OCA at 08:07, 22 May 2024

OCA — Wed, 22 May 2024 08:07:54 GMT

←Older revision		Revision as of 08:07, 22 May 2024
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-	'''Unreleased structure'''

-	~~The entry~~ 8xjg is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the YqeY protein from Vibrio parahaemolyticus==
		+	<StructureSection load='8xjg' size='340' side='right'caption='[[8xjg]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[8xjg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_parahaemolyticus Vibrio parahaemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XJG FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xjg OCA], [https://pdbe.org/8xjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xjg RCSB], [https://www.ebi.ac.uk/pdbsum/8xjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xjg ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	YqeY is a functionally and structurally uncharacterized protein that is ubiquitously expressed in bacteria. To gain structural insights into the function of YqeY, we determined the crystal structures of the Campylobacter jejuni and Vibrio parahaemolyticus YqeY proteins (cjYqeY and vpYqeY, respectively) and analyzed the structural and functional roles of conserved residues via a mutational study. Both cjYqeY and vpYqeY were found to adopt a two-domain structure consisting of an N-terminal four-alpha-helix domain and a C-terminal three-alpha-helix domain, with a relatively flexible interdomain orientation. The YqeY structure is unique in its linkage of the two alpha-helix domains although the C-terminal YqeY domain is structurally homologous to the terminal appendages of glutaminyl-tRNA synthetase and tRNA-dependent amidotransferase. We identified six conserved YqeY residues (Y67, R72, E82, Y89, P91, and G119) and evaluated their roles in protein stability via alanine mutation using a thermal shift assay. Residues Y67, R72, Y89, and P91 were shown to be required to maintain the structural integrity of YqeY. In contrast, residues E82 and G119 were not found to be essential for protein stability and are highly likely to contribute to the biological function of YqeY.

-	~~Authors~~:	+	Structural analysis of the YqeY proteins from Campylobacter jejuni and Vibrio parahaemolyticus.,Kim SY, Yoon SI Biochem Biophys Res Commun. 2024 Feb 5;695:149485. doi: , 10.1016/j.bbrc.2024.149485. Epub 2024 Jan 5. PMID:38211535<ref>PMID:38211535</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 8xjg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Vibrio parahaemolyticus]]
		+	[[Category: Kim SY]]
		+	[[Category: Yoon SI]]

OCA at 09:10, 17 January 2024

OCA — Wed, 17 Jan 2024 09:10:17 GMT

←Older revision		Revision as of 09:10, 17 January 2024
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 8xjg is ON HOLD	+	The entry 8xjg is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8xjg" [edit=sysop:move=sysop]

OCA — Wed, 27 Dec 2023 22:25:43 GMT

Protected "8xjg" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:25, 27 December 2023

OCA: New page: '''Unreleased structure''' The entry 8xjg is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Dec 2023 22:25:42 GMT

New page: '''Unreleased structure''' The entry 8xjg is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8xjg is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

8xjg - Revision history

OCA at 08:07, 22 May 2024

OCA at 09:10, 17 January 2024

OCA: Protected "8xjg" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8xjg is ON HOLD Authors: Description: Category: Unreleased Structures