Yoon, K.S]]

OCA: Protected "8xf0" [edit=sysop:move=sysop]

OCA — Wed, 20 Dec 2023 09:09:58 GMT

Protected "8xf0" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:09, 20 December 2023

OCA: New page: '''Unreleased structure''' The entry 8xf0 is ON HOLD Authors: Nguyen, H.K., Teramoto, T., Kakuta, Y., Yoon, K.S. Description: Crystal structure of the dissociated C-phycocyanin alpha-c...

OCA — Wed, 20 Dec 2023 09:09:57 GMT

New page: '''Unreleased structure''' The entry 8xf0 is ON HOLD Authors: Nguyen, H.K., Teramoto, T., Kakuta, Y., Yoon, K.S. Description: Crystal structure of the dissociated C-phycocyanin alpha-c...

New page

'''Unreleased structure'''

The entry 8xf0 is ON HOLD

Authors: Nguyen, H.K., Teramoto, T., Kakuta, Y., Yoon, K.S.

Description: Crystal structure of the dissociated C-phycocyanin alpha-chain from Thermoleptolyngbya sp. O-77
[[Category: Unreleased Structures]]
[[Category: Nguyen, H.K]]
[[Category: Kakuta, Y]]
[[Category: Teramoto, T]]
[[Category: Yoon, K.S]]

8xf0 - Revision history

OCA at 08:07, 22 May 2024

OCA at 10:28, 24 January 2024

OCA: Protected "8xf0" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8xf0 is ON HOLD Authors: Nguyen, H.K., Teramoto, T., Kakuta, Y., Yoon, K.S. Description: Crystal structure of the dissociated C-phycocyanin alpha-c...